利多卡因在硬膜外麻醉致死犬体内分布的研究

发布时间：2018-06-12 18:36

本文选题：利多卡因 + 硬膜外麻醉致死　；参考：《山西医科大学》2005年硕士论文

【摘要】：目的: 1 建立犬利多卡因硬膜外麻醉致死动物模型,观察利多卡因硬膜外麻醉致死过程的生命体征和死后组织脏器的病理改变; 2 研究利多卡因在硬膜外麻醉致死犬体内分布,为利多卡因硬膜外麻醉致死案件的检材采取、检测、结果分析、死因判定及法医学鉴定提供科学依据。方法 1 动物模型犬9 只,随机分为实验组(6 只)和对照组(3 只),实验组犬5 分钟内硬膜外腔注射利多卡因5×15mg/kg,对照组犬注入等体积生理盐水。 2 生命体征记录方法BL-生物机能实验系统全程记录从开始给药到动物死亡的心电、血压和呼吸等生命体征的变化; 3 样品采集与处理心电、血压和呼吸全部消失时,迅速解剖动物,取大脑、侧脑室脑脊液、背侧脑脊液、不同节段脊髓(颈段脊髓、胸段脊髓、腰段脊髓、骶段脊髓)、心、肺、肝、脾、肾、胆汁、尿、心血、周围血、注射部位肌肉和注射部位20cm 以外肌肉等组织脏器和体液,即刻检测实验犬各组织和体液中利多卡因含量,对照组犬各脏器和体液用作空白检材; 4 病理观察取大脑、脊髓、心、肺、肝、脾、肾,用4%甲醛固定,石蜡包埋,切片,HE 染色,光镜观察; 5 检测利多卡因方法样品经酸、碱处理后,乙醚萃取,气相色谱和气相色谱-质谱联用检测,根据利多卡因的特征离子峰、保留时间定性与内标法、工作曲线法定量。结果 1 症状实验组犬在注药开始后5~8min 即出现瞳孔缩小、呼吸困难、烦躁、流涎、口吐粘稠状白沫、肌肉震颤、对外界刺激敏感等中毒症状,
[Abstract]:Objective: 1 to establish a canine animal model of lidocaine epidural anesthesia. To observe the vital signs of lidocaine during epidural anesthesia and the pathological changes of tissues and organs after death. 2 to study the distribution of lidocaine in dogs killed by epidural anesthesia. To provide scientific basis for the examination, detection, analysis of results, cause of death and forensic identification of lidocaine. Methods 1 Nine animal model dogs were used. Six rats were randomly divided into experimental group (n = 6) and control group (n = 3). Lidocaine (5 脳 15 mg / kg) was injected into the epidural cavity of the experimental group within 5 minutes, and the control group was injected with normal saline of the same volume. Physical signs recording method BL-Biological function Experimental system recorded ECG from the beginning of administration to the death of animals during the whole course. Changes of vital signs such as blood pressure and respiration. 3 sample collection and treatment of ECG. When blood pressure and respiration all disappeared, animals were dissected quickly, and cerebrospinal fluid (CSF) of the brain and lateral ventricle were taken. Dorsal cerebrospinal fluid, different segments of spinal cord (cervical spinal cord, thoracic spinal cord, lumbar spinal cord, sacral spinal cord, heart, lung, liver, spleen, kidney, bile, urine, heart blood, peripheral blood, The contents of lidocaine in all tissues and body fluids of dogs were detected immediately after injection, muscle and other tissues other than 20cm, and the contents of lidocaine in all tissues and body fluids of experimental dogs were detected immediately. (4) the brain, spinal cord, heart, lung, liver, spleen, kidney were fixed with 4% formaldehyde, embedded in paraffin, stained with HE in sections. Light microscope observation; (5) determination of lidocaine after acid and alkali treatment, ether extraction, gas chromatography and gas chromatography-mass spectrometry, according to the characteristic ion peak of lidocaine, Retention time qualitative and internal standard method, working curve measurement. Results (1) in the experimental group, pupillary constriction, dyspnea, salivation, mucous foam in mouth and muscle tremor appeared in 5~8min. Toxic symptoms such as sensitivity to external stimuli,
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：D919.1

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