脑红蛋白在大鼠全身组织的分布及大鼠外伤性脑损伤后脑红蛋白表达的实验研究

发布时间：2018-10-05 06:37

【摘要】：第一部分免疫组织化学方法检测脑红蛋白在大鼠全身组织的分布研究背景及目的:脑红蛋白(Neuroglobin, Ngb)是珠蛋白超家族中新近发现的一种珠蛋白,主要存在于脊椎动物神经系统,国内外文献未见用免疫组织化学方法检测Ngb蛋白在大鼠脑以外的全身其它各器官组织分布的报道。本研究应用免疫组织化学SP法研究Ngb在成年SD大鼠全身组织的分布和定位,为进一步探讨Ngb的生理功能提供详细的形态学资料。方法:雄性健康成年SD大鼠5只,经麻醉、灌注处死后,取全身各器官组织,经常规固定、包埋、制片,应用SP法进行免疫组织化学反应,观察Ngb的分布和定位情况。结果:(1)在所观察的大脑皮质、海马、丘脑、下丘脑、脑干、小脑和脊髓神经细胞都出现Ngb蛋白免疫阳性反应,不同区域表达强度不同:(2)在外周神经系统的神经节、有髓神经纤维和视网膜也观察到Ngb免疫阳性反应;(3)垂体、肾上腺、睾丸也可见到Ngb-IR阳性细胞。(4)在心肌、肺脏、肝脏、皮肤、骨骼肌、膀胱、脾脏、胰腺等其他组织器官Ngb蛋白免疫反应阴性。结论:本研究发现SD大鼠神经系统和功能活跃的内分泌器官有较多Ngb蛋白的分布,根据脑红蛋白可逆结合氧的特性,本研究结果提示Ngb可能对维持神经系统和一些内分泌器官的正常功能发挥重要作用,本研究为进一步探讨Ngb的形态和功能的关系提供了重要的实验资料。
[Abstract]:The first part: Immunohistochemical method to detect the distribution of globin in the whole body of rats background and objective: (Neuroglobin, Ngb) is a bead egg A newly discovered globin in the white superfamily, It mainly exists in the vertebrate nervous system. There is no report on the distribution of Ngb protein in all organs and tissues of the whole body except the brain of rats by immunohistochemical method. The distribution and localization of Ngb in adult SD rats were studied by immunohistochemical SP method in order to provide detailed morphological data for further study of the physiological function of Ngb. Methods: five male healthy adult SD rats were anesthetized and perfused to death. The organs and tissues of the whole body were taken and fixed, embedded and sliced. The distribution and localization of Ngb were observed by SP method. Results: (1) Ngb protein immunoreactivity was observed in the neurons of cerebral cortex, hippocampus, thalamus, hypothalamus, brainstem, cerebellum and spinal cord. Ngb immunoreactivity was also observed in myelinated nerve fibers and retina; (3) Ngb-IR positive cells were also found in pituitary, adrenal gland and testis. (4) Ngb-IR positive cells were found in myocardium, lung, liver, skin, skeletal muscle, bladder, spleen, The immunoreactivity of Ngb protein in other tissues such as pancreas was negative. Conclusion: in this study, we found that there were more Ngb proteins in nervous system and endocrine organs of SD rats. The results suggest that Ngb may play an important role in maintaining the normal function of nervous system and some endocrine organs. This study provides important experimental data for further study on the relationship between morphology and function of Ngb.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2004
【分类号】：D919

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