脑震荡鼠认知行为障碍及脑乙酰胆碱转移酶（ChAT）变化的研究
发布时间：2018-11-12 20:53
【摘要】：目的：应用脑震荡(Cerebral Concussion，CC)大鼠模型，观察不同损伤程度脑震荡后不同时间大鼠认知障碍变化及乙酰胆碱转移酶变化规律，探讨脑震荡鼠认知、记忆障碍的特点及与乙酰胆碱转移酶变化的关系，了解脑震荡鼠认知记忆行为障碍的神经生物学基础，为脑震荡后综合征的临床诊断、治疗提供理论依据。方法：应用自制金属单摆式闭合性脑损伤机械打击装置复制脑震荡大鼠模型；70只SD大鼠随机分为8组：大鼠单纯性脑震荡组(Pure Cerebral Concussion，PCC)伤后1、2、4、8、16、24天组共六个损伤组(n=10)、复杂性脑震荡组(Complicated Cerebral Concussion，CCC)和正常对照组(n=10)。应用平衡木(Beam Balance test，BBT)及噪音横杆跑动试验(Beam Walking test，BWT)，分别检测和伤后1～5天的平衡时间(Balance Latency，BL)以及步入黑箱的潜伏期(Step Through Latency，STL)的改变。应用免疫印记法(Western Blot)分别检测大鼠PCC伤后1、2、4、8、16、24天中隔区、基底节、前额叶和中脑的乙酰胆碱转移酶(Cholin Acetyltransferase，CHAT)的变化。结果：行为学结果显示PCC组平衡时间与对照组比较无差异；但在BWT中，仅在1、2天步入潜伏期显著延长。CCC组平衡时间与对照组比较，在伤后第1～3天差异具有显著性(p＜0.05)；在BWT中，，第1～5天步入潜伏期均显著延长，逐渐从48.12±18.24秒恢复至27.10±28.36秒。免疫印迹结果显示与对照组相比较，基底节ChAT伤后第2天开始上升，差异具有显著性(p＜0.05)；伤后第24天表达有所下降但仍未恢复至正常。中隔区ChAT伤后第1天开始上升，但差异不具有显著性(p＞0.05)；直至伤后第4～24天差异均具有显著性(p＜0.05)。前额叶ChAT伤后第1天开始下降，但差异不具有显著性(p＞0.05)；直至伤后第8～24天差异均具有显著性(p＜0.05)。中脑ChAT伤后第2天开始上升，差异具有显著性(p＜0.05)；直至伤后第24天差异均具有显著性(p＜0.05)。结论：1、脑震荡后大鼠在BBT和BWT实验中出现程度不同的近期学习记忆功能障碍，但PCC组在伤后损伤程度较轻、恢复快；CCC组在伤后损伤程度较重、恢复较慢。2、脑震荡大鼠中隔区、基底节、前额叶和中脑的ChAT活性表达有明显变化；3、脑震荡大鼠认知障碍与相关脑区ChAT活性表达的变化有关联。
[Abstract]:Objective: to observe the changes of cognitive impairment and acetylcholine transferase (acetylcholine transferase) in rats with cerebral concussion (Cerebral Concussion,CC) at different time and at different time after concussion, and to explore the cognition of concussion rats. The characteristics of memory disorder and its relationship with the changes of acetylcholine transferase (acetylcholine transferase), the neurobiological basis of cognitive memory disorder in concussion rats were understood, which provided theoretical basis for the clinical diagnosis and treatment of postconcussion syndrome. Methods: the rat model of cerebral concussion was established by using the mechanical striking device of metal monopodic closed brain injury. 70 SD rats were randomly divided into 8 groups: the rats with simple concussion group (Pure Cerebral Concussion,PCC) were divided into six groups (n = 10), complex concussion group (Complicated Cerebral Concussion,CCC) and normal control group (n = 10). Balance beam (Beam Balance test,BBT) and noise bar running test (Beam Walking test,BWT) were used to detect the changes of balance time (Balance Latency,BL) and incubation period (Step Through Latency,STL) of 1 day after injury. The changes of acetylcholine transferase (Cholin Acetyltransferase,CHAT) in the septum, basal ganglia, prefrontal lobe and midbrain of rats were detected by (Western Blot) on the 1st and 2nd day after PCC injury. Results: the behavioral results showed that there was no difference in balance time between PCC group and control group. However, in BWT, the incubation period was significantly prolonged only on 1 day and 2 days after injury. The balance time of CCC group was significantly different from that of control group on the 1st day after injury (p < 0. 05). In BWT, the incubation period was significantly prolonged on the 1st day, and gradually recovered from 48.12 卤18.24 seconds to 27.10 卤28.36 seconds. The results of immunoblotting showed that the expression of ChAT in basal ganglia began to rise on the second day after injury compared with the control group (p < 0. 05), and the expression of ChAT decreased on the 24th day after injury, but still did not return to normal. The ChAT in the septal area began to rise on the 1st day after injury, but the difference was not significant (p > 0. 05), but there was a significant difference up to the 4th day after injury (p < 0. 05). The ChAT of prefrontal lobe began to decrease on the 1st day after injury, but the difference was not significant (p > 0. 05), but the difference was significant up to the 8th day after injury (p < 0. 05). On the second day after ChAT injury, the difference was significant (p < 0. 05), and the difference was significant up to the 24th day after injury (p < 0. 05). Conclusion: 1. In the BBT and BWT experiments, the rats with cerebral concussion had different degrees of learning and memory impairment, but in the PCC group, the degree of injury was mild and the recovery was faster. In CCC group, the degree of injury was severe and the recovery was slower. 2. The expression of ChAT activity in septum, basal ganglia, prefrontal lobe and midbrain of rats with cerebral concussion showed significant changes. 3. Cognitive impairment was associated with the change of ChAT activity in related brain regions in concussion rats.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919
