Caspase-8在大鼠生前及死后电击伤后多器官的表达

发布时间：2019-01-08 19:24

【摘要】：目的探讨caspase-8蛋白能否用于生前电击与死后电击的鉴别。方法建立大鼠生前与死后电流损伤模型。电击死组于死后即刻取材,生前电击伤组于电击后1h、2h、4h、8h处死;死后电击组大鼠于死后即刻、15min、30min、1h电击大鼠,采用免疫组化法检测大鼠心、肝、脾、肺、肾、骨骼肌、皮肤、脑等脏器caspase-8蛋白的表达。结果生前电击各组大鼠心、肝、肾、脑caspase-8蛋白呈强阳性表达,脾脏与骨骼肌呈阴性表达;而于死后电击各组大鼠中,仅死后即刻电击组大鼠心、肝、肺、肾、骨骼肌、脑caspase-8蛋白呈很微弱的阳性表达,其余各组各脏器均为阴性表达。结论检测各脏器caspase-8蛋白
[Abstract]:Objective to investigate whether caspase-8 protein can be used to differentiate antemortem and postmortem shock. Methods the rat models of antemortem and postmortem current injury were established. In the electrocution group, the samples were taken immediately after death, and those in the antemortem electrocution group were killed at 1 hour, 2 h, 4 h and 8 h, respectively. The rats in the postmortem shock group were exposed to electric shock at 15 min and 30 min for 1 hour. The expression of caspase-8 protein in heart, liver, spleen, lung, kidney, skeletal muscle, skin and brain were detected by immunohistochemical method. Results there was strong positive expression of caspase-8 protein in heart, liver, kidney and brain, but negative expression in spleen and skeletal muscle. However, the expression of caspase-8 protein in the heart, liver, lung, kidney, skeletal muscle and brain of the rats in the shock group only immediately after death showed very weak positive expression, and the other groups showed negative expression in each organ. Conclusion Detection of caspase-8 protein in various organs
【作者单位】：四川大学华西基础医学与法医学院法医病理学教研室四川大学华西基础医学与法医学院法医病理学教研室四川大学华西基础医学与法医学院法医病理学教研室四川大学华西基础医学与法医学院法医病理学教研室四川大学华西基础医学与法医学院法医病理学教研室四川大学华西基础医学与法医学院法医病理学教研室
【分类号】：D919

【相似文献】