EB病毒重组Rta蛋白多克隆抗体的初步应用

发布时间：2018-03-29 21:15

本文选题：EB病毒　切入点：Rta　出处：《四川大学》2007年硕士论文

【摘要】： 背景EB病毒是被明确列为致癌因子的一种γ疱疹病毒，它与鼻咽癌、B细胞淋巴瘤、霍奇金氏病、伯基特淋巴瘤等多种恶性肿瘤密切相关。Rta蛋白是EB病毒裂解期立即早期基因之一BRLF1的编码产物，是EB病毒由潜伏期转向裂解复制的关键调控因子。它能引起一系列裂解早期基因的“瀑布式表达”，最终引发EB病毒裂解传染。目前Rta蛋白已成为国外研究的重点。目的以特异性的抗Rta多克隆抗体体作为探针，初步研究Rta在胃癌(gastric carcinoma)、食管癌(esophageal carcinoma)、乳腺癌(breast carcinoma)和肺癌(lung carcinoma)中的表达情况。方法采用免疫组织化学的方法(Envision二步法)，将纯化好的多克隆抗体作为一抗，检测了45例食管癌、30例胃癌、42例乳腺癌和32例肺癌组织样本中Rta蛋白的表达情况。结果45例食管癌中显示对抗Rta抗体反应阳性者6例，相应癌旁组织均为阴性，P=0.026；30例胃癌中显示对抗Rta抗体反应阳性者6例，相应癌旁组织均为阴性，P=0.031；42例乳腺癌中显示对抗Rta抗体反应阳性者7例，相应癌旁组织均为阴性，，P=0.012；32例肺癌中显示对抗Rta抗体反应阳性者4例，相应癌旁组织均为阴性，P=0.113。结论EB病毒的裂解感染状态与食管癌、胃癌和乳腺癌的发生具有一定相关性，裂解感染期EBV可能在上述恶性肿瘤的发病中起了一定作用。
[Abstract]:The background of EB virus is specified as a gamma herpesvirus carcinogen, and nasopharyngeal carcinoma, B cell lymphoma, Hodge's disease, Burkitt's lymphoma and other malignant tumors is closely related to.Rta protein of EB virus is cracking immediately one of the early gene BRLF1 encoding product, EB is a key regulator of virus from latency to lytic replication. It can cause a series of early cracking "waterfall gene expression", eventually lead to cracking of EB virus infection. The Rta protein has become the focus of research.
Objective to investigate the expression of Rta in gastric cancer (gastric carcinoma), esophageal cancer (esophageal carcinoma), breast cancer (breast carcinoma) and lung cancer (lung carcinoma) by using the specific anti Rta polyclonal antibody as a probe.
Methods using immunohistochemical method (Envision two step method), the purified polyclonal antibody was used as an antibody to detect the expression of Rta protein in 45 cases of esophageal cancer, 30 cases of gastric cancer, 42 cases of breast cancer and 32 cases of lung cancer.
Results 45 cases of esophageal carcinoma showed anti Rta antibody positive in 6 cases, paracancerous tissues were negative for P=0.026; in 30 cases of gastric carcinoma showed anti Rta antibody positive in 6 cases, paracancerous tissues were negative for P=0.031; in 42 cases of breast cancer showed anti Rta antibody positive in 7 cases and the corresponding paracancerous tissues were negative for P=0.012; 32 cases of lung cancer showed anti Rta antibody positive in 4 cases, paracancerous tissues were negative for P=0.113.
Conclusion there is a certain correlation between the infection status of EB virus and the occurrence of esophageal cancer, gastric cancer and breast cancer. EBV may play a role in the pathogenesis of these malignant tumors.

【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：D919

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