[Abstract]:Objective: to observe the changes of cognitive impairment and acetylcholine transferase (acetylcholine transferase) in rats with cerebral concussion (Cerebral Concussion,CC) at different time and at different time after concussion, and to explore the cognition of concussion rats. The characteristics of memory disorder and its relationship with the changes of acetylcholine transferase (acetylcholine transferase), the neurobiological basis of cognitive memory disorder in concussion rats were understood, which provided theoretical basis for the clinical diagnosis and treatment of postconcussion syndrome. Methods: the rat model of cerebral concussion was established by using the mechanical striking device of metal monopodic closed brain injury. 70 SD rats were randomly divided into 8 groups: the rats with simple concussion group (Pure Cerebral Concussion,PCC) were divided into six groups (n = 10), complex concussion group (Complicated Cerebral Concussion,CCC) and normal control group (n = 10). Balance beam (Beam Balance test,BBT) and noise bar running test (Beam Walking test,BWT) were used to detect the changes of balance time (Balance Latency,BL) and incubation period (Step Through Latency,STL) of 1 day after injury. The changes of acetylcholine transferase (Cholin Acetyltransferase,CHAT) in the septum, basal ganglia, prefrontal lobe and midbrain of rats were detected by (Western Blot) on the 1st and 2nd day after PCC injury. Results: the behavioral results showed that there was no difference in balance time between PCC group and control group. However, in BWT, the incubation period was significantly prolonged only on 1 day and 2 days after injury. The balance time of CCC group was significantly different from that of control group on the 1st day after injury (p < 0. 05). In BWT, the incubation period was significantly prolonged on the 1st day, and gradually recovered from 48.12 卤18.24 seconds to 27.10 卤28.36 seconds. The results of immunoblotting showed that the expression of ChAT in basal ganglia began to rise on the second day after injury compared with the control group (p < 0. 05), and the expression of ChAT decreased on the 24th day after injury, but still did not return to normal. The ChAT in the septal area began to rise on the 1st day after injury, but the difference was not significant (p > 0. 05), but there was a significant difference up to the 4th day after injury (p < 0. 05). The ChAT of prefrontal lobe began to decrease on the 1st day after injury, but the difference was not significant (p > 0. 05), but the difference was significant up to the 8th day after injury (p < 0. 05). On the second day after ChAT injury, the difference was significant (p < 0. 05), and the difference was significant up to the 24th day after injury (p < 0. 05). Conclusion: 1. In the BBT and BWT experiments, the rats with cerebral concussion had different degrees of learning and memory impairment, but in the PCC group, the degree of injury was mild and the recovery was faster. In CCC group, the degree of injury was severe and the recovery was slower. 2. The expression of ChAT activity in septum, basal ganglia, prefrontal lobe and midbrain of rats with cerebral concussion showed significant changes. 3. Cognitive impairment was associated with the change of ChAT activity in related brain regions in concussion rats.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919
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