前扣带皮层δ-阿片受体介导电针缓解痛情绪的行为—电生理学观察
发布时间：2018-09-05 10:41
【摘要】：目的:慢性疼痛不仅是临床症状,同时也是一种疾病。疼痛包括痛感觉和痛情绪两种成分,前者反映疼痛的性质、强度和位置等属性,后者则包括了因痛而生的焦虑、厌恶、恐惧等不良情绪。随着人们对疼痛认识的深入,逐步发现慢性疼痛时往往痛情绪带来比痛感受本身更为严重的伤害,因此对痛情绪的研究也日益受到关注。来自人类和动物的行为学、电生理学和脑成像等相关研究表明,大脑额叶内侧前扣带皮层(anterior cingulate cortex,ACC)作为情绪情感环路中的重要核团,其吻侧部(rostral ACC,r ACC)在痛情绪的发生过程中起了十分重要的作用。众所周知,电针可以激活阿片受体产生明显的镇痛效应,但是否同样可以缓解痛情绪呢?这类资料目前还很少。我们先前的研究和相关实验室的资料都证实了电针也可以缓解痛情绪,而且还可通过激活μ-阿片受体和抑制NMDA受体对CFA诱导的条件位置回避(conditioned place avoidance,CPA)产生明显的缓解作用。阿片受体有多种亚型,其中最受关注的有μ、δ和κ三种阿片受体亚型。如前所述,我们已经证实μ-阿片受体参与介导了电针缓解痛情绪的作用,但其它受体亚型是否有类似的作用还不清楚。因此本研究将以δ-阿片受体为研究对象,利用大鼠脚掌注射CFA诱导的CPA(C-CPA)反应作为痛情绪行为模型,采用行为检测与多通道电生理学记录技术相结合的手段探索激活r ACC脑区δ-阿片受体是否可以起到缓解痛情绪的作用?以及r ACC脑区δ-阿片受体是否介导电针缓解痛情绪的过程。方法:自制给药管和带给药管的多通道电极,通过手术将自制的给药管和带给药管的多通道电极埋置于大鼠r ACC脑区,待术后一周大鼠完全恢复之后,给大鼠左侧后脚掌注射完全弗氏佐剂(CFA),诱导产生炎性痛并与环境相匹配,建立条件性位置回避(conditioned place avoidance,CPA)反应模型,r ACC脑区通过使用微量进样泵、PE给药管注射药物,通过大鼠脚掌回缩潜伏期(PWL)观察痛感受行为变化,通过条件位置回避装置观察痛情绪行为变化,同时利用在体多通道技术记录、处理、分析r ACC脑区神经元的信号变化。使用双因素进行分组,具体模式如下:12组动物(n=6-8只/组)=2(CFA,生理盐水)×6(δ-激动剂:5个剂量,生理盐水);8组动物(n=6-8只/组)=2(CFA,生理盐水)×2(δ-拮抗剂,生理盐水)×2(EA,sham EA)。结果:(1)大鼠建立条件位置回避(CPA)模型1)模型大鼠行为学观察脚掌热缩足反应潜伏期比较,CFA+NS组“痛环境”匹配后明显缩短,有统计学差异(P0.05),NS+NS组“痛环境”匹配前后没有差异(P0.05),大鼠后脚掌左侧注射CFA,产生炎症性疼痛。NS+NS组“痛环境”匹配前后在“痛环境”停留时间比较没有差异(P0.05),CFA+NS组“痛环境”匹配前后在“痛环境”停留时间比较,匹配后明显缩短,有统计学差异(P0.05);CFA+NS组与NS+NS组回避分数比较,前者产生明显的回避反应,有统计学差异(P0.05)。“痛环境”匹配后在“痛环境”停留时间明显缩短,而在“非痛环境”停留时间明显延长,对“痛环境”产生明显的回避,CFA诱导建立条件位置回避模型(CPA)。2)模型大鼠r ACC脑区神经元动作电位特征分析NS+NS组“痛环境”和“非痛环境”神经元放电频率比较,没有差异(P0.05)。CFA+NS组“痛环境”和“非痛环境”神经元放电频率比较,“痛环境”明显增高,有统计学差异(P0.05)。CFA+NS组与NS+NS组“痛环境”匹配之后在“痛环境”神经元放电频率比较,前者明显增高,有统计学差异(P0.05)。动作电位结果与行为一致。3)模型大鼠r ACC脑区局部场电位功率谱密度分析NS+NS组“痛环境”匹配后“痛环境”和“非痛环境”δ、θ、α、β、λ五种波的频率谱密度值比较,均无差异(P0.05)。CFA+NS组“痛环境”匹配后“痛环境”和“非痛环境”δ、θ、α、β、λ五种波的频率谱密度值比较,“痛环境”均明显增高有统计学差异(P0.05)。CFA+NS组与NS+NS组“痛环境”匹配后在“痛环境”δ、θ、α、β、λ五种频率波对应的频率谱密度值比较,前者均明显增高,有统计学差异(P0.05)。(2)激活大鼠r ACC脑区δ-阿片受体缓解大鼠痛情绪的行为-电生理学得观察1)大鼠r ACC脑区微量注射δ-阿片受体激动剂行为学观察CFA+DADLE(0.625/1.25/2.5/5/10μg/ul)组和CFA+NS组“痛环境”匹配前后在“痛环境”停留时间比较,CFA+DADLE(1.25/2.5/5/10μg/ul)组匹配前后均无差异(P0.05);CFA+DADLE(0.625μg/ul)组和CFA+NS组匹配前后,“痛环境”停留时间均明显缩短,有统计学差异(P0.05)。CFA+DADLE(0.625/1.25/2.5/5/10μg/ul)组分别与CFA+NS组回避分数比较,CFA+DADLE(0.625μg/ul)组和CFA+NS组比较没有差异(P0.05),CFA+DADLE(1.25/2.5/5/10μg/ul)组均没有产生明显的回避,CFA+DADLE(0.625μg/ul)组和CFA+NS组产生明显回避,有统计学差异(P0.05)。大鼠r ACC脑区注射不同浓度δ-阿片受体激动剂DADLE对CFA诱导产生的条件位置回避(CPA)产生不同影响,太低浓度DADLE对CPA没有明显作用,随着DADLE浓度的增加,对CPA有抑制作用,但抑制作用没有随DADLE浓度的增加而增强;低剂量的δ-阿片受体激动剂自身不会引起条件性奖赏。2)大鼠r ACC脑区微量注射δ-阿片受体激动剂神经元spike放电特征分析CFA+DADLE组“痛环境”和“非痛环境”神经元放电频率比较,没有差异(P0.05)。CFA+DADLE组与CFA+NS组“痛环境”匹配之后在“痛环境”神经元放电频率比较,CFA+NS组放电明显增多,有统计差异(P0.05)。NS+DADLE组“痛环境”和“非痛环境”神经元放电频率比较,没有差异(P0.05)。NS+DADLE组与NS+NS组“痛环境”匹配之后在“痛环境”神经元放电频率比较,没有差异(P0.05)。3)大鼠r ACC脑区微量注射δ-阿片受体激动剂神经元局部场电位变化及功率谱密度分析CFA+DADLE组和NS+DADLE组“痛环境”匹配后“痛环境”和“非痛环境”δ、θ、α、β、λ五种波的频率谱密度值比较,均无差异(P0.05)。CFA+DADLE组与CFA+NS组“痛环境”匹配后在“痛环境”δ、θ、α、β、λ五种频率波对应的频率谱密度值比较,CFA+NS组均明显增强,有统计学差异(P0.05)。(3)大鼠r ACC脑区δ-阿片受体介导电针缓解痛情绪作用的电生理学研究1)大鼠r ACC脑区给予不同浓度拮抗剂后,电针刺激大鼠环跳穴,对CFA诱导产生的条件位置回避反应(CPA)无明显作用。我们实验室已经完成,由其他同学详细描述。2)大鼠r ACC脑区微量注射δ-阿片受体拮抗剂后电针刺激环跳穴神经元spike放电特征分析NS+NS+sham EA组、CFA+NS+EA组、NS+Naltrindole+sham EA组“痛环境”和“非痛环境”神经元放电频率比较,没有差异(P0.05)。CFA+NS+sham EA组、CFA+Naltrindole+EA组“痛环境”和“非痛环境”神经元放电频率比较,“痛环境”神经元放电明显增强,有统计学差异(P0.05)。CFA+NS+EA组与CFA+NS+sham EA组“痛环境”匹配之后在“痛环境”神经元放电频率比较,CFA+NS+sham EA组明显增强,有统计学差异(P0.05)。CFA+Naltrindole+EA组与CFA+NS+EA组“痛环境”匹配之后在“痛环境”神经元放电频率比较,CFA+Naltrindole+EA组明显增强,有统计学差异(P0.05)。NS+Naltrindole+sham EA组与NS+NS+sham EA组“痛环境”匹配之后在“痛环境”神经元放电频率的比较,没有差异(P0.05)。