HPLC法测定甲基苯丙胺与苯丙胺

发布时间：2019-01-06 09:46

【摘要】：目的建立甲基苯丙胺(methamphetamine,MA)和苯丙胺(amphetamine,AMP)的反相高效液相色谱测定方法。方法采用C18柱,以甲醇-磷酸盐缓冲液为流动相,流速1.0mL/min,检测波长为215nm,同时收集190~360nm的紫外光谱图,并以此与保留时间作为定性依据。在定性的基础上,建立定量检测方法,并对方法进行评价研究。结果所建方法能良好分离MA和AMP,结合判断依据能准确定性;MA在1.4~270μg/mL浓度范围内线性关系良好,R2=1,日内标准偏差(RSD)与日间RSD均2.4%,检出限为0.73μg/mL,平均加样回收率为102.5%;AMP在0.9~580μg/mL浓度范围内线性关系良好,R2=0.9999,日内RSD与日间RSD均2.3%,检出限为0.52μg/mL,平均加样回收率为101.7%。结论此方法简便、快速、准确,适用于MA与AMP的检测。
[Abstract]:Objective to establish a reversed-phase HPLC method for the determination of methamphetamine (methamphetamine,MA) and amphetamine (amphetamine,AMP). Methods C _ (18) column was used with methanol-phosphate buffer as mobile phase, flow rate was 1.0 mL / min, detection wavelength was 215 nm, UV spectra of 190~360nm were collected, and retention time was used as qualitative basis. On the basis of qualitative analysis, a quantitative detection method was established and evaluated. Results the method can be used to separate MA and AMP, correctly and qualitatively. MA was linear in the range of 1.4 渭 g/mL and 270 渭 g/mL, R2X 1, (RSD) and RSD were 2.4, the detection limit was 0.73 渭 g / mL, and the average recovery was 102.5%. The linear range of AMP was 0.9580渭 g/mL, R2O0.99999.The detection limit of RSD was 0.52 渭 g / mL, and the average recovery was 101.7%, both RSD and RSD were 2.3 渭 g 路mL ~ (-1), 0.52 渭 g 路mL ~ (-1) 路min ~ (-1) 路min ~ (-1). Conclusion this method is simple, rapid, accurate and suitable for the detection of MA and AMP.
【作者单位】：四川大学华西基础医学与法医学院四川大学华西基础医学与法医学院法医毒物分析教研室四川大学华西基础医学与法医学院法医毒物分析教研室四川大学华西基础医学与法医学院法医毒物分析教研室四川大学华西基础医学与法医学院法医毒物分析教研室四川大学华西基础医学与法医学院法医毒物分析教研室四川大学华西基础医学与法医学院法医毒物分析教研室
【基金】：国家自然科学基金(批准号30371577)资助
【分类号】：D919

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