心脏电击伤的实验研究

发布时间：2018-06-29 04:32

本文选题：心脏 + 电损伤　；参考：《重庆医科大学》2005年博士论文

【摘要】：目的:电击伤(死)案件是法医实践中的常见案件,法医诊断电击伤主要是根据皮肤电流斑、金属化等特殊病理改变,但这些典型病理改变出现率低,使用范围有限。心脏是电生理活动最频繁的器官之一,电击特别是致死性电击常会出现一系列明显的病理及病理生理改变,能否利用这些改变诊断心脏电击伤,目前存在争议。为深入了解这些改变的法医学价值,本文从病理改变、基因表达方面对其进行了研究。方法:1.利用 220V 交流电(50Hz, 150-200 mA)电击 Wister大白鼠胸部(心脏体表投影位置)20s 复制动物模型。2.电击后在不同时间(30min、1h、2h、3h、6h、12h、1d、2d、3d、6d 及 12d)取材作 HE、Masson、HBFP 染色,观察其组织病理学改变;作超薄切片,利用 TEM 观察其超微组织病理学改变;以 FN 为一抗标记不同时间的心脏组织,以观察其分布情形;利用 TUNEL 原位杂交法观察心肌细胞核的改变。3. 利用 BioStar-40 基因芯片研究电击后 3h 心脏组织的基因表达图谱。4.观察 PEG-3,α-fibrinogen,Hyal2 和 APPILS 四个基因在不同时间点(1h、2h、3h、6h、8h、12h、1d、2d、3d、6d 及 12d)的变化规律。结果:光镜(LM)观察 30min-6h,心肌多灶性变性坏死,广泛心肌断裂、缺血,部分心肌细胞呈波纹状排列及收缩带坏死;心肌胞浆凝聚、均质变和嗜酸变,部分心肌横纹不清,胞核固缩、深染、
[Abstract]:Objective: electric injury (death) case is a common case in forensic practice. Forensic electrical injury is mainly based on special pathological changes of skin current spot and metallization, but these typical pathological changes are low and limited in use. The heart is one of the most frequent organs of electrophysiological activity, and electric shock, especially fatal electric shock, often appears one. A series of obvious pathological and pathophysiological changes and the possibility of using these changes to diagnose the electrical injury of the heart have been disputed. In order to understand the forensic value of these changes, this article has studied it from the aspect of pathological changes and gene expression.
Methods: 1. using 220V alternating current (50Hz, 150-200 mA) electric shock of Wister rat's chest (the location of the body surface of the heart) 20s replicating animal model.2. electric shock at different time (30min, 1H, 2h, 3h, 6h, 12h), observe its histopathological changes; make ultrathin slices, observe its ultrathin section, and observe its ultrastructure Histopathological changes; the distribution of cardiac tissue with FN as a marker of resistance to different times was observed. The changes in the nucleus of the myocardium were observed by TUNEL in situ hybridization..3. using BioStar-40 gene chip to study the gene expression profiles of 3H cardiac tissue after the shock of the electric shock,.4. observed the four bases of PEG-3, alpha -fibrinogen, Hyal2 and APPILS. Because of the change rule at different time points (1H, 2h, 3h, 6h, 8h, 12h, 1D, 2D, 3D, 6D and 12D).
Results: LM was used to observe 30min-6h, multifocal degeneration and necrosis of myocardium, extensive myocardial rupture, ischemia, partial myocardial cells in ripple arrangement and contraction zone necrosis, myocardial cytoplasm condensation, homogenization and eosinophilia, partial myocardial transverses, nucleus contraction and deep staining.
【学位授予单位】：重庆医科大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：D919

【共引文献】