3)大鼠r ACC脑区微量注射δ-阿片受体拮抗剂后电针刺激环跳穴神经元局部场电位变化及功率谱密度分析NS+NS+sham EA组、CFA+NS+EA组、NS+Naltrindole+sham EA组“痛环境”匹配后“痛环境”和“非痛环境”δ、θ、α、β、λ五种波的频率谱密度值比较,均无差异(P0.05)。CFA+NS+sham EA组、CFA+Naltrindole+EA组“痛环境”匹配后“痛环境”和“非痛环境”δ、θ、α、β、λ五种波的频率谱密度值比较,“痛环境”均明显增强,有统计学差异(P0.05)。CFA+NS+sham EA组与CFA+NS+EA组“痛环境”匹配后在“痛环境”δ、θ、α、β、λ五种频率波对应的频率谱密度值分别比较,CFA+NS+sham EA组均明显增强,有统计学差异(P0.05)。CFA+NS+EA组与CFA+Naltrindole+EA组“痛环境”匹配后在“痛环境”δ、θ、α、β、λ五种频率波对应的频率谱密度值比较,CFA+Naltrindole+EA组均明显增强,有统计学差异(P0.05)。NS+NS+sham EA组与NS+Naltrindole+sham EA组“痛环境”匹配后在“痛环境”δ、θ、α、β、λ五种频率波对应的频率谱密度值比较,均无差异(P0.05)。结论:通过以上结果可知:1)大鼠r ACC脑区注射δ-阿片受体激动剂DADLE,激活δ-阿片受体,减弱大鼠脚掌注射CFA产生的CPA反应,抑制CFA诱导的电活动;2)大鼠r ACC脑区注射δ-阿片受体拮抗剂,电针对CFA诱导的电活动的抑制作用被阻断;3)电针可激活大鼠r ACC脑区的δ-阿片受体,减弱CFA诱导产生的条件位置回避(CPA)反应,反转CFA诱导的电活动。
[Abstract]:Objective: Chronic pain is not only a clinical symptom, but also a disease. Pain includes pain sensation and pain emotion, the former reflects the nature, intensity and location of pain, the latter includes anxiety, aversion, fear and other adverse emotions caused by pain. Pain emotions are often more harmful than pain perception itself, so more and more attention has been paid to the study of pain emotions. Rostral ACC (r ACC) plays an important role in the development of pain. It is well known that electroacupuncture can activate opioid receptors to produce significant analgesic effects, but can it also alleviate pain? This kind of data is still very few. Our previous studies and related laboratory data have confirmed that electroacupuncture is also effective. Opioid receptors have a variety of subtypes, of which three opioid receptor subtypes, mu, Delta and kappa, are the most concerned. As mentioned above, we have confirmed mu-a. Tablet receptors mediate the analgesic effect of Electroacupuncture on pain, but it is not clear whether other receptor subtypes have similar effects. Therefore, this study will take the delta-opioid receptor as the research object, using the CPA (C-CPA) response induced by CFA injection into the paw of rats as a pain behavior model, using behavior detection and multi-channel electrophysiological recording techniques. To explore whether activating the delta-opioid receptor in the brain region of R ACC can alleviate pain and whether the delta-opioid receptor in the brain region of R ACC can mediate the process of alleviating pain by conducting needles. After a week of complete recovery, the rats were injected with CFA into the left hind paw of the rats to induce inflammatory pain and to match the environment. The conditioned place avoidance (CPA) reaction model was established. The changes of pain perception behavior were observed by the paw retraction latency (PWL) of rats, and the changes of pain emotion behavior were observed by the conditioned position avoidance device. At the same time, the signals of neurons in the R ACC brain region were recorded and processed by the in vivo multi-channel technique. The specific patterns were grouped by two factors as follows: 12 groups of animals (n=6-8 rats/group) = 2 (CFA, physiology). Results: (1) Comparing the latency of foot contraction in the CFA + NS group, CFA + NS group significantly contracted after matching the pain environment. There was no significant difference between the NS + NS group and the control group (P Comparing with NS + NS group, CFA + NS group had significant avoidance reaction, and there was significant difference (P 0.05). After matching, the residence time in "pain environment" was significantly shortened, while the residence time in "non-pain environment" was significantly prolonged, and the "pain environment" was significantly produced. To avoid, CFA induced conditioned place avoidance model (CPA). 