1000～2500 nm宽波段的葡萄糖吸收光谱的测量波长优选

发布时间：2018-09-10 06:01

【摘要】：采用近红外光谱法无创测量人体血糖时,葡萄糖浓度变化引起的光强信号微弱,为了实现目标测量精度,需要选择适宜的测量波长使葡萄糖引起的吸收信号水平强于噪声信号水平.本文提出了基于最佳光程长的测量波长选择原则,可在测量灵敏度最大的条件下,评价葡萄糖吸收光谱的测量是否可行.以葡萄糖在水溶液中的测量为例,基于最佳光程长理论,对1 000~2 500 nm的每个波长均进行光程优化,以1 mmol/L的检测浓度为目标,筛选了可行的测量波段.结果表明,即使在最佳光程长下检测,该检测目标对测量光谱的重复性信噪比要求也非常高,当SNR达到10 000∶1时,仅有4个波段满足要求,分别是:1 000~1 160 nm,1 375~1 420 nm,1 590~1 640 nm和1 870~1 910 nm.其中短波长波段1 000~1 160 nm对光谱信噪比的要求最低,且最佳光程长最长,皮肤穿透深度最大,显示了较好的应用前景.这一结果对皮肤中的葡萄糖测量的波长选择有较好的参考价值.此外,本文所提出的波长选择方法也同样适用于采用吸收光谱法对其他物质成分的测量.
[Abstract]:In order to achieve the accuracy of target measurement, the signal of light intensity caused by the change of glucose concentration is weak when using near infrared spectroscopy (NIR) to measure human blood glucose noninvasively. It is necessary to choose the appropriate measurement wavelength to make the level of absorption signal caused by glucose stronger than that of noise signal. In this paper, the principle of wavelength selection based on optimal optical path length is proposed, which can be used to evaluate the feasibility of glucose absorption spectrum measurement under the condition of maximum measurement sensitivity. Taking the measurement of glucose in aqueous solution as an example, based on the theory of optimal optical path length, the optical path of each wavelength of 1 000 ~ (2 500) nm was optimized. The feasible wave band was screened with the detection concentration of 1 mmol/L as the target. The results show that even under the optimal optical path length detection, the target has a very high signal to noise ratio (SNR) requirement for the measurement spectrum. When the SNR reaches 10 000: 1, only 4 bands meet the requirements. They are: 1 000 1 160 nm,1 375 1 420 nm,1 590 1 640 nm and 1 870 1 910 nm. The spectral signal-to-noise ratio (SNR) is the lowest in the short wavelength band of 1 000 ~ 1 160 nm, and the best optical path is the longest and the skin penetration depth is the largest, which shows a good prospect of application. This result has good reference value for the wavelength selection of glucose measurement in skin. In addition, the wavelength selection method proposed in this paper is also suitable for the measurement of other substances by absorption spectrometry.
【作者单位】：精密测试技术及仪器国家重点实验室(天津大学);
【基金】：国家高技术研究发展计划(863计划)资助项目(2012AA022602,2012YQ090194) 国家自然科学基金资助项目(60938002,81471698)
【分类号】：O657.33;R446.1

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