蜜蜂咽下腺蛋白质组的深度分析

发布时间：2021-09-18 11:29

　　蜂王浆是工蜂咽下腺等腺体分泌的浆状物质,主要用以饲喂蜜蜂小幼虫和蜂王。为了提高蜂王浆产量,我国从意大利蜜蜂（意蜂）中成功培育出蜂王浆高产蜜蜂（浆蜂）,蜂王浆产量至少提高了10倍。基于蛋白质组学技术,咽下腺发育和功能的分子机制研究已广泛开展,为蜂王浆高产机制的解析提供了科学依据。但受过去蛋白质组学发展技术的制约,只有少部分蛋白质得以鉴定和分析,因此,随着以质谱技术为主蛋白组学技术的快速发展,需要借助新技术对蜜蜂蛋白质谱进行深入系统研究。本研究比较了浆蜂和意蜂的新出房蜂、哺育蜂和采集蜂的咽下腺蛋白质组,在去除咽下腺里435个分泌蛋白后,即蜂王浆蛋白,在浆蜂和意蜂的咽下腺中共鉴定了5,751个蛋白,约占蜜蜂总蛋白组覆盖率的38%,显著提高咽下腺蛋白质组覆盖深度。功能富集分析结果表明,不同日龄咽下腺鉴定的蛋白和上调表达蛋白在浆蜂和意蜂中所鉴定蛋白和差异表达主要参与蛋白质合成和糖类代谢支撑了腺体在不同阶段的基本生物学需求,如幼蜂时期的腺体发育,哺育和采集蜂时期的蜂王浆蛋白质和花蜜转化酶分泌等的需求。然而浆蜂和意蜂咽下腺在不同发育阶段都具有发育阶段特异的蛋白表达谱帮助腺体实现各个阶段的生物学功能。... 

【文章来源】：中国农业科学院北京市

【文章页数】：138 页

【学位级别】：博士

【文章目录】：
摘要
ABSTRACT
ABBREVIATIONS
CHAPTER1 INTRODUCTION
    1. The honey bees
        1.1 The beekeeping industry
        1.2 Chinese beekeeping industry
        1.3 Economic importance of the honeybee
            1.3.1 Honey
            1.3.2 Beeswax
            1.3.3 Royal Jelly
            1.3.4 Pollen
            1.3.5 Other products
            1.3.6 Pollination
        1.4 The honeybee colony and the cast dependent role of worker bees
            1.4.1 The honeybee queen
            1.4.2 The honeybee drones
            1.4.3 The honeybee workers
        1.5 Anatomy of the honeybee
            1.5.1 The Hypopharyngeal Glands (HGs)
CHAPTER2 QUALITATIVE AND QUANTITATIVE PROTEOME COMPARISON OF HYPOPHARYNGEALGLANDS (HGs) OF ITALIAN HONEYBEES (APIS MELLIFERA) AT DIFFERENT AGES
    2.1 SUMMARY
    2.2 INTRODUCTION
    2.3 OBJECTIVE
    2.4 MATERIALS AND METHOD
        2.4.1 Reagents
        2.4.2 Sampling hypopharyngeal glands of ITBs
        2.4.3 Protein extraction and digestion
        2.4.4 LC?MS/MS analysis
        2.4.5 Identification and abundance level quantitation of proteins
        2.4.6 Bioinformatics analysis
    2.5 RESULTS
        2.5.1 Qualitative proteome comparison of HG development in ITBs
        2.5.2 Quantitative proteome comparison of HG development in ITB
    2.6 DISCUSSION
        2.6.1 Proper development and basic functions of the HGs requires shared proteome
        2.6.2 Age-dependent development and functions of the HGs requires age-specific proteins
    2.7 CONCLUSION
CHAPTER3 QUALITATIVE AND QUANTITATIVE PROTEOME COMPARISON OF HYPOPHARYNGEAL GLANDS (HGs) OF HIGH ROYAL JELLY PRODUCING HONEYBEES (RJBs) (APIS MELLIFERALIGUSTICA) AT DIFFERENT AGES
    3.1 SUMMARY
    3.2 INTRODUCTION
    3.3 OBJECTIVE
    3.4 MATERIALS and METHODS
        3.4.1 Reagents
        3.4.2 Sampling hypopharyngeal glands of RJBs
    3.5 RESULTS
        3.5.1 Qualitative proteome comparison of HG development in RJBs
        3.5.2 Quantitative proteome comparison of HG development in RJBs
    3.6 Discussion
        3.6.1 Proper development and basic functions of the HGs requires shared proteome
        3.6.2 Age-dependent development and functions of the HGs requires age-specific Proteins
    3.7 CONCLUSION
CHAPTER4 PROTEOME COMPARISON OF HYPOPHARYNGEAL GLANDS (HGS) BETWEEN TWO STOCKS OF HONEYBEES REVEALS DEEP INSIGHT INTO MOLECULAR UNDERLINGS OF HGDEVELOPMENT, FUNCTIONALITY AND DIFFERENCE
    4.1 SUMMARY
    4.2 INTRODUCTION
    4.3 OBJECTIVE
    4.4 MATERIALS AND METHOD
        4.4.1 Reagents
        4.4.2 Sampling hypopharyngeal lands from ITBs and RJBs
    4.5 RESULT
        4.5.1 Proteome comparison of HG between NEBs from ITBs and RJBs
        4.5.2 Proteome comparison of HG between NBs from ITBs and RJBs
        4.5.3 Proteome comparison of HGs between FBs from ITBs and RJBs
    4.6 DISCUSSION
        4.6.1 Proper development and basic functions of the NEBs HGs requires shared proteome
        4.6.2 The NEBs development from ITBs and RJBs adapt divergent HG proteome
        4.6.3 Proper development and basic functions of the NBs HGs requires shared Proteome
        4.6.4 The NBs development from ITBs and RJBs adapt divergent HG proteome
        4.6.5 Proper development and basic functions of the FBs HGs requires shared proteome
        4.6.6 The FBs development from ITBs and RJBs adapt divergent HG proteome
    4.7 CONCLUSION
REFERENCES
ACKNOWLEDGEMENTS
LIST OF TABLES
AUTHOR’S BIOGRAPHY


【参考文献】：
期刊论文
[1]Effect of honey on bacterial translocation and intestinal morphology in obstructive jaundice[J]. Cem Gencay,Kemal Kismet,Bulent Kilicoglu,Serap Erel,Mehmet Ali Akkus,Sibel Serin Kilicoglu,Sabahattin Muratoglu,Asli Elif Sunay,Esra Erdemli.  World Journal of Gastroenterology. 2008(21)



本文编号：3400054