失血性休克大鼠海马中HSP70的表达及死后稳定性研究

发布时间：2018-06-23 21:02

  本文选题：失血性休克 + 热休克蛋白　； 参考：《重庆医科大学》2008年硕士论文

【摘要】： 背景及目的:失血性休克死亡是交通事故、医疗责任事故以及刑事犯罪中一个常见的死因。目前对于失血性休克的研究,多集中于失血性休克的补液治疗及缺血-再灌注损伤的机制方面,而对失血性休克中出血至死亡所经历时间的推断,则报道甚少。至今对失血性休克发生后伤者存活时间的推断,仍没有一个公认的标准,也成为法医病理学上的一大难题。热休克蛋白70(heat shock protein 70,HSP70)是机体遭受缺血、缺氧、创伤、感染等各种应激性刺激后诱导HSP70基因的表达而产生的一种热休克蛋白,它可在多种细胞中表达,并呈现出一定的规律性。本实验主要研究大鼠失血性休克后海马中HSP70蛋白的表达和其死后稳定性,并探讨其法医学意义。 方法:1、选材:实验动物来源于重庆医科大学动物实验中心饲养的健康的清洁的SD大鼠105只,按照随机的原则将大鼠分为正常组、假手术组、失血性休克组、创伤对照组和死后稳定性组。失血性休克组与死后稳定性组采用改良Wigger’s失血性休克模型,创伤对照组参照Feeney’S自由落体模型装置制作大鼠脑挫裂伤模型;2、取材:在失血性休克/创伤后不同时间点取大鼠海马组织;3、对取材海马组织石蜡包埋切片进行HE染色光镜观察;4、采用免疫组织化学染色法进行HSP70染色;5、使用SPSS 10.0软件包对所得数据进行统计学分析。 结果:失血性休克1小时后,海马组织和血管壁即可见HSP70阳性表达,此后表达逐渐增强,至2小时呈强阳性表达(P0.01),部分锥体细胞核内也可见阳性表达。创伤对照组仅在血管壁和红细胞见HSP70阳性表达,海马组织HSP70表达呈弱阳性。死后稳定性组表达情况与休克2小时组相似(P0.05)。 结论:大鼠失血性休克后海马中HSP70蛋白的表达具有一定的规律性,呈现出逐渐升高-高峰期的变化,其表达水平在伤后2小时达到高峰,且在-20℃条件下,死后稳定性好。因此,HSP70蛋白可作为法医学失血性休克后个体存活时间推断的一个参考指标。
[Abstract]:Background and objective: hemorrhagic shock death is a common cause of death in traffic accidents, medical accidents and criminal offences. At present, the researches on hemorrhagic shock mainly focus on the rehydration therapy of hemorrhagic shock and the mechanism of ischemia-reperfusion injury, but there are few reports on the inference of the time between bleeding and death in hemorrhagic shock. So far, the estimation of the survival time of the injured after hemorrhagic shock has not been a recognized standard, and has become a major problem in forensic pathology. Heat shock protein 70 (heat shock protein 70) is a heat shock protein (HSP70) which is induced by various stress stimuli, such as ischemia, hypoxia, trauma, infection and so on. It can be expressed in many kinds of cells and has a certain regularity. In this study, the expression and postmortem stability of HSP70 protein in hippocampus of rats with hemorrhagic shock were studied and its forensic significance was discussed. Methods: 105 healthy and clean SD rats were collected from the Animal experiment Center of Chongqing Medical University. According to the random principle, the rats were divided into normal group, sham operation group and hemorrhagic shock group. Trauma control group and postmortem stability group. The hemorrhagic shock group and the postmortem stability group were treated with modified Wiggerers hemorrhagic shock model. The traumatic control group made rat brain contusion and laceration model according to FeeneyeyS free-fall model device. The materials were taken from hippocampus tissue of rats at different time points after hemorrhagic shock / trauma, and paraffin embedded sections of hippocampus tissue were obtained. HSP70 staining was performed by immunohistochemical staining and statistical analysis was carried out by SPSS 10.0 software package. Results: one hour after hemorrhagic shock, HSP70 positive expression was observed in hippocampal tissue and vascular wall, and then increased gradually, and was strongly expressed at 2 hours (P0.01). The positive expression was also found in some pyramidal nuclei. In trauma control group, HSP70 expression was only found in vascular wall and erythrocytes, and was weakly positive in hippocampal tissue. The expression of postmortem stability group was similar to that of shock group for 2 hours (P0.05). Conclusion: the expression of HSP70 protein in hippocampus of rats with hemorrhagic shock has a certain regularity, and the expression level reaches its peak at 2 hours after injury, and the postmortem stability is good at -20 鈩，

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