常染色体STR鉴定同胞的应用探讨

发布时间：2018-05-18 02:37

本文选题：常染色体 + 同胞鉴定　；参考：《中山大学学报(医学科学版)》2006年03期

【摘要】：目的探讨常染色体STR遗传标记鉴定两个体同胞关系的可行性。方法用PowerPlexTM16体系15个常染色体STR基因座检测50对全同胞、25对半同胞和50对无关个体,ITO法计算全同胞关系指数(FSI)、半同胞关系指数(HSI)和FSI∶HSI值,比较3组两个体间等位基因匹配情况,并进行组间差异的χ2检验。结果全同胞的FSI均大于1;19对半同胞的HSI大于1;无关个体FSI均小于1;49对无关个体HSI小于1,1对为1.297。46对全同胞的FSI:HSI值大于1,22对半同胞的FSI:HSI值小于1。全同胞组两个体间1对等位基因全相同、半相同、全不同的基因座数平均值分别为6.06个、7.52个、1.42个;半同胞组分别为2.96个、8.48个、3.56个;无关个体组分别为1.24个、7.22个、6.54个。χ2检验3组两个体间1对等位基因全相同和全不同的基因座数差异有显著意义,半相同的基因座数差异无显著意义。结论PowerPlexTM16体系在全同胞-无关个体的鉴定中效率较高,在鉴定半同胞-无关个体或全同胞-半同胞时效率降低。全同胞-半同胞关系鉴定时,FSI∶HSI值是一个较有效的指标:大于1倾向于为全同胞,小于1倾向于为半同胞。
[Abstract]:Objective to investigate the feasibility of autosomal STR markers in the identification of sibling relationship. Methods Fifteen autosomal STR loci of PowerPlexTM16 system were used to detect 50 pairs of whole sibling 25 pairs of half sibs and 50 pairs of unrelated individuals to calculate the whole sib relation index (FSI) and the FSI:HSI value. The allele matching between the three groups was compared and 蠂 2 test was performed. Results the FSI of the whole sibling was greater than that of the 1 / 19 / half sib, and the FSI of the unrelated individuals was less than 1 / 49 / 1 / 1 respectively. The FSI:HSI value of the whole sibling was 1.297.46. The FSI:HSI value of the whole sibling was greater than that of the 1 / 22 / half sib, and the FSI:HSI value of the two pairs was less than 1 / 1. The alleles of 1 pair of alleles in the whole sib group were all the same, half the same, and the average number of the alleles of the two alleles were 6.06, 7.52, 1.42, 2.96, 8.48, 3.56, respectively, in the half sib group. There were 1.24 alleles in unrelated individuals and 6.54 alleles in 7.22 individuals. There were significant differences in the number of alleles of 1 pair of alleles between the two groups, but there was no significant difference in the number of semi-identical loci between the three groups. Conclusion the efficiency of PowerPlexTM16 system is higher in the identification of whole sib-independent individuals, but lower in the identification of half-sib-independent individuals or whole sib-half sibs. The FSI: HSI value of FSI / half sibling relation is a more effective index: more than 1 tends to be whole sibling and less than 1 tends to be half sib.
【作者单位】：中山大学基础医学院法医物证学教研室中山大学基础医学院法医物证学教研室中山大学基础医学院法医物证学教研室中山大学基础医学院法医物证学教研室中山大学基础医学院法医物证学教研室中山大学基础医学院法医物证学教研室中山大学基础医学院法医物证学教研室
【基金】：广东省重点科技攻关项目(2002B31003)
【分类号】：D919

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