The Cloning,Expression and Function Researches of SERK Genes

发布时间：2022-12-04 11:11

　　Malus hupehensis, common names Chinese crab apple, Hupeh crab or tea crabapple, is a species of flowering plant in the apple genus Malus of the family Rosaceae. It is native to China. Malus hupehensis （Pamp.） Rehd. is naturally distributed in different provinces in China and cultivated as a rootstock and an ornamental plant. Mainly we had used the apomictic plant of ’Pingyi Tiancha’ （Malus hupehensis （Pamp.） Rehd. var. pingyiensis Jiang）,which originates from Pingyi County in the Shandong provin... 

【文章页数】：115 页

【学位级别】：博士

【文章目录】：
Abstract
Chapter 1 Literature review
    1.1 Introduction
    1.2 What is apomixes
    1.3 The potential value of apomixes in agriculture
    1.4 Mechanisms of apomixes
    1.5 Experimental approaches and model systems in apomixis research
    1.6 Role of SERK in plant tissue organogenesis and somatic embryogenesis
    1.7 Bioinformatics analysis of SERKs
    1.8 Uncoupled functions of SERKs in diverse pathways
    1.9 SERK functions in crops
    1.10 Bioinformatics
    1.11 Plant transformation
    1.12 The aim and significance of the research
Chapter 2 Materials and Methods
    2.1 Plant materials
    2.2 Main reagents and important kits
    2.3 Methods
        2.3.1 RNA extraction and cDNA synthesis
        2.3.2 Designing primers
        2.3.3 Rapid amplification of cDNA ends (RACE)
        2.3.4 PCR reaction
        2.3.5 pGM-T cloning
        2.3.6 PCR clean-up system
        2.3.7 Purification plasmid DNA
        2.3.8 Cloning the cDNA full length of SERK2/SERK3
        2.3.9 Sequence alignment and phylogenetic analysis
        2.3.10 Quantitative RT-PCR
        2.3.11 Plant genetic transformation
Chapter 3 Results and Analyses
    3.1 Cloning and structure analysis of SERK2
    3.2 Composition analysis of amino acid of SERK2
    3.3 Sequence characteristics and clustering analysis
    3.4 Cloning of the full-length cDNA of SERK3
    3.5 Hydrophobicity analysis of SERK3
    3.6 Composition analysis of amino acid of SERK3
    3.7 Sequence analysis and bioinformatics of SERK genes
    3.8 Expression analysis
    3.9 Vector construction
    3.10 Molecular identification and statistics of transgenic plants
        3.10.1 Micro-Tom tomato (Solarium esculentum)
        3.10.2 Tobacco (Nicotiana benthamiana)
Chapter 4 Discussions
    4.1 Cloning and sequence analysis of SERKs
    4.2 SERK gene expression analysis
    4.3 Plant transformation
Chapter 5 Conclusions and innovation points
    5.1 Conclusions
    5.2 Innovation points
References
Appendixes
Acknowledgement
Articles published as a candidate for Ph.D


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期刊论文
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