弓形虫感染对小鼠脑内c-fos及ACTH表达的影响

发布时间：2018-05-01 23:32

本文选题：弓形虫 + c-fos　；参考：《吉林大学》2007年硕士论文

【摘要】： 本文通过尾静脉注射方式使KM小鼠感染弓形虫,观察大脑皮质、脑干、间脑、中脑及海马组织中弓形虫的动态分布,同时,应用免疫组织化学技术观察原癌基因c-fos及ACTH在脑组织中的表达。探讨在神经内分泌免疫网络中:肾上腺皮质激素(ACTH)和c-fos在弓形虫病脑组织中的表达及其意义。实验结果表明:小鼠尾静脉感染弓形虫的第8天,大脑皮质和间脑中观察到弓形虫滋养体;第10天,在脑干、中脑及海马中查获虫体。②免疫组织化学染色检测显示:脑组织内ACTH表达明显,呈阳性反应的ACTH胞体出现在大脑皮质及下丘脑区内,ACTH免疫反应阳性纤维出现在下丘脑及胼胝体内,大量而密集的ACTH免疫反应阳性纤维主要集中在下丘脑第三脑室室周区、室旁核、弓状核及其相邻外侧区域内。③在弓形虫感染小鼠的大脑脑组织内有c-fos的表达,但与对照组比较,出现明显差异,大脑皮质神经元胞体内出现c-fos免疫反应阳性颗粒,染色强度明显低于对照组。海马的神经元胞体内未见有c-fos免疫反应阳性颗粒。下丘脑神经元胞体内则出现含量较多的免疫反应阳性颗粒,且显色强度高于对照组,这些免疫反应阳性颗粒主要集中于大神经细胞内。结论:①弓形虫(RH株)静脉途径感染小鼠,虫体通过血脑屏障侵入脑组织;脑组织区域不同,血脑屏障对弓形虫的阻隔作用存在差异。②感染弓形虫小鼠脑中,大脑皮质和下丘脑的神经元中ACTH的表达明显增强。③感染弓形虫小鼠,在海马和大脑皮质c-fos蛋白表达较正常组显著降低,而在下丘脑区c-fos蛋白表达则明显高于正常对照组。
[Abstract]:In this paper, km mice were infected with Toxoplasma gondii by tail vein injection, and the dynamic distribution of Toxoplasma gondii in cerebral cortex, brainstem, diencephalon, midbrain and hippocampus was observed. The expression of proto-oncogene c-fos and ACTH in brain tissue was observed by immunohistochemical technique. To investigate the expression and significance of ACTH and c-fos in neuroendocrine and immune networks in Toxoplasma gondii. The results showed that Toxoplasma gondii trophozoites were observed in the cerebral cortex and diencephalon on the 8th day of Toxoplasma gondii infection in the tail vein of mice, and in the brain stem on the 10th day. Immunohistochemical staining showed that the expression of ACTH was obvious in the brain and hippocampus. The positive ACTH cells appeared in the hypothalamus and corpus callosum, and a large number of ACTH immunoreactive fibers were concentrated in the periventricular area of the hypothalamus. The expression of c-fos in paraventricular nucleus, arcuate nucleus and adjacent lateral region was found in the brain tissue of mice infected with Toxoplasma gondii, but there was significant difference between the control group and the control group. C-fos immunoreactive granules appeared in the cortical neurons. The staining intensity was obviously lower than that of the control group. No c-fos immunoreactive granules were found in the neuronal bodies of hippocampus. There were more immunoreactive granules in hypothalamic neurons, and the color intensity was higher than that in the control group. These immunoreactive granules were mainly concentrated in large nerve cells. Conclusion the mice infected with Toxoplasma gondii (RH strain) via venous pathway, the parasites invade into the brain tissue through blood-brain barrier, and the barrier effect of blood-brain barrier to Toxoplasma gondii is different in mice with different brain tissue regions. 2. Infection of Toxoplasma gondii in mice with Toxoplasma gondii. The expression of ACTH in neurons of cerebral cortex and hypothalamus was significantly increased in mice infected with Toxoplasma gondii. The expression of c-fos protein in hippocampus and cerebral cortex was significantly lower than that in normal group, but the expression of c-fos protein in hypothalamus was significantly higher than that in normal control group.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：D919

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