水稻根系相关内生细菌的分离鉴定及生防潜能研究

发布时间：2021-06-26 03:13

　　水稻（Oryza sativa L.）是世界上最重要的粮食作物之一。水稻在生长的过程中受到各种病原菌的影响,其中植物病原真菌可使水稻产生多种重要的病害,这些水稻疾病的爆发导致严重的经济损失。目前防治这些水稻病害的主要途径是使用化学农药,然而化学农药长期、大量不合理的使用导致其产生的抗药性,抗药性菌株的出现已成为病害频繁爆发的最主要因素。随着经济社会的快速发展,人们对农产品质量安全和生态环境的要求越来越高,这迫使我国必须尽快研究和开发出替代化学农药的绿色安全高效生物防治制剂以有效地防治水稻病害。生防微生物及其代谢产物具有防效高、环境友好且对人畜无毒等优点,是一类理想的可替代化学农药的产品。根系内的内生细菌及其代谢产物具有抑制植物病原菌的活性,从而增强植物对病原菌的抵抗力。许多内生细菌由于对植物病原菌具有良好的抑制活性,已经被开发成生物防治剂在田间广泛使用。然而,水稻根系内生细菌对植物病原真菌的研究却鲜有报道。本研究选取水稻田间不同品种的健康植株,对水稻根系的内生细菌进行了分离鉴定,测定了内生细菌对几种重要水稻真菌病原物的抑制活性,并对内生细菌生防机制进行了初步探究,具体的研究结果如下:1... 

【文章来源】：中国农业科学院北京市

【文章页数】：131 页

【学位级别】：博士

【文章目录】：
摘要
ABSTRACT
LIST OF ABBREVIATIONS
CHAPTER 1 INTRODUCTION
    1.1 GENERAL INTRODUCTION
    1.2 REVIEW LITERATURE
        1.2.1 Introduction of bacterial endophytes
        1.2.2 Diversity of bacterial endophytes
        1.2.3 The Lifestyles of the bacterial endophytes
        1.2.4 Colonization behavior of bacterial endophytes
        1.2.5 Colonization mechanism of the bacterial endophytes in the host plant
        1.2.6 Function and role of plant bacterial endophytes
        1.2.7 Evolution of bacterial endophytes in plant by symbioses
        1.2.8 Biocontrol mechanism of endophytic bacterial
        1.2.9 Antagonistic mechanisms of bacterial endophytes by using biocontrol agents
        1.2.10 Secondary metabolite producing ability of bacterial endophytes
        1.2.11 Biotic and abiotic stresses tolerance abilities of bacterial endophytes
        1.2.12 Bacterial endophytes suppressing plant phytopathogens by anti-biotics and anti-biosis
        1.2.13 Induced systemic resistance(ISR)by bacterial endophytes against plant phytopathogens
CHAPTER 2 ISOLATION AND CHARACTERIZATION OF ROOT-ASSOCIATED BACTERIAL ENDOPHYTES AND THEIR BIOCONTROL POTENTIAL AGAINST MAJOR FUNGAL PHYTOPATHOGENS OF RICE(ORYZA SATIVA L.)
    2.1 INTRODUCTION
    2.2 MATERIAL AND METHODS
        2.2.1 Plant material and samples locations
        2.2.2 Surface sterilization of rice root tissues
        2.2.3 Isolation of root-associated bacterial endophytes
        2.2.4 Biochemical and physiological characterization of root-associated bacterial endophytes
        2.2.5 Genomic DNA extraction of root-associated bacterial endophytes
        2.2.6 PCR amplification and classification of root-associated bacterial endophytes
        2.2.7 Phylogenetic analysis of root-associated bacterial endophytes based on16s r RNA gene sequencings
        2.2.8 Rice Fungal Phytopathogens and Culture Condition
        2.2.9 Molecular detection of antibiotic related genes based on PCR amplification of root-associated bacterial endophytes
        2.2.10 Statistical Analysis
    2.3 RESULTS
        2.3.1 Isolation and identification of root-associated bacterial endophytes
        2.3.2 Molecular classification and phylogenetic association of root-associated bacterial endophytes
        2.3.3 Biochemical and physiological characterization of root-associated bacterial endophytes
        2.3.4 In-vitro antagonistic activities of root-associated bacterial endophytes
        2.3.5 Molecular detection of antibiotic related genes based on PCR amplification of root-associated bacterial endophytes
    2.4 DISCUSSION
    2.5 CONCLUSION
CHAPTER3 MAJOR FINDINGS AND FUTURE PROSPECTIVES
    3.1 MAJOR FINDINGS
    3.2 FUTURE PROSPECTIVES
REFERENCES
APPENDIX
LIST OF PUBLICATIONS
ACKNOWLEDGEMENTS
AUTHORS RESUME


【参考文献】：
期刊论文
[1]Gram-Positive Bacteria Associated with Rice in China and Their Antagonists Against the Pathogens of Sheath Blight and Bakanae Disease in Rice[J]. LUO Jin-yan1, XIE Guan-lin1, LI Bin1, LUO Yuan-chan1, ZHAO Li-han1, WANG Xiao 1, LIU Bo2, LI Wen1 (1 State Key Laboratory of Rice Biology, Zhejiang University,Hangzhou 310029, China; 2 Center of Biotechnology, Fujian Academy of Agricultural Science, Fuzhou 350003, China).  Rice Science. 2005(03)



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