组织细胞DNA降解与死亡时间的相关性的显微拉曼光谱分析

发布时间：2018-04-28 16:57

本文选题：显微拉曼光谱 + DNA降解　；参考：《光谱学与光谱分析》2010年06期

【摘要】：为了给死亡时间推断的研究提供新的途径与数据,探讨了人死亡后组织细胞DNA含量变化与死后时间间隔关系。将离体肾、肝组织置于特定环境中,在不同时间点提取离体组织,利用激光共焦显微拉曼光谱技术和统计学方法,在拉曼位移测试范围内,对死后不同时间肾、肝组织DNA含量变化进行拉曼光谱检测与统计学分析。结果显示:人死亡后48～72h,随着死亡时间的推移,肾、肝组织Raman光谱的主要散射峰峰位没有明显变化,而其峰高有明显差异;所测组织的激光拉曼光谱特征峰的相对峰强值(I1094/I2923)随死亡时间的推移而减小。因此可以得出结论:人死亡后肾、肝组织细胞DNA含量随死亡时间延长呈下降趋势,两者之间存在线性关系。
[Abstract]:In order to provide a new approach and data for the estimation of death time, the relationship between the changes of DNA content and postmortem interval was studied. The isolated kidney and liver tissue were placed in a specific environment and extracted at different time points. Laser confocal microscopy Raman spectroscopy and statistical method were used to measure the Raman displacement of the kidney at different time after death. The changes of DNA content in liver tissue were detected by Raman spectroscopy and analyzed statistically. The results showed that with the passage of death time, the main scattering peaks of Raman in kidney and liver had no obvious change, but the peak height was different. The relative peak intensity of the characteristic laser Raman spectra of the tissue I _ 1094 / I _ 2923) decreases with the time of death. Therefore, it can be concluded that the content of DNA in kidney and liver tissue cells decreases with the prolongation of death time after death, and there is a linear relationship between them.
【作者单位】：南华大学医学院法医教研室;南华大学数理学院;
【基金】：国家自然科学基金项目(30371626) 湖南省科技厅项目(2008FJ3084)资助
【分类号】：D919

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