福建省begomoviruses的检测和分子生物学研究
发布时间：2020-12-08 04:09
双生病毒是一类重要的植物病毒,病毒粒子呈典型的孪生状,基因组由单链环状DNA构成。当前,双生病毒科被分为9个属,分别为Becurtovirus,Begomovirus,Capulavirus,Curtovirus,Eragrovirus,Grablovirus,Mastrevirus,Topocuvirus 和 Turncurtovirus。双生病毒,特别是begomovirus,在热带和亚热带地区严重危害。在一些欠发达国家和地区,它们甚至会造成饥荒。近年来,begomovirus的发生在中国南方越来越普遍。然而,这类病毒在福建省的发生情况还不甚清楚。在第一部分,本研究于2015-2017年间,从福建各地采集了疑似begomovirus侵染的朱槿、玫瑰茄、黄秋葵、黄麻、芒麻、番茄和胜红蓟等植物样本。利用简并引物PCR并结合必要的全基因组克隆,本研究从这些样本中鉴定到6种begomovirus,分别为木尔坦棉花曲叶病毒（CLCuMuV）、番茄黄化曲叶病毒（TYLCV）、越南黄麻黄脉病毒（COYVV）,苎麻花叶病毒（RamV）、中国胜红蓟黄脉病毒（AYVCnV）和野葛花叶病毒（KuMV）。...
【文章来源】：福建农林大学福建省
【文章页数】：115 页
【学位级别】：博士
【文章目录】：
List of Abbreviations
摘要
Abstract
Chapter 1 Introduction and Review of literature
1.1 Geminiviruses: General description
1.2 Geminiviruses:Txonmy
1.3 Geminiviruses: Genome organization
1.4 Geminiviruses: gene function and host-virus interaction
1.5 Geminiviruses: Ecology
1.6 Geminiviruses in China and Fujian Province
1.7 Objectives of Research
Chapter 2 Detection of Begomoviruses from different host plants in Fujian Province ofChina
2.1 Background
2.2 Materials and methods
2.2.1 Collection of samples
2.2.2 Genomic DNA extraction
2.2.3 PCR amplification, cloning, and sequencing
2.2.4 PCR/RCA amplification of DNA P
2.2.5 Phylogenetic analysis
2.3 Results and Discussion
2.3.1 Identification of Begomoviruses and Satellites in Fujian Province of China
2.3.1.1 Detection of Cotton leaf curl Multan virus and Betasatellite from Hibiscussabdariffa
2.3.1.2 Detection of Ramie mosaic virus （RamV） from Ramie （Boehmeria nivea）
2.3.1.3 Detection of Tomato yellow leaf curl virus （TYLCV） from Tomato plants（Lycopersicon esculentum L）
2.3.1.4 Detection of Corchorus yellow vein Vietnam virus （CoYVV） from Jute plants（Corchorus olitorius L.）
2.3.1.5 Detection of Ageratum yellow vein China virus （AYVCnV） infecting Ageratum（Ageratum conyzoides）
2.3.1.6 Detection of Kudzu mosaic virus virus （KuMV） infecting Kudzu （Ageratumconyzoides）
Chapter 3 Construction of Infectious clone and Molecular studies/characterization ofCLCuMuV, RamV and CoYVV
3.1 Background
3.2 Material and Methods
3.2.1 Collection of samples and DNA extraction
3.2.2 Construction of infectious clone and agrobacterium infilteration
3.2.3 Confirmation of infection by Southern Blot analysis
3.2.4 Cloning and sequence analysis:
3.3 Results and Discussion
Chapter 4 Molecular characterization and RSV Co-infect Nicotiana benthamiana withbegomoviruses
