过敏性猝死豚鼠体内肥大细胞超微结构及P物质变化的法医病理学研究

发布时间：2018-07-08 07:53

本文选题：过敏性休克 + 豚鼠　；参考：《山西医科大学》2004年硕士论文

【摘要】：目的建立豚鼠过敏性休克动物模型，观察其死后病理变化，并检测血浆中P物质（Substance P, SP）的浓度，探讨P物质在过敏性休克中的作用，试图为过敏性休克的法医学诊断提供客观的诊断依据。方法 18只豚鼠，随机分为实验组和对照组（每组各9只）。实验组采用多人混合血清致敏豚鼠，建立过敏性休克死亡的动物模型。取豚鼠血浆及脏器组织（咽喉、肺、胃、结肠）。（1）放射免疫学方法测定血浆P物质含量；（2）石蜡切片，常规HE染色；（3）应用免疫组织化学SABC法进行P物质染色，并应用BI-2000图像分析系统进行图像分析，计算阳性指数（PI）；（4）透射电镜观察肥大细胞形态。结果（1）实验组血浆中P物质的含量明显高于对照组（实验组131.01±18.93pg/μl，对照组87.70±7.60pg/μl，P＜0.01）；（2）实验组HE染色在光镜下形态表现为急死的非特异性改变，对照组未见明显病理变化；（3）免疫组化染色，P物质在实验组的咽喉、肺、胃、结肠等组织中表达明显增加（咽喉组织实验组PI＝63.59±14.51，对照组PI=33.32±8.04 ，P＜0.01；肺组织实验组PI=55.98±14.8，对照组PI＝20.51±6.76，P＜0.01；胃组织实验组PI＝18.97±6.69，对照组PI=13.32±2.48，，＜0.01；结肠组织实验组PI＝89.22±13.87，对照组PI=53.20±15.32，P＜0.01）；（4）过敏性休克豚鼠肥大细胞超微结构呈脱颗粒状，对照组豚鼠肥大细胞的胞浆内颗粒丰富，未见脱颗粒现象。结论过敏性休克时，豚鼠血浆中P物质含量增高，咽喉、肺、胃、结肠等组织中P物质表达增加，肥大细胞呈脱颗粒的表现。因此，应用放射免疫法测定血浆中P物质的含量和免疫组化法对咽喉、肺、胃、结肠中P物质进行染色，并结合肥大细胞脱颗粒的超微结构，可为过敏性休克的法医学诊断提供客观的血清学和形态学依据。
[Abstract]:Objective to establish an animal model of anaphylactic shock in guinea pigs, observe the pathological changes after death, detect the concentration of substance P (SP) in plasma, and explore the role of substance P in anaphylactic shock. This paper attempts to provide objective diagnostic basis for forensic diagnosis of anaphylactic shock. Methods 18 guinea pigs were randomly divided into experimental group and control group (9 in each group). The experimental group was sensitized by mixed human serum to establish the animal model of anaphylactic shock death. Plasma and organ tissues (throat, lung, stomach, colon). (_ 1) were taken from guinea pigs to determine the content of substance P by radioimmunoassay; (2) paraffin sections were stained with routine HE staining; (3) substance P staining was performed with immunohistochemical SABC method. BI-2000 image analysis system was used to analyze the morphology of mast cells. The positive index (Pi); (4) was calculated by transmission electron microscope. Results (1) the content of substance P in plasma in the experimental group was significantly higher than that in the control group (131.01 卤18.93pg/ 渭 l in the experimental group and 87.70 卤7.60pg/ 渭 l-1 in the control group, P < 0. 01); (2). (3) the expression of substance P in laryngopharynx, lung, stomach and colon of the experimental group was significantly higher than that of the control group (63.59 卤14.51, 33.32 卤8.04, P < 0.01, 55.98 卤14.8, 20.51 卤6.76, P < 0.01, respectively). The ultrastructure of mast cells in gastric tissue test group (18.97 卤6.69), control group (13.32 卤2.48, < 0.01), colon tissue experiment group (89.22 卤13.87) and control group (53.20 卤15.32); (_ 4) showed degranulation. No degranulation was observed. Conclusion during anaphylactic shock, the content of substance P in plasma and the expression of substance P in larynx, lung, stomach and colon were increased, and mast cells were degranulated. Therefore, the content of substance P in plasma was determined by radioimmunoassay and the substance P in throat, lung, stomach and colon were stained by immunohistochemistry, and combined with the ultrastructure of mast cell degranulation. It can provide objective serological and morphological basis for forensic diagnosis of anaphylactic shock.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2004
【分类号】：D919.1

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