Caspase-7在大鼠颅脑损伤后表达的免疫组织化学研究

发布时间：2018-03-10 23:20

本文选题：法医学　切入点：caspase-7　出处：《重庆医科大学》2006年硕士论文　论文类型：学位论文

【摘要】： 目的:创伤性颅脑损伤是法医工作中经常见到的一种损伤,脑损伤后引起的神经细胞死亡通过坏死和凋亡两种途径实现。凋亡发生是一个复杂的,由caspase家族成员介导的蛋白酶级联反应过程。Caspase-7是caspase级联反应中的下游分子之一,为由303个氨基酸组成的蛋白质,分子量为35kD,存在于多种细胞系,弥散分布在胞浆内和近膜结构上,是凋亡的最终效应分子之一。正常情况下Caspase-7以酶原的形式存在,在凋亡过程中被其上游分子活化裂解为两个活性亚单位,作用于底物,使凋亡得以执行。早期认为Caspase-7在中枢神经系统凋亡中并无重要作用,但近年的研究提示其似乎在神经细胞的凋亡中扮演重要的角色,故其日益受到关注。本文采用自由落体撞击模型和SABC法研究大鼠颅脑损伤后caspase-7的IHC表达情况,旨在发现其表达是否具有规律性,希望能为法医学颅脑损伤的损伤时间推断补充新的指标。方法:采用完全随机分组方法,根据造创后处死时间不同将实验大鼠随机分为伤后1h、3h、6h、12h、1d、3d、5d、7d、10d、14d组,另设正常对照组及假手术组。在预定时间分别处死大鼠,取损伤处及其周围区域(对照组、假手术组取与实验组相同的部位),进行HE染色和caspase-7免疫组化SABC法染色,并对染色结果进行光镜观察、图像分析及统计学处理,以了解caspase-7在大鼠颅脑损伤后的表达情况和规律。
[Abstract]:Objective: traumatic craniocerebral injury is a kind of injury that is often seen in forensic medicine. The death of nerve cells caused by brain injury is realized by two ways: necrosis and apoptosis. The protease cascade process mediated by members of the caspase family. Caspase-7 is one of the downstream molecules in the caspase cascade reaction. It is a 303-amino acid protein with a molecular weight of 35kD.It exists in a variety of cell lines and distributes in the cytoplasm and near membrane structure. Caspase-7 is one of the final effector molecules of apoptosis. Normally, Caspase-7 exists in the form of prozyme and is activated into two active subunits by upstream molecules during apoptosis, acting on the substrate. It is believed that Caspase-7 does not play an important role in central nervous system apoptosis, but recent studies suggest that Caspase-7 may play an important role in neuronal apoptosis. Therefore, it has attracted more and more attention. In this paper, the expression of caspase-7 IHC in rats after craniocerebral injury was studied by free fall impact model and SABC method, in order to find out whether the expression of caspase-7 was regular or not. It is hoped that it can be used to infer the injury time of forensic craniocerebral injury. Methods: the experimental rats were randomly divided into two groups according to the time of death after injury: the rats were randomly divided into three groups: 1 hour after injury, 3 hours after injury, 1 hour after injury, 3 days after injury, 3 days after injury, 5 days after injury, 7 days after 7 days and 10 days after 14 days, and then set up normal control group and sham operation group, and the rats were killed at the scheduled time, and the rats were divided into two groups: the control group and the sham operation group. The site of injury and its surrounding area (control group, sham-operation group and experimental group) were taken for HE staining and caspase-7 immunohistochemical SABC staining. The staining results were observed by light microscopy, image analysis and statistical analysis. To understand the expression and regularity of caspase-7 after craniocerebral injury in rats.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919

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