法医DNA分析若干问题的研究

发布时间：2019-01-20 08:24

【摘要】：目的对6个短串联重复序列(Short Tandem Repeats,STR)基因座在成都汉族群体中的遗传多态性及其种属特异性进行研究,探讨其在法医学中的应用价值,并为法医DNA分型提供新的候选STR遗传标记。 方法 用Chelex法从110名成都汉族无血缘关系个体的新鲜血以及14种常见动物组织中提取DNA。从GDB数据库中查出6个四核苷酸重复序列D4S2366、D4S2367、D6S474、D6S1281、D2S1396和D20S601,按数据库提供的序列合成引物,经PCR扩增、非变性聚丙烯酰胺凝胶电泳和硝酸银染色技术检测,对110名成都汉族无血缘关系个体的6个STR基因座进行DNA分型,应用POWERSTATS软件对分型结果进行统计分析,获得各基因座基因频率及法医遗传多态性数据。应用所合成的引物对14种常见动物DNA进行PCR扩增和电泳检测,对6个STR基因座的种属特异性进行研究。 结果 D4S2366、D4S2367、D6S474、D6S1281、D2S1396和D20S601基因座在成都汉族群体中等位基因个数分别为7、7、6、7、5和7;观察杂合度分别为0.802、0.708、0.770、0.627、0.542和
[Abstract]:Objective to study the genetic polymorphism and species specificity of six short tandem repeat (Short Tandem Repeats,STR) loci in Chengdu Han population, and to explore their application value in forensic medicine. And to provide a new candidate STR genetic marker for forensic DNA typing. Methods Chelex method was used to extract DNA. from fresh blood and 14 common animal tissues from 110 unrelated individuals of Chengdu Han nationality. Six tetranucleotide repeats D4S2366, D4S2367, D6S474, D6S1281, D2S1396 and D20S601 were identified from GDB database. Primers were synthesized according to the sequences provided by the database, amplified by PCR, detected by non-denatured polyacrylamide gel electrophoresis and silver nitrate staining. Six STR loci of 110 unrelated individuals in Chengdu Han nationality were genotyped by DNA. The results were statistically analyzed by POWERSTATS software, and the genetic polymorphism data of each locus and forensic genetic polymorphism were obtained. The PCR amplification and electrophoretic detection of DNA of 14 common animals were carried out by using the synthesized primers, and the species-specificity of 6 STR loci were studied. Results the number of alleles in D4S2366-D4S2367-D6S474FN D6S1281D2S1396 and D20S601 locus in Chengdu Han population were 70.7080.7270.542 and 0.8020.7080.7080.7270.542, respectively.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：D919

【参考文献】

相关期刊论文 前9条

1 蒋琼橙,伍新尧;人类线粒体DNA异质性及其与法医学的关系[J];中国法医学杂志;2002年01期

2 唐剑频,侯一平,吴谨,李英碧,张健;22号染色体4个STR基因座的遗传多态性及连锁关系[J];中国法医学杂志;2002年04期

3 江斌,郭景元;线粒体DNA测序在法医物证检验中的应用现状及前景[J];法医学杂志;1997年03期

4 周雪平,李英碧,张伟娟,贾政军,侯一平,吴谨;成都汉族D2S2944和D1S2134的遗传多态性和种属特异性研究[J];四川大学学报(医学版);2004年06期

5 侯一平,张思仲;温度调控高效液相色谱探索人类基因组变异的进展[J];中华医学遗传学杂志;2000年03期

6 廖林川,孟海英,侯一平,张思仲,颜有仪,苏智广,李英碧,吴瑾,张霁;用温度调控高效液相色谱探索基因组单核苷酸多态性的方法研究[J];中华医学遗传学杂志;2000年03期

7 张霁,侯一平,吴谨,李英碧,廖林川,王玉芳,魏玉峰;中国人群9号染色体10个STR基因座的遗传多态性及其在法医学中的应用[J];中华医学遗传学杂志;2000年06期

8 梁伟波,吕梅励,贾怡,周斌,刘宽林,陈京辉,廖淼,吴梅筠,张林;中国成都汉族群体线粒体DNA控制区序列多态性研究[J];中华医学遗传学杂志;2004年02期

9 蒋琼橙,伍新尧,区雪玲,孙宏钰;应用DHPLC筛查人类mtDNA高变区I的异质性[J];中山大学学报(医学科学版);2004年01期

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