2) Characteristic analysis of action potential of neurons in the R ACC area of rats in the CFA-induced conditioned place avoidance model (CPA). There was no significant difference in discharge frequencies between "pain environment" and "non-pain environment" neurons in NS+NS group (P 0.05). The discharge frequencies of "pain environment" and "non-pain environment" neurons in CFA+NS group were significantly different. Compared with NS + NS group, the firing frequency of neurons in "pain environment" was significantly higher in CFA + NS group after matching with NS + NS group (P 0.05). Action potential results were consistent with behavior. 3) Power spectral density analysis of R ACC brain area of model rats after matching with NS + NS group "pain environment" There was no significant difference in the frequency spectral densities between "pain environment" and "non-pain environment" (P 0.05). The frequency spectral densities of "pain environment" and "non-pain environment" (P 0.05) were significantly higher in CFA+NS group than in NS+N group (P 0.05). After matching the "pain environment" in group S, the corresponding spectral densities of delta, theta, alpha, beta, and lambda in the "pain environment" were significantly higher than those in the "pain environment" (P The retention time of CFA+DADLE(0.625/1.25/2.5/5/10 ug/ul) group and CFA+NS group before and after the matching of "pain environment" had no significant difference between CFA+DADLE(1.25/2.5/5/10 ug/ul) group and CFA+NS group before and after the matching of "pain environment"(P 0.05); There was no significant difference between CFA+DADLE (0.625/1.25/2.5/5/10 ug/ul) group and CFA+NS group (0.625 ug/ul) in avoidance score, CFA+DADLE (0.625 ug/ul) group and CFA+NS group (P 0.05), CFA+DADLE (1.25/2.5/5/10 ug/ul) group had no significant avoidance, CFA+DADLE (0.625 ug/ul) group and CFA+NS group had significant avoidance score, there was statistical difference. Different concentrations of delta-opioid receptor agonist DADLE had different effects on conditioned position avoidance (CPA) induced by CFA. Too low concentration of DADLE had no obvious effect on CPA. With the increase of DADLE concentration, it had inhibitory effect on CPA, but the inhibitory effect did not increase with the increase of DADLE concentration. The spike discharges of delta-opioid receptor agonist neurons injected into the R ACC area of rats showed no significant difference between the "pain environment" and "non-pain environment" neurons in the CFA+DADLE group (P 0.05). There was no significant difference between the "pain environment" and the "pain environment" in the CFA+DADLE group after matching the "pain environment" in the CFA+DADLE group with the "pain environment" in the CFA+NS group. There was no significant difference in the discharge frequencies of neurons in the "pain environment" and "non-pain environment" between the NS + DADLE group and the NS + NS group (P 0.05). There was no difference in the discharge frequencies of neurons in the "pain environment" between the NS + DADLE group and the NS + NS group (P 0.05). Changes of local field potentials and power spectral density of delta-opioid receptor agonist neurons after microinjection into the R ACC