血液质量控制检测项目试剂盒的选择及室内质量控制方法的建立

发布时间：2019-04-26 03:09

【摘要】：目的评估血液制品中甘油、上清蛋白、血浆蛋白、游离Hb以及亚甲蓝检测试剂盒的可行性并建立室内质量控制。方法选择市售生化试剂盒,自制室内质控品并制定质量控制范围,分别用分光光度计和生化仪器同时对血液制品中的甘油、上清蛋白、血浆蛋白、游离Hb以及亚甲蓝检测进行检测,甘油检测采用GPO-PAP酶比色法,上清蛋白采用邻笨三酚红比色法,血浆蛋白采用双缩脲比色法,游离Hb采用Trinder比色法,亚甲蓝采用萃取比色法。结果选出5种试剂盒并建立了室内质控范围;甘油、上清蛋白、血浆蛋白、游离Hb以及亚甲蓝2种仪器比对检测结果均相近(P0.05);甘油、上清蛋白、血浆蛋白、游离Hb以及亚甲蓝用分光度计各检测48人份,其最高值分别为2.12 g/L、2.40 g/L、63.8 g/L、1.09 g/L、0.31μmol/L。结论血液质量控制项目可采用试剂盒可在分光光度计上进行检测,简单可行,建立室内质控,确保结果准确性。
[Abstract]:Objective to evaluate the feasibility of determination of glycerol, albumin, plasma protein, free Hb and methylene blue in blood products and establish indoor quality control. Methods the biochemical kit was selected and the quality control range was drawn up. The glycerol, supernatant protein and plasma protein in blood products were determined by spectrophotometer and biochemical instrument, respectively. Free Hb and methylene blue were detected, glycerol was detected by GPO-PAP enzyme colorimetry, supernatant protein by pyrogallol red colorimetry, plasma protein by biuret colorimetry, free Hb by Trinder colorimetry. Methylene blue was determined by extraction colorimetry. Results five kinds of kits were selected and the range of indoor quality control was established, and the results were similar to those of glycyrrhizin, supernatant protein, plasma protein, free Hb and methylene blue (P0.05). The maximum values of glycerol, albumin, plasma protein, free Hb and methylene blue were 2.12 g / L, 2.40 g / L, 63.8 g / L, 1.09 g / L, 0.31 渭 mol / L respectively, and the highest values were 2.12 g / L, 2.40 g / L, 63.8 g / L, 1.09 g / L, 0.31 渭 mol / L respectively. Conclusion Blood quality control items can be detected in spectrophotometer by reagent kit. It is simple and feasible to establish indoor quality control to ensure the accuracy of the results.
【作者单位】：河南省红十字血液中心;
【分类号】：R446.1

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