电致化学发光分子印迹传感器的制备及其在毒品检测中的应用

发布时间：2018-01-11 13:27

本文关键词：电致化学发光分子印迹传感器的制备及其在毒品检测中的应用　出处：《江南大学》2012年硕士论文　论文类型：学位论文

【摘要】：毒品泛滥已成全球的一大公害,每年约20万人因滥用毒品致死,上千万人丧失劳动力,严重破坏了社会发展与稳定。如何在飞机、火车、海关等要地快速灵敏的识别出不法分子藏匿于行李、邮件的毒品,成为缉毒部门的一项重要任务。目前,对毒品的检测方法有很多。但这些方法普遍存在仪器昂贵、样品前处理过程复杂、检测时间长等缺点,尤其是检测限偏高,选择性不好,对痕量毒品的检测不够灵敏。固相电致化学发光具有试剂用量少,灵敏度高等优点,分子印迹技术具有良好的识别能力和富集能力。本文研究了将这两种方法联用的电致化学发光-分子印迹传感器。具体内容包括: 1.将电致化学发光检测手段与分子印迹技术相结合,制备了一种高灵敏度、高选择性的检测甲基苯丙胺的电化学发光-分子印迹传感器。首先在玻碳电极表面修饰发光配合物Ru(bpy)32+/Nafion/MWCNT得到电化学发光固相电极,然后在其基础上制备对甲基苯丙胺具有特异性识别功能的sol-gel印迹膜。通过循环伏安,电化学发光,扫描电镜等手段对传感器性能进行了表征。该传感器对目标分子具有高灵敏度和选择性,线性范围1.0×10~(-9) ~ 1.0×10~(-12) mol/L,检测限可达1.0×10~(-15) mol/L。能够检测出密闭容器中挥发出来的痕量甲基苯丙胺。 2.制备了一种测定吗啡的新型ECL-MIP传感器,首先基于MWCNT/Titania /Nafion复合膜将联吡啶钌固定在电极表面,得到固相发光电极,然后再修饰一层选择性印迹膜,该传感器可对溶液中的吗啡分子进行特异性识别和富集,具有很高的检测灵敏度和优异的选择性,线性范围1.0×10~(-6) ~ 1.0×10~(-9) mol/L,检测限可达2.0×10~(-10) mol/L。并且该传感器具有良好的稳定性及再生能力,有望成为一种灵敏快速、选择性高、再生性好的吗啡分析新方法。 3.采用一种新型金属铱配合物(ppy)_2Ir(N-phBA)(其中ppy = 2-苯基吡啶,N-phBA = N-苯基苯酰胺)作为发光试剂,以三丙胺为共反应物,探讨了其在乙腈溶液中的电化学及电致化学发光行为。制备了多壁碳纳米管/铱(III)修饰电极,对其电致发光性能及稳定性进行考察。结果表明,(ppy)_2Ir(N-phBA)是一种优良的电致化学发光试剂,发光强度较大且背景发光低;多壁碳纳米管/铱(III)修饰电极制作简单,容易保存,稳定性好,可望用于含氮有机物的在线快速测定,在电致化学发光领域具有潜在的分析应用价值。
[Abstract]:Drug abuse has become a major public hazard in the world, about 200,000 people die of drug abuse every year, tens of millions of people have lost their labor force, which has seriously damaged social development and stability. How to fly and train. Customs and other important places to quickly and sensitively identify illegal elements hiding in luggage, mail drugs, has become an important drug department task. There are many methods for the detection of drugs, but these methods have many disadvantages, such as expensive instruments, complex sample pretreatment process, long detection time, especially high detection limit and poor selectivity. The detection of trace drugs is not sensitive enough. Solid-phase electrochemiluminescence has the advantages of less reagent dosage and high sensitivity. Molecular imprinting technology has good recognition ability and enrichment ability. In this paper, electrochemiluminescence and molecular imprinting sensors combined with these two methods are studied. The main contents are as follows: 1. A kind of high sensitivity was prepared by combining electrochemiluminescence with molecular imprinting. A highly selective electrochemiluminescence sensor for the detection of methamphetamine-molecularly imprinted sensor. The first step is to modify the luminescent complex Runbpyy on the surface of glassy carbon electrode. 32 / Nafion / MWCNT electroluminescent solid state electrode was obtained. Then the sol-gel imprinted membrane with specific recognition function for methamphetamine was prepared on the basis of the membrane. The electrochemiluminescence (ECL) was obtained by cyclic voltammetry. The performance of the sensor was characterized by scanning electron microscopy. The sensor has high sensitivity and selectivity to the target molecule. The linear range is 1.0 脳 10 ~ (-9) ~ 1.0 脳 10 ~ (10 ~ (-12)) mol/L. The detection limit is up to 1.0 脳 10 ~ (-1) -15) mol 路L ~ (-1) 路L ~ (-1). Trace methamphetamine volatilized in a closed vessel can be detected. 2. A novel ECL-MIP sensor for the determination of morphine was prepared. Firstly, bipyridine ruthenium was immobilized on the electrode surface based on MWCNT/Titania / Nafion composite membrane. Solid-phase luminescent electrode was obtained and then modified with a selective imprinted membrane. The sensor can specifically identify and enrich morphine molecules in the solution with high detection sensitivity and excellent selectivity. The linear range is 1.0 脳 10 ~ (-6) ~ 1.0 脳 10 ~ (10 ~ (-9)) mol/L. The detection limit is up to 2.0 脳 10 ~ (-10) mol 路L ~ (-1) mol 路L ~ (-1). The sensor has good stability and regeneration ability, so it is expected to be a sensitive, rapid and highly selective sensor. A new method for morphine analysis with good reproducibility. 3. A new metal iridium complex (ppy = 2-phenylpyridine) was used. N-phBA = N-phenylbenzamide was used as luminescent reagent and tripropylamine was used as co-reactant. The electrochemistry and electrochemiluminescence behavior of multiwalled carbon nanotube / iridium iridium II modified electrode in acetonitrile solution were investigated. The electroluminescent properties and stability of the electrode were investigated. It is a kind of excellent electrochemiluminescence reagent with high luminescence intensity and low background luminescence. The multiwalled carbon nanotube / iridium iridium iridium modified electrode is simple, easy to preserve and stable. It is expected to be used in the on-line rapid determination of nitrogen-containing organic compounds and has potential application value in electrochemiluminescence.
【学位授予单位】：江南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：D919.1;TP212

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