十个新的Y染色体STR的特性及复合扩增研究

发布时间：2018-06-13 02:34

本文选题：法医学 + Y染色体　；参考：《四川大学》2004年博士论文

【摘要】：目的本课题旨在提高Y染色体的鉴别能力,为法医Y染色体分型提供新的遗传标记。对十个新的Y染色体STR基因座进行序列研究和单倍型分析,为等位基因的命名奠定基础,为法医学应用提供概率计算依据。选择其中四个基因座,依托毛细管电泳激光荧光检测平台,应用荧光标记复合扩增技术,建立一套新的Y染色体STR基因座复合扩增体系,并按照DNA分析方法技术工作组(The Technology Working Group on DNA Analysis Methods, TWGDAM)的指导方案进行法医学应用研究,以评估其在法医鉴定中的可用性。方法选择十个新Y-STR基因座,PCR扩增四川成都汉族103个无关男性DNA样本,PCR扩增产物使用聚丙烯酰胺凝胶电泳分离,银染法显色,以获得其群体遗传数据。选取其中四个Y-STR基因座,用自行设计的荧光标记引物复合扩增,毛细管电泳荧光自动检测系统对复合扩增产物进行检测和分型;用混合样本、不同的组织样本、不同介质上的DNA样本和常见动物的DNA作为模板,在不同的条件下对复合扩增系统进行测试和优化,用实际案例进行验证。结果在我们研究的103个成都汉族男性样本中,十个Y染色体STR基因座各观察到3～8个等位基因,等位
[Abstract]:Objective to improve the differentiation ability of Y chromosome and to provide a new genetic marker for forensic Y chromosome typing. The sequence study and haplotype analysis of ten new Y chromosome STR loci were carried out, which laid the foundation for allele naming and provided the basis of probability calculation for forensic application. Four of these loci were selected, and a new system of Y chromosome STR locus multiplex amplification was established based on capillary electrophoresis laser fluorescence detection platform and fluorescence labeling multiplex amplification technique. Forensic applied research was carried out under the guidance of the Technology working Group on DNA Analysis methods (TWGDAM) to assess its availability in forensic identification. Methods Ten new Y-STR loci were selected for PCR amplification of 103 unrelated male DNA samples from Sichuan Han nationality in Chengdu. Polyacrylamide gel electrophoresis was used to isolate the PCR products. Four Y-STR loci were selected for multiplex amplification with self-designed fluorescent labeled primers, and the multiplex amplification products were detected and typed by capillary electrophoresis fluorescence automatic detection system. DNA samples from different media and DNA from common animals were used as templates to test and optimize the multiplex amplification system under different conditions. Results among the 103 male samples of Chengdu Han nationality, 38 alleles and alleles were observed in 10 STR loci of Y chromosome.
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2004
【分类号】：D919

【引证文献】