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毒鼠强中毒大鼠脑GABA及GABA_AR-α1表达的实验研究

发布时间:2018-08-16 17:52
【摘要】:研究背景及目的:毒鼠强是一种剧毒鼠药,对人、畜危害极大,临床诊治和法医学鉴定经常遇到毒鼠强中毒的案例,但是对毒鼠强中毒的机制仍不明确。GABA是中枢神经系统主要的抑制性神经递质;GABA_AR是GABA的受体之一,在脑内分布最普遍,α1亚单位与其功能密切相关。GABA及GABA_AR在癫痫、颅脑损伤、神经毒剂中毒等疾病、损伤和中毒模型中的表达和/或结合都有改变,但在毒鼠强中毒中的表达情况尚未见报道。本研究对毒鼠强中毒大鼠脑内GABA及GABA_AR-α1的表达情况和变化规律进行研究,以探讨GABA及GABA_AR与毒鼠强中毒的关系,为研究毒鼠强中毒机制提供实验依据,并为法医学上毒鼠强中毒鉴定提供可能的新途径。 方法:健康Sprague-Dawley大鼠(S-D大鼠)60只,随机分为正常对照组、空白对照组、急性中毒组和慢性中毒组,其中慢性中毒组按中毒后处死时间不同分成30min,3h,6h,12h,24h,3d,5d,7d,10d组,每组5只。急性中毒组每只以1.0mg/kg的剂量用毒鼠强悬浊液灌胃,慢性中毒组每只以0.1mg/kg的剂量用毒鼠强溶液灌胃制作中毒模型。应用免疫组化技术和图像分析仪对GABA及GABA_AR-α1的表达情况和变化规律进行研究。 结果:①正常大鼠脑组织内GABA及GABA_AN-α1表达较为广泛,维持在中等水平;②急性中毒组脑内GABA及GABA_AR-α1表达均下降;
[Abstract]:Background and objective: tetramine is a highly toxic rodenticide, which is harmful to human and animal. The cases of tetramine poisoning are often encountered in clinical diagnosis, treatment and forensic medicine. However, the mechanism of tetramine poisoning is still unclear. GABA is one of the main inhibitory neurotransmitters in the central nervous system. GABAAR is one of the receptors of GABA, and the distribution of 伪 1 subunit is most common in the brain. The 伪 1 subunit is closely related to its function in epilepsy and brain injury. The expression and / or combination of nerve agent poisoning and other diseases, injury and poisoning models have been changed, but the expression in tetramine poisoning has not been reported. In this study, we studied the expression and changes of GABA and GABA AR- 伪 1 in the brain of rats with tetramine poisoning, in order to explore the relationship between GABA and GABA_AR and tetramine poisoning, and to provide experimental basis for studying the mechanism of tetramine poisoning. It also provides a new way for the identification of tetramine poisoning in forensic medicine. Methods: sixty healthy Sprague-Dawley rats (S-D rats) were randomly divided into normal control group, blank control group, acute poisoning group and chronic poisoning group. According to the time of death, chronic poisoning group was divided into three groups: 30min, 3h, 12h, 24h, 3h, 3d, 5d, 7d, 10d group. The acute poisoning group was given the dose of 1.0mg/kg and the chronic poisoning group was fed with tetramine suspension. The chronic poisoning group was given the dose of 0.1mg/kg to make the poisoning model. Immunohistochemical technique and image analyzer were used to study the expression and changes of GABA and GABAAR- 伪 1. Results the expression of GABA and GABAAR- 伪 1 in brain tissue of normal rats was more extensive, and the expression of GABA and GABAAR- 伪 1 in the brain of acute poisoning group was decreased.
【学位授予单位】:四川大学
【学位级别】:硕士
【学位授予年份】:2005
【分类号】:D919

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