人体胸骨骨髓DNA降解与死亡时间的关系

发布时间：2018-05-29 21:49

本文选题：死后间隔时间 + 胸骨骨髓　；参考：《山西医科大学》2006年硕士论文

【摘要】：目的探讨死后人体胸骨骨髓DNA的降解与较长死后间隔时间(PMI)的关系。旨在为法医学上推断死亡时间提供科学参考依据。方法选取尸体胸骨15例，PMI确定，死亡环境、死亡原因已知。10％中性甲醛固定，经乙醇梯度脱水、二甲苯透明、浸蜡处理后，石蜡包埋，切片。应用普通HE染色、甲基绿派洛宁染色，显微镜下观察。利用计算机图像分析技术对死后不同时间(1h—7d)的胸骨骨髓DNA的甲基绿派洛宁染色情况进行分析，检测其含量参数，进行统计学分析，，寻找随着PMI的延长其含量参数的变化规律。结果尸体15例胸骨中，随PMI的延长，积分光密度(Integral Optical Density，IOD)、平均光密度(Average Optical Density，AOD)、平均灰度(Average Gray，AG)、分布密度(Distributing Density，DD)、目标面积(Aimed Area，AA)、光密度比率(Optical Density Ratio，ODR)和目标面积比率(Aimed Area Ratio，AAR)等参数呈现不同的变化趋势，其中，积分光密度、平均灰度和目标面积比与PMI之间存在明显的相关性。结论本实验应用甲基绿派洛宁染色法、普通HE染色法及计算机图像分析技术对人死后不同时间的胸骨骨髓DNA含量进行了定量研究。得出以下结论：1、人体胸骨骨髓DNA降解可以作为推断PMI的指标。2、测定机体死后胸骨骨髓DNA降解可以推断一定时间段PMI。
[Abstract]:Objective to investigate the relationship between the degradation of DNA in the bone marrow of human body after death and the longer interval of postmortem interval (PMI), and to provide a scientific reference for the inference of death time in forensic medicine.
Methods 15 cases of cadaver sternum were selected, PMI determined, the death environment, the cause of death known.10% neutral formaldehyde fixed, ethanol gradient dehydration, dimethylbenzene transparent, paraffin embedded, paraffin embedding, slice. Using ordinary HE dyeing, methyl green Luoning staining, under microscope observation. Computer image analysis technology for different time after death (1H - 7D) The staining of methyl green pies of DNA in the bone marrow of the sternum was analyzed and the content of the DNA was detected. The statistical analysis was carried out to find the change rule of the content parameters with the extension of PMI.
Results with the extension of PMI, the integral light density (Integral Optical Density, IOD), the average optical density (Average Optical Density, AOD), the average gray level (Average Gray, AG), the distribution density, the target area ratio, the ratio of the target area, and the target area ratio were the 15 cases of the body. D Area Ratio (AAR) and other parameters show different trends. There is a clear correlation between integral optical density, mean gray area and target area ratio and PMI.
Conclusion the content of DNA in the bone marrow of the sternum at different time after death was studied by the methylene green Luoning staining method, the common HE staining and the computer image analysis. The following conclusions were drawn as follows: 1, the DNA degradation of the bone marrow of the human body can be used as an indicator of PMI, and the determination of DNA degradation in the sternum bone marrow after the death of the body can be pushed forward. Break a certain period of PMI.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919

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