Kim-1、PAI-1及Hsp72联合检测对糖尿病早期肾损害的诊断价值
发布时间：2019-04-22 21:53
【摘要】：目的探讨肾损伤分子(Kim-1)、人中性粒细胞明胶酶相关脂质运载蛋白(NGAL)、人纤溶酶原激活物抑制因子-1(PAI-1)及热休克蛋白72(Hsp72)联合检测对糖尿病早期肾损害的诊断价值。方法将180例2型糖尿病患者按尿蛋白水平分为尿蛋白阴性组(n=58)和尿蛋白阳性组(n=122),以同时期60例尿蛋白检查阴性的健康体检者为对照组。采用ELISA法检测各组尿Kim-1、NGAL、PAI-1及Hsp72水平,采用ROC曲线分析各标志物单一及不同组合对糖尿病早期肾损害的诊断价值。结果各组间Kim-1、NGAL、PAI-1及Hsp72水平存在显著差异(P0.01),以尿蛋白阳性组水平最高,尿蛋白阴性组次之,对照组最低。单一标志物检测时,Kim-1敏感度最强(77.2%),Hsp72特异度最强(90.0%),二者ROC曲线下面积(AUC)分别为0.879和0.877。两项联合检测中,Kim-1+Hsp72敏感度最强(90.6%),Kim-1+PAI-1特异度最强(96.7%);三项联合检测中,Kim-1+PAI-1+Hsp72的敏感度和特异度最高,分别为91.1%和97.0%。结论 Kim-1和Hsp72联合检测在糖尿病早期肾损害诊断中具有较高的敏感度和特异度,必要时可联合PAI-1进行检测,以提高诊断的准确性。
[Abstract]:Objective to investigate renal injury molecule (Kim-1), human neutrophil gelatinase-associated lipid delivery protein (NGAL),). The diagnostic value of combined detection of human plasminogen activator inhibitor-1 (PAI-1) and heat shock protein 72 (Hsp72) in early diabetic nephropathy. Methods 180 patients with type 2 diabetes mellitus were divided into two groups according to urinary protein level: urine protein negative group (n = 58) and urine protein positive group (n = 122). 60 healthy subjects with negative urine protein test were used as control group at the same time. The levels of urinary Kim-1,NGAL,PAI-1 and Hsp72 in each group were measured by ELISA method. The diagnostic value of single marker and different combination of each marker in early renal damage of diabetes mellitus was analyzed by ROC curve. Results there were significant differences in the levels of Kim-1,NGAL,PAI-1 and Hsp72 among the three groups (P0.01). The urinary protein positive group was the highest, the urine protein negative group was the second, and the control group was the lowest. The sensitivity of Kim-1 was the strongest (77.2%) and the specificity of Hsp72 was the strongest (90.0%). The area (AUC) under ROC curve was 0.879 and 0.877 respectively. The sensitivity of Kim-1 Hsp72 was the strongest (90.6%) and the specificity of Kim-1 PAI-1 was the strongest (96.7%). The sensitivity and specificity of Kim-1 PAI-1 Hsp72 were 91. 1% and 97. 0% respectively. Conclusion the combined detection of Kim-1 and Hsp72 has high sensitivity and specificity in the diagnosis of early diabetic renal damage. If necessary, it can be detected in combination with PAI-1 in order to improve the accuracy of diagnosis.
【作者单位】： 琼海市人民医院检验科;琼海市人民医院内分泌科;
【分类号】：R587.2;R692.9;R446.1
[Abstract]:Objective to investigate renal injury molecule (Kim-1), human neutrophil gelatinase-associated lipid delivery protein (NGAL),). The diagnostic value of combined detection of human plasminogen activator inhibitor-1 (PAI-1) and heat shock protein 72 (Hsp72) in early diabetic nephropathy. Methods 180 patients with type 2 diabetes mellitus were divided into two groups according to urinary protein level: urine protein negative group (n = 58) and urine protein positive group (n = 122). 60 healthy subjects with negative urine protein test were used as control group at the same time. The levels of urinary Kim-1,NGAL,PAI-1 and Hsp72 in each group were measured by ELISA method. The diagnostic value of single marker and different combination of each marker in early renal damage of diabetes mellitus was analyzed by ROC curve. Results there were significant differences in the levels of Kim-1,NGAL,PAI-1 and Hsp72 among the three groups (P0.01). The urinary protein positive group was the highest, the urine protein negative group was the second, and the control group was the lowest. The sensitivity of Kim-1 was the strongest (77.2%) and the specificity of Hsp72 was the strongest (90.0%). The area (AUC) under ROC curve was 0.879 and 0.877 respectively. The sensitivity of Kim-1 Hsp72 was the strongest (90.6%) and the specificity of Kim-1 PAI-1 was the strongest (96.7%). The sensitivity and specificity of Kim-1 PAI-1 Hsp72 were 91. 1% and 97. 0% respectively. Conclusion the combined detection of Kim-1 and Hsp72 has high sensitivity and specificity in the diagnosis of early diabetic renal damage. If necessary, it can be detected in combination with PAI-1 in order to improve the accuracy of diagnosis.
【作者单位】： 琼海市人民医院检验科;琼海市人民医院内分泌科;
【分类号】：R587.2;R692.9;R446.1
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