大鼠机械性损伤后皮下肌肉出血的病理学观察

发布时间：2018-04-24 07:30

本文选题：法医病理学 + 皮下肌肉出血　；参考：《中国法医学杂志》2011年05期

【摘要】：目的观察生前机械性损伤后不同时间皮下肌肉出血的病理形态学变化。方法 56只SD大鼠随机分成生前损伤组(48只)、死后损伤组(4只)和正常对照组(4只);生前损伤组按不同时间再分为12组,建立机械性损伤模型;用HE及普鲁士蓝染色法观察皮下肌肉出血的病理形态学变化。结果 HE染色:生前损伤6～24h,损伤区见较多红细胞及炎细胞浸润,以中性粒细胞为主,少量单核细胞及淋巴细胞,未见棕黄色颗粒;2～3d,红细胞开始减少,炎细胞浸润达到高峰,而后开始减少;3～5d,肉芽组织开始增生,并可见极少量棕黄色颗粒;6～10d,肉芽组织开始机化,成纤维细胞及胶原纤维数量开始增多,并可见少量棕黄色颗粒;10～16d,纤维组织形成。普鲁士蓝染色:3d,见极少量含铁血黄素蓝染颗粒,并随时间推移逐渐增多,6～10d达到高峰1,0～16d后逐渐减少至消失。死后损伤组及正常对照组均无上述改变。结论通过HE及普鲁士蓝染色法观察皮下肌肉出血的病理形态学变化,有助于对机械性损伤出血时间的推断。
[Abstract]:Objective to observe the pathomorphological changes of subcutaneous muscle hemorrhage after mechanical injury. Methods Fifty-six Sprague-Dawley rats were randomly divided into antemortem injury group (n = 48) and postmortem injury group (n = 4) and normal control group (n = 4). The histopathologic changes of subcutaneous muscle hemorrhage were observed by HE and Prussian blue staining. Results HE staining showed that there were more red blood cells and inflammatory cells infiltrating in the injured area, mainly neutrophils, a few monocytes and lymphocytes, and no brown granules for 3 days. The red blood cells began to decrease and the inflammatory cell infiltration reached the peak. Then the granulation tissue began to proliferate for 5 days, and a very small amount of brown granules began to proliferate. The granulation tissue began to be organized, the number of fibroblasts and collagen fibers began to increase, and a small amount of brown granules began to proliferate for 16 days, and the fibrous tissue was formed. When Prussian blue was dyed for 3: d, a very small amount of iron containing lutein blue granules were found, and gradually increased with time, reaching a peak of 10 days, reaching a peak of 10, 0, 16 days, and then gradually decreasing to disappear. No such changes were found in postmortem injury group and normal control group. Conclusion to observe the pathomorphological changes of subcutaneous muscle hemorrhage by HE and Prussian blue staining is helpful to infer the bleeding time of mechanical injury.
【作者单位】：温州医学院法医学教研室;温州医学院基础医学院病理形态中心;
【基金】：2009浙江省“新苗人才计划”资助项目(2009R413020)
【分类号】：D919

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