VEGF和cTnT在家兔缺血心肌中的表达及其死后稳定性研究

发布时间：2018-03-07 22:39

本文选题：法医病理学　切入点：早期心肌梗死　出处：《重庆医科大学》2006年硕士论文　论文类型：学位论文

【摘要】： 背景:SCD的研究是法医学最重要的任务之一,而EMI的死后诊断是其重点和难点。不足6h的心肌梗死,在肉眼和显微镜下均无特异性改变;不使用特殊的方法,是不可能作出EMI的组织学诊断并把其作为猝死原因的。在常规HE染色出现特征性形态学改变以前,缺血心肌已发生了一系列病理生理变化。生物化学方法,组织化学、酶组织化学、荧光组织化学染色,电子显微镜技术,能够显示早期心肌损害。但是上述方法或因操作过于复杂而不利于常规使用,或因缺乏特异性、以及易受组织自溶、腐败等因素的影响,而限制了它们在法医学实践中的应用。此前有研究表明,cTnT和VEGF的IHC染色是显示心肌缺氧可靠和敏感的方法,可望用于由心肌缺血引起的SCD的死后诊断。用于EMI死后诊断的IHC指标能否应用于法医学实践,除了需要考虑其特异性和敏感性外,还必须考虑组织自溶、腐败等因素对抗原的影响。目前,用于EMI死后诊断的各种IHC指标的死后稳定性及其在死后不同时间的表达变化规律是值得进一步研究的重点。目的:本研究用冠状动脉左前降支结扎的方法建立家兔急性心肌缺血模型,检测心肌中VEGF和cTnT两项免疫组化指标在死后不同时间的表达情况,旨在探寻其死后稳定性及表达的变化规律,以期对其在法医学实践中诊断EMI的应用价值进行评价。方法:选取健康成年新西兰兔12只,雌雄不限,体重2500g—3000g,随机分为对照组和实验组。对照组兔直接处死,取左心室心肌;
[Abstract]:Background: the study of EMI is one of the most important forensic studies of Ren Wuzhi, and the postmortem diagnosis of EMI is its key and difficult point. After less than 6 hours of myocardial infarction, there is no specific change under the naked eye and microscope; no special methods are used. It is impossible to make a histologic diagnosis of EMI and use it as a cause of sudden death. Before the characteristic morphological changes in routine HE staining, a series of pathophysiological changes had taken place in ischemic myocardium. Enzyme histochemistry, fluorescent histochemical staining, electron microscopy can show early myocardial damage. But these methods are either too complex to be used routinely, or because of lack of specificity and vulnerability to tissue autolysis, The influence of factors such as corruption limits their application in forensic practice. Previous studies have shown that IHC staining of cTnT and VEGF is a reliable and sensitive method for showing myocardial hypoxia. It is expected to be used in the postmortem diagnosis of SCD caused by myocardial ischemia. Whether the IHC markers used in the postmortem diagnosis of EMI can be applied to forensic practice should not only be considered for its specificity and sensitivity, but also for tissue autolysis. At present, the postmortem stability of various IHC markers used for postmortem diagnosis of EMI and their expression changes at different postmortem times are worthy of further study. Objective: to establish a rabbit model of acute myocardial ischemia by ligating the left anterior descending coronary artery, and to detect the expression of VEGF and cTnT in myocardium at different time after death. The purpose of this study was to explore the changes of postmortem stability and expression of EMI and to evaluate its application in forensic practice. Methods: twelve healthy adult New Zealand rabbits, male and female, weighing 2500g-3000g, were randomly divided into two groups: the control group and the experimental group. The rabbits in the control group were killed directly and the left ventricular myocardium was taken.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919

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