太原汉族群体DYS390、DYS391和DYS393基因座多态性及法医学应用研究
发布时间：2018-08-19 13:47
【摘要】： 目的 (1) 建立DYS390、DYS391和DYS393基因座的复合扩增方法。(2) 调查太原地区汉族男性群体Y染色体DYS390、DYS391和DYS393基因座等位基因和单倍型频率分布以及群体差异情况，为群体遗传学、法医学个体识别及亲子签定提供计算数据。 方法 (1)样本采集：随机抽取163名太原地区汉族无亲缘关系的男性个体的静脉血，EDTA抗凝，进行DYS390、DYS391和DYS393基因座等位基因及单倍型频率分布调查；采集同一尸体血液、肌肉、肝脏、肾组织进行同一性检测；采集20例两代家系血进行突变观察；取20例女性个体血进行男性特异性检验。(2)DNA提取、PCR和测序：改良的TKM1法和Chelex-100法提取DNA，复合扩增Y染色体DYS390、DYS391 山两医丰}大学2003年硕卜学位论文 和。YS393二基因座，PAG电泳分型，银染显色。对侮一基因 座最高频率等位基因的进行测序分析。(3)数据处理:等位 基因及单倍型的频率采用直接计算法，与其他人群比较采用 FISher’S精确检验统计。 结果(1)3个基因座复合扩增显示:本实验设_、丫的条件卜， 3个华因座扩增均衡，且PAGE分型清晰，互不十扰，效果理 想C(2)太原地区汉族男性oys39o、DYs391和DYS393基 因座分别观察到5、6和5个等位基因，基因多样性分别为 0.70584、0.4476和0.56282。nYS390基因座共观察到6个等 位去誓区1，分别为DYS39O*19、DYS390*22、DYS39O*23、 Dy’5390*24、DYS390*25和DYS39O*26;DYS393基因座共 观察到5个等位基Ik1，分别为DYS393*11、DYS393*12 D丫5393*13、DYS393*14和DYS393*15;DYS391基I大l座共 观察到5个等位基因，分别为DYS391*东0YS391*9、 D、’5391‘10、DYS391*11和DYS391*12。对这3个基l天1座构 成的单倍型进行观察，，163例共检测到37种单倍型，单倍型 多样性为0.9485。FISher’S精确检验统计一分析一表明，DYS390、 山西医科大学2003年硕十学位论文 DY，391等位基因频率分布与国内不同地区差异不明显，与自 种人有明显差异。DYS393在国内与沈阳地区差异不显著，与 成都、台湾地区差异显著，与白种人差异显著。(3)同一尸 体血‘液、肌肉、肝脏和肾组织检测结果分型一致。(4)20例 两代家系观察，未见突变。(5)20例女性样本DNA无扩增 产物。 结论(1)Y染色体DYS39O、DYS391和DYS393基因座多 态性较高，本实验所得太原地区汉族男性DYS390、DYS391 和DYS393基因座等位基因和基因多样性数据可应用于法医 学鉴定和遗传学分析。 (2)本文所建立的DYS39O、DYS391和DYS393基区} 座的复合扩增体系具有简便、快捷、省材的特点，且分型准 确清晰，尤其适用于法医学检案。
[Abstract]:Objective (1) To establish a multiplex amplification method for DYS390, DYS39 1 and DYS393 loci. (2) To investigate the allele and haplotype frequencies of Y chromosome DYS390, DYS39 1 and DYS393 loci in Taiyuan Han male population, and to provide computational data for population genetics, forensic individual identification and parent-child signature.
Methods (1) Sample collection: 163 unrelated male individuals in Taiyuan were randomly selected for EDTA anticoagulation, DYS390, DYS39 1 and DYS393 loci allele and haplotype frequency distribution were investigated, blood, muscle, liver and kidney tissues of the same cadaver were collected for identity testing, and blood transfusion of 20 two-generation families were collected. DNA extraction, PCR and sequencing: DNA was extracted by modified TKM1 and Chelex-100 methods, and Y chromosome DYS390 and DYS391 were amplified.
Shan two, Feng Feng} University, 2003
And.YS393 two loci, PAG electrophoresis typing, silver staining.
The highest frequency alleles were sequenced and analyzed. (3) data processing: alleles.
The frequencies of genes and haplotypes were directly calculated and compared with other populations.
FISher 'S exact test statistics.
Results (1) 3 loci multiplex amplification showed that:
The 3 Huayin blocks were amplified and balanced, and the PAGE typing was clear, and there were no ten disturbances.
Think C (2) oys39o, DYs391 and DYS393 bases of Han nationality in Taiyuan area.
5,6 and 5 alleles were observed in the locus respectively.
6 0.70584,0.4476 and 0.56282.nYS390 loci were observed.
Take the oath area 1, DYS39O*19, DYS390*22, DYS39O*23, respectively.
