人指纹的STR分型检测及法医学应用研究

发布时间：2018-02-25 18:07

本文关键词： 汗潜指纹血潜指纹低拷贝模板(LCN) 指纹显现磁珠法硅珠法 microcon-100 聚合酶链反应　出处：《昆明医学院》2006年硕士论文　论文类型：学位论文

【摘要】：目的建立渗透性与非渗透性载体上显现与未显现的汗潜指纹、血潜指纹的DNA检验方法，最大限度地发挥指纹特别是残缺不全以及模糊不清指纹的证据作用，为侦查破案及诉讼提供科学依据。方法采用Chelex-100法与Microcon-100纯化浓缩相结合提取法、磁珠法和Invisorb spin forensic试剂盒提取纯化人指纹中DNA，低拷贝模板(LCN)STR复合扩增、荧光电泳检测。结果首次建立了用Invisorb spin forensic试剂盒及QIAGENE系统对汗潜指纹DNA的提取纯化方法。玻片、纸张、胶带纸上的汗潜指纹均可得到完整的power plex16基因座的STR分型。对各种载体上经过常用的指纹显现法显现的汗潜指纹进行了系统研究。结果表明用铜粉、铝粉、荧光粉、黑磁粉、“502”胶、茚三酮、磺酸双三嗪荧光显色液显现的玻片和纸张上汗潜指纹可成功进行STR分型，而硝酸银显现的指纹不能成功进行STR基因分型，另外四甲基联苯胺、茚三酮、铝粉和铜粉显现后1小时、2周及1月提取的血潜指纹DNA，STR基因分型结果无明显影响，，而氨基黑10B对DNA分型有一定影响。结论建立了汗潜指纹、血潜指纹显现前后的微量DNA检验方法，并成功应用于实际案例。课题研究所创建的方法简单新颖、结果灵敏、稳定、可重复性好。
[Abstract]:Objective to establish a DNA method for detecting perspiration latent fingerprints and blood latent fingerprints on permeable and non-permeable carriers, so as to give full play to the evidence function of fingerprints, especially incomplete fingerprints and fuzzy fingerprints. Methods Chelex-100 method combined with Microcon-100 purification and concentration extraction method, magnetic bead method and Invisorb spin forensic kit were used to extract and purify DNA from human fingerprint. Results the method of extracting and purifying sweat latent fingerprint DNA by Invisorb spin forensic kit and QIAGENE system was established for the first time. The sweat latent fingerprint on the tape paper can obtain the complete STR typing of the power plex16 locus. The sweat latent fingerprint displayed by the common fingerprint display method on various carriers was studied systematically. The results showed that copper powder, aluminum powder and phosphor were used. Black magnetic powder, "502" gel, indene trione, ditriazine sulfonic acid fluorescent chromogenic liquid glass slide and paper sweat latent fingerprint can be successfully classified by STR, but silver nitrate fingerprint can not successfully carry out STR genotyping, and tetramethyl benzidine, indene trione, The results of DNA STR genotyping from blood latent fingerprints extracted from aluminum powder and copper powder at 1 hour and 2 weeks after appearance and on January had no significant effect, but amino-black 10B had some influence on DNA typing. Conclusion the sweating latent fingerprint was established. The trace DNA method before and after the development of blood latent fingerprints was successfully applied to practical cases. The method established in this paper is simple and novel, the results are sensitive, stable and reproducible.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919

【引证文献】