brain area of rats The frequency spectral densities of delta,theta,alpha,beta and lambda in the "pain environment" and "non-pain environment" after matching the "pain environment" of CFA+DADLE group and NS+DADLE group showed no difference (P 0.05). Comparing the spectral densities of delta, theta, alpha, beta and lambda frequencies in the "pain environment" matched with the "environment", the CFA + NS group showed a significant increase (P 0.05). (3) Electrophysiological study on the effect of delta-opioid receptor-mediated conductive acupuncture on pain relief in the R ACC brain region of rats (1) Electroacupuncture stimulation after different concentrations of antagonists were given to the R ACC brain region of rats. Our laboratory has been completed and described in detail by other students. 2) spike discharge characteristics of neurons stimulated by Electroacupuncture at acupoint Huantiao after microinjection of delta-opioid receptor antagonist in the R ACC area of rats. NS+NS+sham EA group, CFA+NS+EA group, NS+Naltrindole+sham EA group, NS+Naltrindole+sham EA group There was no significant difference in discharge frequencies between "pain environment" and "non-pain environment" neurons in EA group (P 0.05). The discharge frequencies of "pain environment" and "pain environment" neurons in CFA+NS+sham EA group, CFA+Naltrindole+EA group and "pain environment" neurons in "pain environment" were significantly higher than those in "pain environment" neurons in "pain environment" and "pain environment" neurons in "pain environment" group (P 0.05). Compared with the control group, the firing frequency of CFA + NS + sham EA group was significantly higher than that of CFA + NS + sham EA group (P 0.05). The firing frequency of CFA + Naltrindole + EA group was significantly higher than that of CFA + NS + EA group (P 0.05). There was no difference in the firing frequency of neurons in the "pain environment" between the rindole + sham EA group and NS + NS + sham EA group (P 0.05). There was no significant difference in the frequency spectral density between the "pain environment" and the "non-pain environment" after matching the "pain environment" in Naltrindole+sham EA group (P 0.05). The frequency spectral density ratio of the "pain environment" and the "non-pain environment" after matching the "pain environment" in CFA+NS+sham EA group and the "pain environment" in CFA+Naltrindole+EA group was higher than that in the "non-pain environment". Comparing with the pain environment of CFA+NS+sham EA group and CFA+NS+EA group, the frequency spectral density values of the five frequencies of delta, theta, alpha, beta and lambda in the pain environment of CFA+NS+sham EA group and CFA+NS+Naltrindole+EA group were significantly increased (P 0.05). Comparing the spectral densities of the five frequencies of the "pain environment" after matching, the CFA + Naltrindole + EA group was significantly higher than that of the NS + Naltrindole + Sham EA group (P 0.05). The frequencies of the five frequencies of the "pain environment" after matching were delta, theta, alpha, beta, and lambda in the "pain environment" of the NS + NS + sham EA group and the NS + Naltrindole + sham EA group. CONCLUSIONS: 1) Injecting DADLE into the R ACC brain area of rats, activating delta-opioid receptor, attenuating the CPA response and inhibiting the electrical activity induced by CFA, and 2) injecting delta-opioid receptor antagonist into the R ACC brain area of rats, electroacupuncture on the electrical activity induced by CFA. 3) EA could activate the delta-opioid receptor in the R ACC region of rats, weaken the conditioned position avoidance (CPA) response induced by CFA, and reverse the electrical activity induced by CFA.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R402
