大鼠电流损伤多器官c-Fos与Caspase-8蛋白的表达研究

发布时间：2018-07-03 05:36

本文选题：电流损伤 + c-fos　；参考：《四川大学》2004年硕士论文

【摘要】：研究背景: 法医学中,电流斑是判定是否存在电流损伤的主要依据。然而,一方面生前电流损伤可形成电流斑,死后遭受电流损伤也可形成电流斑,但生前与死后形成的电流斑在形态学上难以区别;另一方面法医学实践中也经常遇到没有明显电流斑的电击伤或电击死(如水中电击死),此类案件要诊断电流损伤或电击死极为困难。 c-fos是一种即刻早期表达基因(Immediately early genes, IEGs),很多研究表明机体受到刺激后,短时间内受刺激部位将出现c-fos蛋白的表达。因此,本研究探讨机体电流损伤后多器官c-fos蛋白的表达情况。研究目的: 1、检测电流损伤后大鼠多器官多部位c-fos的表达情况,探讨生前电击与死后电击的c-fos表达有何不同,能否用于生前电击与死后电击的鉴别; 2、探讨水中电击死亡是否有c-fos的表达,能否用于水中电击死亡的法医学鉴定。研究方法: 本实验包括以下三个方面: 1、生前直接电击:取雄性健康SD大鼠35只,随机分成7组,每组5只大鼠,分别为直接电击死、电击伤后1hr(hour)、2hr、4hr、8hr组,及实验对照组与正常对照组;实验组大鼠予以220V交流电电击:以电源零
[Abstract]:Background: in forensic medicine, current spot is the main basis for judging the existence of current damage. However, on the one hand, the current spot can be formed by the antemormal current injury and the current spot after death, but it is difficult to distinguish the morphologically between the antemortem and the postmortem current spot. On the other hand, in the practice of forensic medicine, there are often electric injuries or death without obvious current spots (such as electrocution in water), so it is very difficult to diagnose electric current injury or electrocution death in such cases. C-fos is an immediate early genes (IEGs), and many studies have shown that the body is stimulated. In a short period of time, the expression of c-fos protein will appear in the stimulated site. Therefore, this study was to investigate the expression of c-fos protein in multiple organs after electrical injury. Objective: 1. To detect the expression of c-fos in multiple organs and parts of rats after electric current injury, and to explore the difference of c-fos expression between antemortem shock and postmortem shock. 2. To investigate whether there is c-fos expression in electrocution death in water and whether it can be used in forensic identification of electric shock death in water. Research methods: this experiment includes the following three aspects: 1. Direct electric shock before death 35 healthy male SD rats were selected. The rats in each group were randomly divided into 7 groups, 5 rats in each group were killed by direct electric shock, the rats in the experimental group were given 220V AC electric shock with zero power source, the rats in the experimental group were given 220V AC electric shock: the rats in the experimental group were given 220V AC electric shock: the rats in the experimental group were given 220V AC electric shock with zero power source.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2004
【分类号】：D919

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