Dy '5390*24, DYS390*25 and DYS39O*26; DYS393 loci
5 alleles Ik1, DYS393*11 and DYS393*12, were observed.
D ya 5393*13, DYS393*14 and DYS393*15; DYS391 based I large L block.
5 alleles were observed, DYS391* East 0YS391*9, respectively.
D, '5391', 10, DYS391*11 and DYS391*12. on these 3 bases l days 1 buildings.
Haplotypes were observed, and 37 haplotypes were detected in 163 cases.
Diversity is 0.9485.FISher 'S exact test statistics one analysis shows that DYS390,
Shanxi Medical University master degree thesis 2003 ten
The frequency distribution of DY and 391 alleles was not significantly different from that in different regions of China.
There is a significant difference between.DYS393 and Shenyang.
The difference between Chengdu and Taiwan is significant, and is significantly different from that of Caucasians. (3) the same corpse.
The results were identical in body fluid, muscle, liver and kidney. (4) 20 cases.
No mutation was observed in the two generation family. (5) there was no DNA amplification in 20 female samples.
Product.
Conclusion (1) the DYS39O, DYS391 and DYS393 loci of Y chromosome are numerous.
The male DYS390 of Han nationality in Taiyuan area was DYS391.
The allele and gene diversity data of DYS393 loci can be applied to forensic science.
Identification and genetic analysis.
(2) the DYS39O, DYS391 and DYS393 base areas established in this paper}
The multiplex amplification system is simple, fast and economical.
It is clear and clear, especially for forensic examination.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2003
【分类号】：D919
本文编号：2191836
[Abstract]:Objective (1) To establish a multiplex amplification method for DYS390, DYS39 1 and DYS393 loci. (2) To investigate the allele and haplotype frequencies of Y chromosome DYS390, DYS39 1 and DYS393 loci in Taiyuan Han male population, and to provide computational data for population genetics, forensic individual identification and parent-child signature.
Methods (1) Sample collection: 163 unrelated male individuals in Taiyuan were randomly selected for EDTA anticoagulation, DYS390, DYS39 1 and DYS393 loci allele and haplotype frequency distribution were investigated, blood, muscle, liver and kidney tissues of the same cadaver were collected for identity testing, and blood transfusion of 20 two-generation families were collected. DNA extraction, PCR and sequencing: DNA was extracted by modified TKM1 and Chelex-100 methods, and Y chromosome DYS390 and DYS391 were amplified.
Shan two, Feng Feng} University, 2003
And.YS393 two loci, PAG electrophoresis typing, silver staining.
The highest frequency alleles were sequenced and analyzed. (3) data processing: alleles.
The frequencies of genes and haplotypes were directly calculated and compared with other populations.
FISher 'S exact test statistics.
Results (1) 3 loci multiplex amplification showed that:
The 3 Huayin blocks were amplified and balanced, and the PAGE typing was clear, and there were no ten disturbances.
Think C (2) oys39o, DYs391 and DYS393 bases of Han nationality in Taiyuan area.
5,6 and 5 alleles were observed in the locus respectively.
6 0.70584,0.4476 and 0.56282.nYS390 loci were observed.
Take the oath area 1, DYS39O*19, DYS390*22, DYS39O*23, respectively.
Dy '5390*24, DYS390*25 and DYS39O*26; DYS393 loci
5 alleles Ik1, DYS393*11 and DYS393*12, were observed.
D ya 5393*13, DYS393*14 and DYS393*15; DYS391 based I large L block.
5 alleles were observed, DYS391* East 0YS391*9, respectively.
D, '5391', 10, DYS391*11 and DYS391*12. on these 3 bases l days 1 buildings.
Haplotypes were observed, and 37 haplotypes were detected in 163 cases.
Diversity is 0.9485.FISher 'S exact test statistics one analysis shows that DYS390,
Shanxi Medical University master degree thesis 2003 ten
The frequency distribution of DY and 391 alleles was not significantly different from that in different regions of China.
There is a significant difference between.DYS393 and Shenyang.
The difference between Chengdu and Taiwan is significant, and is significantly different from that of Caucasians. (3) the same corpse.
The results were identical in body fluid, muscle, liver and kidney. (4) 20 cases.
No mutation was observed in the two generation family. (5) there was no DNA amplification in 20 female samples.
Product.
Conclusion (1) the DYS39O, DYS391 and DYS393 loci of Y chromosome are numerous.
The male DYS390 of Han nationality in Taiyuan area was DYS391.
The allele and gene diversity data of DYS393 loci can be applied to forensic science.
Identification and genetic analysis.
(2) the DYS39O, DYS391 and DYS393 base areas established in this paper}
The multiplex amplification system is simple, fast and economical.
It is clear and clear, especially for forensic examination.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2003
【分类号】：D919
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