本文编号：2224026
[Abstract]:Objective: Chronic pain is not only a clinical symptom, but also a disease. Pain includes pain sensation and pain emotion, the former reflects the nature, intensity and location of pain, the latter includes anxiety, aversion, fear and other adverse emotions caused by pain. Pain emotions are often more harmful than pain perception itself, so more and more attention has been paid to the study of pain emotions. Rostral ACC (r ACC) plays an important role in the development of pain. It is well known that electroacupuncture can activate opioid receptors to produce significant analgesic effects, but can it also alleviate pain? This kind of data is still very few. Our previous studies and related laboratory data have confirmed that electroacupuncture is also effective. Opioid receptors have a variety of subtypes, of which three opioid receptor subtypes, mu, Delta and kappa, are the most concerned. As mentioned above, we have confirmed mu-a. Tablet receptors mediate the analgesic effect of Electroacupuncture on pain, but it is not clear whether other receptor subtypes have similar effects. Therefore, this study will take the delta-opioid receptor as the research object, using the CPA (C-CPA) response induced by CFA injection into the paw of rats as a pain behavior model, using behavior detection and multi-channel electrophysiological recording techniques. To explore whether activating the delta-opioid receptor in the brain region of R ACC can alleviate pain and whether the delta-opioid receptor in the brain region of R ACC can mediate the process of alleviating pain by conducting needles. After a week of complete recovery, the rats were injected with CFA into the left hind paw of the rats to induce inflammatory pain and to match the environment. The conditioned place avoidance (CPA) reaction model was established. The changes of pain perception behavior were observed by the paw retraction latency (PWL) of rats, and the changes of pain emotion behavior were observed by the conditioned position avoidance device. At the same time, the signals of neurons in the R ACC brain region were recorded and processed by the in vivo multi-channel technique. The specific patterns were grouped by two factors as follows: 12 groups of animals (n=6-8 rats/group) = 2 (CFA, physiology). Results: (1) Comparing the latency of foot contraction in the CFA + NS group, CFA + NS group significantly contracted after matching the pain environment. There was no significant difference between the NS + NS group and the control group (P Comparing with NS + NS group, CFA + NS group had significant avoidance reaction, and there was significant difference (P 0.05). After matching, the residence time in "pain environment" was significantly shortened, while the residence time in "non-pain environment" was significantly prolonged, and the "pain environment" was significantly produced. To avoid, CFA induced conditioned place avoidance model (CPA). 