太原汉族群体DYS390、DYS391和DYS393基因座多态性及法医学应用研究
[Abstract]:Objective (1) To establish a multiplex amplification method for DYS390, DYS39 1 and DYS393 loci. (2) To investigate the allele and haplotype frequencies of Y chromosome DYS390, DYS39 1 and DYS393 loci in Taiyuan Han male population, and to provide computational data for population genetics, forensic individual identification and parent-child signature.
Methods (1) Sample collection: 163 unrelated male individuals in Taiyuan were randomly selected for EDTA anticoagulation, DYS390, DYS39 1 and DYS393 loci allele and haplotype frequency distribution were investigated, blood, muscle, liver and kidney tissues of the same cadaver were collected for identity testing, and blood transfusion of 20 two-generation families were collected. DNA extraction, PCR and sequencing: DNA was extracted by modified TKM1 and Chelex-100 methods, and Y chromosome DYS390 and DYS391 were amplified.
Shan two, Feng Feng} University, 2003
And.YS393 two loci, PAG electrophoresis typing, silver staining.
The highest frequency alleles were sequenced and analyzed. (3) data processing: alleles.
The frequencies of genes and haplotypes were directly calculated and compared with other populations.
FISher 'S exact test statistics.
Results (1) 3 loci multiplex amplification showed that:
The 3 Huayin blocks were amplified and balanced, and the PAGE typing was clear, and there were no ten disturbances.
Think C (2) oys39o, DYs391 and DYS393 bases of Han nationality in Taiyuan area.
5,6 and 5 alleles were observed in the locus respectively.
6 0.70584,0.4476 and 0.56282.nYS390 loci were observed.
Take the oath area 1, DYS39O*19, DYS390*22, DYS39O*23, respectively.
Dy '5390*24, DYS390*25 and DYS39O*26; DYS393 loci
5 alleles Ik1, DYS393*11 and DYS393*12, were observed.
D ya 5393*13, DYS393*14 and DYS393*15; DYS391 based I large L block.
5 alleles were observed, DYS391* East 0YS391*9, respectively.
D, '5391', 10, DYS391*11 and DYS391*12. on these 3 bases l days 1 buildings.
Haplotypes were observed, and 37 haplotypes were detected in 163 cases.
Diversity is 0.9485.FISher 'S exact test statistics one analysis shows that DYS390,
Shanxi Medical University master degree thesis 2003 ten
The frequency distribution of DY and 391 alleles was not significantly different from that in different regions of China.
There is a significant difference between.DYS393 and Shenyang.
The difference between Chengdu and Taiwan is significant, and is significantly different from that of Caucasians. (3) the same corpse.
The results were identical in body fluid, muscle, liver and kidney. (4) 20 cases.
No mutation was observed in the two generation family. (5) there was no DNA amplification in 20 female samples.
Product.
Conclusion (1) the DYS39O, DYS391 and DYS393 loci of Y chromosome are numerous.
The male DYS390 of Han nationality in Taiyuan area was DYS391.
The allele and gene diversity data of DYS393 loci can be applied to forensic science.
Identification and genetic analysis.
(2) the DYS39O, DYS391 and DYS393 base areas established in this paper}
The multiplex amplification system is simple, fast and economical.
It is clear and clear, especially for forensic examination.
【学位授予单位】:山西医科大学
【学位级别】:硕士
【学位授予年份】:2003
【分类号】:D919
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