单细胞检验技术用于混合上皮细胞的分离和检验

发布时间：2018-06-14 07:47

本文选题：法医物证学 + 单细胞检验技术　；参考：《中国法医学杂志》2011年01期

【摘要】：目的建立单细胞显微捕获联合低体积扩增技术,用于混合上皮细胞检材分离检验。方法取5名男性口腔上皮细胞拭子浸泡液30μL,分别滴加到5份含同一女性皮肤表皮细胞拭子上,制成5份混合上皮细胞样本为实验组,同时制备同样的5份样本为对照组。实验组样本采用显微捕获单个口腔上皮细胞,并使用低体积扩增技术进行扩增;对照组用M48纯化试剂盒提取DNA,Identifiler试剂盒复合扩增,扩增体系为10μL。所有产物均用ABI 3130遗传分析仪进行STR分型。结果 5份实验组样本均得到男性STR分型结果,5份对照组样本则均仅得到混合分型结果。将该方法应用于1例强奸杀人案例检验,取得了满意效果。结论单细胞显微捕获联合低体积扩增技术可用于混合上皮细胞样本的分离检验。
[Abstract]:Objective to establish a technique of single cell microcapture combined with low volume amplification for the separation and detection of mixed epithelial cell samples. Methods the oral epithelial cell swabs of 5 male patients were treated with 30 渭 L oral epithelial cell swabs. Five samples of mixed epithelial cells were prepared as experimental group and the same 5 samples were prepared as control group. In the experimental group, a single oral epithelial cell was captured microscopically and amplified by low volume amplification, while in the control group, the DNA identifiler was extracted by M48 purification kit, and the amplification system was 10 渭 L. All the products were classified by ABI 3130 genetic analyzer. Results the results of STR typing were obtained from 5 experimental group samples and only mixed typing results were obtained from 5 control group samples. The method has been applied to a case of rape homicide, and satisfactory results have been obtained. Conclusion single cell microcapture combined with low volume amplification can be used to separate and test mixed epithelial cell samples.
【作者单位】：中国人民公安大学;上海市公安局物证鉴定中心;公安部物证鉴定中心;南京市公安局浦口分局;
【基金】：国家“十一五”科技支撑计划项目(2006BAK07B01)
【分类号】：D919

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