2) Characteristic analysis of action potential of neurons in the R ACC area of rats in the CFA-induced conditioned place avoidance model (CPA). There was no significant difference in discharge frequencies between "pain environment" and "non-pain environment" neurons in NS+NS group (P 0.05). The discharge frequencies of "pain environment" and "non-pain environment" neurons in CFA+NS group were significantly different. Compared with NS + NS group, the firing frequency of neurons in "pain environment" was significantly higher in CFA + NS group after matching with NS + NS group (P 0.05). Action potential results were consistent with behavior. 3) Power spectral density analysis of R ACC brain area of model rats after matching with NS + NS group "pain environment" There was no significant difference in the frequency spectral densities between "pain environment" and "non-pain environment" (P 0.05). The frequency spectral densities of "pain environment" and "non-pain environment" (P 0.05) were significantly higher in CFA+NS group than in NS+N group (P 0.05). After matching the "pain environment" in group S, the corresponding spectral densities of delta, theta, alpha, beta, and lambda in the "pain environment" were significantly higher than those in the "pain environment" (P The retention time of CFA+DADLE(0.625/1.25/2.5/5/10 ug/ul) group and CFA+NS group before and after the matching of "pain environment" had no significant difference between CFA+DADLE(1.25/2.5/5/10 ug/ul) group and CFA+NS group before and after the matching of "pain environment"(P 0.05); There was no significant difference between CFA+DADLE (0.625/1.25/2.5/5/10 ug/ul) group and CFA+NS group (0.625 ug/ul) in avoidance score, CFA+DADLE (0.625 ug/ul) group and CFA+NS group (P 0.05), CFA+DADLE (1.25/2.5/5/10 ug/ul) group had no significant avoidance, CFA+DADLE (0.625 ug/ul) group and CFA+NS group had significant avoidance score, there was statistical difference. Different concentrations of delta-opioid receptor agonist DADLE had different effects on conditioned position avoidance (CPA) induced by CFA. Too low concentration of DADLE had no obvious effect on CPA. With the increase of DADLE concentration, it had inhibitory effect on CPA, but the inhibitory effect did not increase with the increase of DADLE concentration. The spike discharges of delta-opioid receptor agonist neurons injected into the R ACC area of rats showed no significant difference between the "pain environment" and "non-pain environment" neurons in the CFA+DADLE group (P 0.05). There was no significant difference between the "pain environment" and the "pain environment" in the CFA+DADLE group after matching the "pain environment" in the CFA+DADLE group with the "pain environment" in the CFA+NS group. There was no significant difference in the discharge frequencies of neurons in the "pain environment" and "non-pain environment" between the NS + DADLE group and the NS + NS group (P 0.05). There was no difference in the discharge frequencies of neurons in the "pain environment" between the NS + DADLE group and the NS + NS group (P 0.05). Changes of local field potentials and power spectral density of delta-opioid receptor agonist neurons after microinjection into the R ACC brain area of rats The frequency spectral densities of delta,theta,alpha,beta and lambda in the "pain environment" and "non-pain environment" after matching the "pain environment" of CFA+DADLE group and NS+DADLE group showed no difference (P 0.05). Comparing the spectral densities of delta, theta, alpha, beta and lambda frequencies in the "pain environment" matched with the "environment", the CFA + NS group showed a significant increase (P 0.05). (3) Electrophysiological study on the effect of delta-opioid receptor-mediated conductive acupuncture on pain relief in the R ACC brain region of rats (1) Electroacupuncture stimulation after different concentrations of antagonists were given to the R ACC brain region of rats. Our laboratory has been completed and described in detail by other students. 