4.1 Background
4.2 Material and Methods
4.2.1 Virus Source and inoculation
4.2.2 Detection of TSSs by Southern Blot analysis
4.2.3 Primer Design
4.2.4 High Throughput sequencing of 5' ends of CLCuMuV, RamV and CoYVVNCP
4.3 Results and Discussion
4.3.1 Rice stripe virus Co-infect Nicotiana benthamina with Cotton leaf curl Multan virus（CLCuMuV）, Ramie mosaic virus （RamV）, Corchorus yellow vein Vietnam virus（CoYVV）
4.3.2 RSV snatches capped RNA leaders from CLCuMuV, RamV and CoYVVmRNAs
Chapter 5 General Discussion
Chapter 6 Conclusions and Future perspective of this study
6.1 Conclusion
6.2 Future Perspective
References
Published Papers and other Academic Achievements during the Study
Acknowledgement
Appendices
Appendix A
Appendix B
【参考文献】：
期刊论文
[1]传播木尔坦棉花曲叶病毒的烟粉虱隐种鉴定[J]. 陈婷,汤亚飞,赵蕊,何自福,吕利华. 植物保护学报. 2016(01)
[2]海南红麻曲叶病的病原检测与鉴定[J]. 汤亚飞,何自福,杜振国,佘小漫,蓝国兵. 植物病理学报. 2015(06)
[3]侵染垂花悬铃花的木尔坦棉花曲叶病毒分子特征研究[J]. 汤亚飞,何自福,杜振国,韩利芳,佘小漫,罗方芳. 植物病理学报. 2013(02)
[4]福州市发生由木尔坦棉花曲叶病毒引起的朱槿曲叶病[J]. 章松柏,夏宣喜,张洁,张友军,吴祖建. 植物保护. 2013(02)
[5]侵染广东黄秋葵的木尔坦棉花曲叶病毒及伴随卫星DNA的分子特征[J]. 董迪,朱艳华,何自福,柴兆祥,佘小漫,罗方芳. 华南农业大学学报. 2012(01)
[6]北京地区番茄黄化曲叶病毒病的鉴定及防治对策[J]. 周涛,师迎春,陈笑瑜,范在丰. 植物保护. 2010(02)
[7]江苏省番茄黄化曲叶病的病原分子诊断[J]. 季英华,熊如意,程兆榜,周彤,赵统敏,余文贵,范永坚,周益军. 园艺学报. 2008(12)
[8]侵染朱槿的木尔坦棉花曲叶病毒及其卫星DNA全基因组结构特征[J]. 毛明杰,何自福,虞皓,李华平. 病毒学报. 2008(01)
[9]鲤肠黄脉病样中烟粉虱传双生病毒的检测[J]. 何自福,虞皓,毛明杰,佘小漫. 西北农林科技大学学报(自然科学版). 2005(S1)
[10]广东番茄曲叶病毒G3分离物基因组DNA-A的分子特征[J]. 何自福,虞皓,罗方芳. 植物病理学报. 2005(03)
本文编号：2904428
【文章来源】：福建农林大学福建省
【文章页数】：115 页
【学位级别】：博士
【文章目录】：
List of Abbreviations
摘要
Abstract
Chapter 1 Introduction and Review of literature
1.1 Geminiviruses: General description
1.2 Geminiviruses:Txonmy
1.3 Geminiviruses: Genome organization
1.4 Geminiviruses: gene function and host-virus interaction
1.5 Geminiviruses: Ecology
1.6 Geminiviruses in China and Fujian Province
1.7 Objectives of Research
Chapter 2 Detection of Begomoviruses from different host plants in Fujian Province ofChina
2.1 Background
2.2 Materials and methods
2.2.1 Collection of samples
2.2.2 Genomic DNA extraction
2.2.3 PCR amplification, cloning, and sequencing
2.2.4 PCR/RCA amplification of DNA P
2.2.5 Phylogenetic analysis
2.3 Results and Discussion
2.3.1 Identification of Begomoviruses and Satellites in Fujian Province of China
2.3.1.1 Detection of Cotton leaf curl Multan virus and Betasatellite from Hibiscussabdariffa
2.3.1.2 Detection of Ramie mosaic virus （RamV） from Ramie （Boehmeria nivea）
