输血对受者血液循环中STRs基因型影响的研究

发布时间：2018-03-09 19:40

  本文选题：输血　切入点：STR基因座　出处：《四川大学》2005年硕士论文　论文类型：学位论文

【摘要】：目的 探讨不同输血量、输血后不同时间供者STR_s基因型对受者SWR_s基因型检测结果是否有影响。方法3例患者分别接受输血400ml、800ml及1200ml,输血前以及输血后分别在4h、8h、12h采集受者血液,受者和供者血样用EDTA抗凝,采用Chelex-100法提取DNA,—4℃保存备用。选择D1S549,D18S865,D3S1754,D12S391,D12S375,D6S477等6个基因座进行PCR扩增,电泳分型。结果3例患者分别输血400ml、800ml及1200ml后,输血前及输血后12h内采集血样检测的6个基因座STRs基因型完全一致。结论 本次研究结果表明,输血量在1200ml以下的受者输血后12h内STR_s基因型检测结果未受供者血液STR_s基因型的干扰。
[Abstract]:Objective to investigate the effect of STR_s genotypes on the detection of SWR_s genotypes in recipients with different blood transfusions and donor genotypes at different time after transfusion. Methods three patients received 400ml and 1200ml of blood transfusion respectively. The blood samples were collected from the recipients before and after blood transfusion for 8 h and 12 h, respectively. The recipient and donor blood samples were extracted and stored at 4 鈩，

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