受根腐病致病禾谷镰孢菌侵染后大豆生理生化及分子响应机制研究
发布时间:2021-11-12 11:38
禾谷镰孢菌Fusarium graminearum是近年来引起大豆根腐病的重要致病菌之一,严重影响大豆产量和品质。当前,化学防治病害导致了诸如农药残留、土壤水体污染、病菌抗药性及生态环境破坏等一系列问题。选育和利用抗病品质仍然是防治大豆病害的最经济、有效和环境友好的途径。然而,关于大豆和禾谷镰孢菌的互作机制,特别是大豆对禾谷镰孢菌的抗性机制尚不清楚,有待深入研究。本论文以课题组前期抗性筛选获得禾谷镰孢菌中抗大豆品种南豆12和感病九月黄为材料,采用下胚轴刺伤接种法接种禾谷镰孢菌,于接种后不同时间点检测大豆下胚轴种抗氧化物酶(SOD、POX,GR,CAT和POD)活性、丙二醛(MDA,H2O2含量、活性氧ROS产生情况、大豆异黄酮含量变化,以及抗病相关基因表达情况,以期从生理生化和分子水平解析大豆响应禾谷镰孢菌侵染的机制。主要研究结果如下:禾谷镰孢菌侵染后,三种抗氧化物酶活性和MDA含量均发生变化,其中抗病品种南豆12中CAT和SOD活性较感病品种九月黄高,且MDA含量较九月黄高,而在九月黄中POD活性在病菌侵染早期高于南豆12,后期又降低。受F...
【文章来源】:四川农业大学四川省 211工程院校
【文章页数】:53 页
【学位级别】:硕士
【文章目录】:
摘要
Abstract
Abbreviation
1. Introduction
1.1 The occurrence and harm of soybean root rot
1.2 Pathogen population of soybean root rot is complex
1.3 Fusarium root rot occurs widely in soybean production
1.4 F. graminearum as the important pathogens of soybean root rot
1.5 Effective management of Fusarium related diseases
1.6 Resistance mechanism of soybean to Fusarium graminearum
2. Purpose and Significance of this study
3. Materials and Methods
3.1 Fungal culture
3.2 Soybean germination and fungal inoculation
3.3 Detection of antioxidant enzymes activities
3.4 Detection of ROS (Reactive oxygen species)
3.5 Examination of necrotic cell death
3.6 HPLC Analysis of Isoflavones
3.7 Analysis of defense-related gene expression
3.8 Statistical analysis
4. Results
4.1 Activity of Antioxidant enzyme was induced by F. graminearum but differed in two cultivars
4.2 ROS were produced differently in two soybean cultivars after F. graminearum infection
4.3 Cell death was induced differently in two soybean cultivars after F. graminearum infection
4.4 Four isoflavones were significantly induced by F. graminearum but differed in quantity and change dynamics
4.5 Expression of defense-related genes were induced by F. graminearum
5. Discussion
6. Conclusion
Reference
Dedication
Acknowledgement
Publications list
【参考文献】:
期刊论文
[1]长期连作对大豆根际土壤镰孢菌种群的影响[J]. 魏巍,许艳丽,朱琳,张思佳,李淑娴. 应用生态学报. 2014(02)
[2]东北三省大豆根腐镰孢菌种类及其致病力分析[J]. 王晓艳,文景芝. 中国油料作物学报. 2011(04)
[3]黄淮地区大豆种质资源对疫霉根腐病的抗病性评价[J]. 唐庆华,崔林开,李德龙,戴婷婷,阴伟晓,董莎萌,邢邯,郑小波,王源超. 中国农业科学. 2010(11)
[4]新疆阿勒泰地区大豆镰刀菌根腐病病原鉴定及致病性测定[J]. 白丽艳,张全党,李斌,郭庆元. 新疆农业科学. 2009(03)
[5]不同施肥处理对黑土土壤呼吸的影响[J]. 乔云发,苗淑杰,王树起,韩晓增,李海波. 土壤学报. 2007(06)
本文编号:3490838
【文章来源】:四川农业大学四川省 211工程院校
【文章页数】:53 页
【学位级别】:硕士
【文章目录】:
摘要
Abstract
Abbreviation
1. Introduction
1.1 The occurrence and harm of soybean root rot
1.2 Pathogen population of soybean root rot is complex
1.3 Fusarium root rot occurs widely in soybean production
1.4 F. graminearum as the important pathogens of soybean root rot
1.5 Effective management of Fusarium related diseases
1.6 Resistance mechanism of soybean to Fusarium graminearum
2. Purpose and Significance of this study
3. Materials and Methods
3.1 Fungal culture
3.2 Soybean germination and fungal inoculation
3.3 Detection of antioxidant enzymes activities
3.4 Detection of ROS (Reactive oxygen species)
3.5 Examination of necrotic cell death
3.6 HPLC Analysis of Isoflavones
3.7 Analysis of defense-related gene expression
3.8 Statistical analysis
4. Results
4.1 Activity of Antioxidant enzyme was induced by F. graminearum but differed in two cultivars
4.2 ROS were produced differently in two soybean cultivars after F. graminearum infection
4.3 Cell death was induced differently in two soybean cultivars after F. graminearum infection
4.4 Four isoflavones were significantly induced by F. graminearum but differed in quantity and change dynamics
4.5 Expression of defense-related genes were induced by F. graminearum
5. Discussion
6. Conclusion
Reference
Dedication
Acknowledgement
Publications list
【参考文献】:
期刊论文
[1]长期连作对大豆根际土壤镰孢菌种群的影响[J]. 魏巍,许艳丽,朱琳,张思佳,李淑娴. 应用生态学报. 2014(02)
[2]东北三省大豆根腐镰孢菌种类及其致病力分析[J]. 王晓艳,文景芝. 中国油料作物学报. 2011(04)
[3]黄淮地区大豆种质资源对疫霉根腐病的抗病性评价[J]. 唐庆华,崔林开,李德龙,戴婷婷,阴伟晓,董莎萌,邢邯,郑小波,王源超. 中国农业科学. 2010(11)
[4]新疆阿勒泰地区大豆镰刀菌根腐病病原鉴定及致病性测定[J]. 白丽艳,张全党,李斌,郭庆元. 新疆农业科学. 2009(03)
[5]不同施肥处理对黑土土壤呼吸的影响[J]. 乔云发,苗淑杰,王树起,韩晓增,李海波. 土壤学报. 2007(06)
本文编号:3490838
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