RHCE基因分型的实验性研究

发布时间：2018-05-14 19:33

本文选题：Rh血型 + RHCE基因　；参考：《中国医科大学》2005年硕士论文

【摘要】：前言 Rh血型系统是最为复杂、最具多态性的人类血型系统之一,具有重要的临床意义。同时,作为一种优良的遗传标记,Rh血型从发现的时候开始就被广泛应用于法医学实践。目前中国的Rh血型分型方法是传统的血清学技术,这种技术在实际应用中存在着种种弊端。比如:实验多依赖价格昂贵的进口抗血清,实验结果的判断易受多种因素的影响,难以鉴别稀有的Rh变异型等。特别对于产前亲子鉴定和曾经接受过多次输血人员的血型鉴定与亲子鉴定等特殊的法医学问题,传统方法更是束手无策。近年来,随着Rh系统分子生物学研究的深入,RH基因分型技术应运而生,为解决以上难题提供了新的思路,成为研究热点。目前国内尚无应用基因分型技术检测Rh血型的相关报道,而RH基因在不同人种间的变异使国外的研究成果不能直接应用于中国人群。因此,本研究应用序列特异性引物PCR(PCR-SSP)技术对两个不同的中国群体进行RHCE基因型分布的调查,旨在探讨这一新兴技术的应用价值。材料和方法 1.样品来源 1.1 群体调查样品 200例中国北方汉族健康、无关个体血样由中国医科大学法医学院提供,200例中国南方黎族健康、无关个体血样由海南医学院提供。 1.2 亲子鉴定样品 5例亲子鉴定案件样品由中国医科大学法医学院提供。 2.实验方法 2.1 Rh血型血清学分型:应用单克隆抗-D、抗-C、抗-c、抗-E及
[Abstract]:Preface Rh blood group system is one of the most complex and polymorphic human blood group systems, which has important clinical significance. At the same time, as an excellent genetic marker, Rh blood group has been widely used in forensic practice since its discovery. At present, the Rh blood group typing method in China is a traditional serological technique, which has many disadvantages in practical application. For example, the experiment relies on the expensive imported antiserum, and the judgement of the experimental results is easily influenced by many factors, so it is difficult to identify the rare Rh variant and so on. Especially for the special forensic problems such as prenatal paternity testing and blood type identification and paternity testing of people who have received multiple blood transfusions, the traditional methods are even more helpless. In recent years, with the in-depth study of Rh molecular biology, RH genotyping technology emerged as the times require, which provides a new way to solve the above problems and becomes a research hotspot. At present, there are no reports on the application of genotyping technique in the detection of Rh blood group in China, but the variation of RH gene in different ethnic groups makes it impossible for foreign research results to be directly applied in Chinese population. Therefore, the RHCE genotypes of two different Chinese populations were investigated by sequence specific primer PCR-SSPP, in order to explore the application value of this new technique. Materials and methods 1. Sample source 1.1 Group Survey samples The unrelated individual blood samples were provided by the School of Forensic Medicine of China Medical University in 200 cases of Li nationality health in southern China, and the unrelated individual blood samples were provided by Hainan Medical College. 1.2 paternity test samples The samples of 5 cases of paternity test were provided by the School of Forensic Medicine of China Medical University. 2. Experimental method 2.1 serological typing of Rh blood groups: monoclonal anti--D-, anti--C-, anti--c-, anti--E and
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：D919.1

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