2.3.1.3 Detection of Tomato yellow leaf curl virus （TYLCV） from Tomato plants（Lycopersicon esculentum L）
2.3.1.4 Detection of Corchorus yellow vein Vietnam virus （CoYVV） from Jute plants（Corchorus olitorius L.）
2.3.1.5 Detection of Ageratum yellow vein China virus （AYVCnV） infecting Ageratum（Ageratum conyzoides）
2.3.1.6 Detection of Kudzu mosaic virus virus （KuMV） infecting Kudzu （Ageratumconyzoides）
Chapter 3 Construction of Infectious clone and Molecular studies/characterization ofCLCuMuV, RamV and CoYVV
3.1 Background
3.2 Material and Methods
3.2.1 Collection of samples and DNA extraction
3.2.2 Construction of infectious clone and agrobacterium infilteration
3.2.3 Confirmation of infection by Southern Blot analysis
3.2.4 Cloning and sequence analysis:
3.3 Results and Discussion
Chapter 4 Molecular characterization and RSV Co-infect Nicotiana benthamiana withbegomoviruses
4.1 Background
4.2 Material and Methods
4.2.1 Virus Source and inoculation
4.2.2 Detection of TSSs by Southern Blot analysis
4.2.3 Primer Design
4.2.4 High Throughput sequencing of 5' ends of CLCuMuV, RamV and CoYVVNCP
4.3 Results and Discussion
4.3.1 Rice stripe virus Co-infect Nicotiana benthamina with Cotton leaf curl Multan virus（CLCuMuV）, Ramie mosaic virus （RamV）, Corchorus yellow vein Vietnam virus（CoYVV）
4.3.2 RSV snatches capped RNA leaders from CLCuMuV, RamV and CoYVVmRNAs
Chapter 5 General Discussion
Chapter 6 Conclusions and Future perspective of this study
6.1 Conclusion
6.2 Future Perspective
References
Published Papers and other Academic Achievements during the Study
Acknowledgement
Appendices
Appendix A
Appendix B
【参考文献】：
期刊论文
[1]传播木尔坦棉花曲叶病毒的烟粉虱隐种鉴定[J]. 陈婷,汤亚飞,赵蕊,何自福,吕利华. 植物保护学报. 2016(01)
[2]海南红麻曲叶病的病原检测与鉴定[J]. 汤亚飞,何自福,杜振国,佘小漫,蓝国兵. 植物病理学报. 2015(06)
[3]侵染垂花悬铃花的木尔坦棉花曲叶病毒分子特征研究[J]. 汤亚飞,何自福,杜振国,韩利芳,佘小漫,罗方芳. 植物病理学报. 2013(02)
[4]福州市发生由木尔坦棉花曲叶病毒引起的朱槿曲叶病[J]. 章松柏,夏宣喜,张洁,张友军,吴祖建. 植物保护. 2013(02)
[5]侵染广东黄秋葵的木尔坦棉花曲叶病毒及伴随卫星DNA的分子特征[J]. 董迪,朱艳华,何自福,柴兆祥,佘小漫,罗方芳. 华南农业大学学报. 2012(01)
[6]北京地区番茄黄化曲叶病毒病的鉴定及防治对策[J]. 周涛,师迎春,陈笑瑜,范在丰. 植物保护. 2010(02)
[7]江苏省番茄黄化曲叶病的病原分子诊断[J]. 季英华,熊如意,程兆榜,周彤,赵统敏,余文贵,范永坚,周益军. 园艺学报. 2008(12)
[8]侵染朱槿的木尔坦棉花曲叶病毒及其卫星DNA全基因组结构特征[J]. 毛明杰,何自福,虞皓,李华平. 病毒学报. 2008(01)
[9]鲤肠黄脉病样中烟粉虱传双生病毒的检测[J]. 何自福,虞皓,毛明杰,佘小漫. 西北农林科技大学学报(自然科学版). 2005(S1)
[10]广东番茄曲叶病毒G3分离物基因组DNA-A的分子特征[J]. 何自福,虞皓,罗方芳. 植物病理学报. 2005(03)
本文编号：2904428
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