微测序法检测Y-SNP和mt-SNP的研究

发布时间：2018-03-19 04:28

本文选题：Y染色体　切入点：线粒体　出处：《四川大学》2006年博士论文　论文类型：学位论文

【摘要】：目的单核苷酸多态性(single nucleotide polymorphism SNP)是人类基因组中最常见的一种变异，其中Y染色体非重组区及线粒体上SNP在遗传过程中不发生重组，分别呈父系和母系遗传，因而建立高通量的检测方法检测SNP是对现有法医学STR检测手段的必要补充。本课题旨在建立一种复合Y-SNP和mt-SNP位点的快速检测方法，通过群体遗传学研究获得四川地区人群12个Y-SNP及7个mt-SNP位点遗传学资料，，并探讨其在法医学案件检验中的应用。方法采用商品试剂SNaPshot对SNP位点进行复合PCR和复合微测序检测分析。选择了文献资料中曾在其他种族中报道过的SRY2627、SRY1532、M13、M20、SRY8299、Tat、M69、M9、92R7、M112、M17、M19等12个Y-SNPs位点和4216、7028、10398、10400、12308、12705、14766等7个mt-SNP进行研究。具体方法是：(1)对
[Abstract]:Objective single nucleotide polymorphism single nucleotide polymorphism (SNPs) is the most common mutation in the human genome, in which SNP in the unrecombination region of Y chromosome and mitochondria do not recombine during the genetic process, but are patrilineal and patrilineal, respectively. Therefore, the establishment of high-throughput detection method for SNP is a necessary supplement to the existing forensic STR detection methods. This paper aims to establish a rapid detection method for compound Y-SNP and mt-SNP loci. The genetic data of 12 Y-SNP and 7 mt-SNP loci in Sichuan population were obtained and their application in forensic examination was discussed. Methods A commercial reagent SNaPshot was used to detect SNP loci by compound PCR and composite microsequencing. Seven mt-SNP sites, including 12 Y-SNPs loci, including 12 Y-SNPs loci, including 12 Y-SNPs loci, such as M69M9, M9R7, M112M17M19, and 7 mt-SNP sites, such as SRY2627, SRY1532, M13, M20, and so on, were selected, which were previously reported in other ethnic groups. The specific method was to study the seven mt-SNP loci, such as M69M9, M9, M9R7, M12, M17M19, and 42216C7028, 10398FU, 1030810705, 1270566, etc. The specific methods were as follows:
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：D919

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