槐米黄酮物质的提取及其抗氧化性研究

发布时间：2021-07-14 00:21

　　槐米是豆科植物国槐的干燥花蕾,广泛栽培于中国的各个省市、日本和韩国。槐米是一种珍贵的中草药,具有清热、凉血和止血作用。本试验以产自山西省夏县的槐米为试验原料,研究了槐米黄酮类化合物的提取工艺,槐米中多种黄酮物质的测定方法及其抗氧化性能。本文研究结果如下:本研究建立了定性和定量分析槐米提取物中六种黄酮化合物的高效液相色谱方法。实验条件为:流动相由0.1%磷酸水溶液（A）和乙腈（B）组成。使用前用45μm微孔滤膜过滤,并超声脱气。色谱柱Diamonsil TMC18柱（250mm×4.6mm,5μm）,柱温为30℃,进样量为5μL,流速为1.0 mL/min。目标组分的检测波长为360 nm。流动相梯度洗脱条件如下:0-30 min,95-65%A;30-35min,65-95%A;35-50 min,95%A。结果表明该方法准确、有效,可用于槐米黄酮物质的质量控制。采用超声辅助提取槐米中的芦丁、烟草苷、水仙苷、山奈酚、异鼠李素、槲皮素和总黄酮。通过单因素试验和响应面法确定了最佳提取条件为:乙醇浓度为70%,时间30 min,温度61℃,液固比15.30mL/g。芦丁、烟草苷、水仙苷、山奈... 

【文章来源】：山西大学山西省

【文章页数】：93 页

【学位级别】：硕士

【文章目录】：
摘要
Abstract
Chapter 1 Review of the literature
    1.1 The overview of Flos Sophorae Immaturus
        1.1.1 The profile of Flos Sophorae Immaturus
        1.1.2 Nutrient composition analysis of FSI
        1.1.3 The utilization value of FSI
    1.2 Overview of flavonoids from FSI
        1.2.1 Pharmacology activities
        1.2.2 Determination of flavonoids from FSI
        1.2.3 Extraction methods of flavonoids from FSI
        1.2.4 Purification of flavonoids from FSI
    1.3 Research content, research purpose and research significance
        1.3.1 Research content
        1.3.2 Research purpose
        1.3.3 Research significance
Chapter 2 Determination of the six flavonoids in Flos Sophorae Immaturus byHPLC
    2.1 Introduction
    2.2 Materials and Methods
        2.2.1 Materials and reagents
        2.2.2 HPLC conditions
        2.2.3 Preparation of standard stock solution
        2.2.4 Preparation of sample solution
        2.2.5 Validation
        2.2.6 Statistical analysis
    2.3 Results and discussion
        2.3.1 Chromatographic profile
        2.3.2 Validation of the HPLC method for FSI extract
        2.3.3 Specificity/Selectivity
        2.3.4 Linearity, LOD and LOQ
        2.3.5 Precision
        2.3.6 Stability
        2.3.7 Repeatability
        2.3.8 Accuracy/Recovery assay
        2.3.9 Sample determination
    2.4 Summary
Chapter 3 Optimization of Ultrasound-Assisted Extraction for SimultaneousQuantitation of Six Flavonoids in Flos Sophorae Immaturus
    3.1 Introduction
    3.2 Materials and methods
        3.2.1 Sample of FSI
        3.2.2 Chemicals and Apparatus
        3.2.3 Preparation of Standard Solution
        3.2.4 Determination of TFFSI by UV
        3.2.5 Determination of six main flavonoids by HPLC
        3.2.6 Sample Preparation Based on UAE
        3.2.7 Experimental Design
        3.2.8 Statistical analysis
    3.3 Results and discussion
        3.3.1 Univariate Experiments Design and Analysis
        3.3.2 Multi-Response Design and Analysis
    3.4 Summary
Chapter 4 Antioxidant activity of Flavonoids in Flos Sophorae Immaturus
    4.1 Introduction
    4.2 Materials and Methods
        4.2.1 Chemicals and Apparatus
        4.2.2 Sample Preparation
        4.2.3 Measurement of scavenging activity on DPPH radicals
        4.2.4 Measurement of scavenging activity on ABTS·+
        4.2.5 Superoxide anion scavenging activity (SASA)
        4.2.6 Ferric reducing antioxidant power (FRAP)
        4.2.7 Statistical analysis
    4.3 Results and discussion
        4.3.1 Scavenging activity of DPPH radical
        4.3.2 Scavenging activity of ABTS·+
        4.3.3 Scavenging activity of Superoxide anion (·O_2~-) radical
        4.3.4 Ferric reducing antioxidant power (FRAP)
    4.4 Summary
Chapter 5 Conclusion and Prospect
    5.1 Conclusion
    5.2 Prospect
References
攻读学位期间取得的研究成果
致谢
个人简况及联系方式



本文编号：3283015