2) spike discharge characteristics of neurons stimulated by Electroacupuncture at acupoint Huantiao after microinjection of delta-opioid receptor antagonist in the R ACC area of rats. NS+NS+sham EA group, CFA+NS+EA group, NS+Naltrindole+sham EA group, NS+Naltrindole+sham EA group There was no significant difference in discharge frequencies between "pain environment" and "non-pain environment" neurons in EA group (P 0.05). The discharge frequencies of "pain environment" and "pain environment" neurons in CFA+NS+sham EA group, CFA+Naltrindole+EA group and "pain environment" neurons in "pain environment" were significantly higher than those in "pain environment" neurons in "pain environment" and "pain environment" neurons in "pain environment" group (P 0.05). Compared with the control group, the firing frequency of CFA + NS + sham EA group was significantly higher than that of CFA + NS + sham EA group (P 0.05). The firing frequency of CFA + Naltrindole + EA group was significantly higher than that of CFA + NS + EA group (P 0.05). There was no difference in the firing frequency of neurons in the "pain environment" between the rindole + sham EA group and NS + NS + sham EA group (P 0.05). There was no significant difference in the frequency spectral density between the "pain environment" and the "non-pain environment" after matching the "pain environment" in Naltrindole+sham EA group (P 0.05). The frequency spectral density ratio of the "pain environment" and the "non-pain environment" after matching the "pain environment" in CFA+NS+sham EA group and the "pain environment" in CFA+Naltrindole+EA group was higher than that in the "non-pain environment". Comparing with the pain environment of CFA+NS+sham EA group and CFA+NS+EA group, the frequency spectral density values of the five frequencies of delta, theta, alpha, beta and lambda in the pain environment of CFA+NS+sham EA group and CFA+NS+Naltrindole+EA group were significantly increased (P 0.05). Comparing the spectral densities of the five frequencies of the "pain environment" after matching, the CFA + Naltrindole + EA group was significantly higher than that of the NS + Naltrindole + Sham EA group (P 0.05). The frequencies of the five frequencies of the "pain environment" after matching were delta, theta, alpha, beta, and lambda in the "pain environment" of the NS + NS + sham EA group and the NS + Naltrindole + sham EA group. CONCLUSIONS: 1) Injecting DADLE into the R ACC brain area of rats, activating delta-opioid receptor, attenuating the CPA response and inhibiting the electrical activity induced by CFA, and 2) injecting delta-opioid receptor antagonist into the R ACC brain area of rats, electroacupuncture on the electrical activity induced by CFA. 3) EA could activate the delta-opioid receptor in the R ACC region of rats, weaken the conditioned position avoidance (CPA) response induced by CFA, and reverse the electrical activity induced by CFA.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R402
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