中国吉林延边地区朝鲜族人群4种STR基因座遗传多态性研究

发布时间：2019-03-14 07:38

【摘要】：【目的】应用PCR扩增、PAGE及银染分型技术对常染色体STR基因座D5S818，D8S1179，D18S51及D21S11的遗传学多态性进行研究，获取其中国吉林延边地区朝鲜族群体遗传学资料，并评估其法医学应用价值。【方法】应用聚合酶链反应(PCR)，聚丙烯酰胺凝胶电泳(PAGE)及银染分型技术，对延边地区109名朝鲜族无关个体进行群体调查，获得四种基因座的群体遗传学资料。【结果】通过群体调查，在D5S818，D8S1179，D18S51及D21S11基因座中分别检出8、8、14及12个等位基因和21、17、42及32种基因型。评估法医学统计结果，杂合度观察值(Ho)分别为0.835、0.780、0.884及0.716，期望值(He)分别为0.798、0.837、0.862及0.767；多态性信息量(PIC)分别为0.768，0.815，，0.848及0.739；个体识别能力(PD)分别为0.916、0.949、0.957及0.910；累积PD为99.9983％，累积匹配概率为1.658×10~(-5)；Hardy-Weinberg平衡检验p值分别为0.421、0.640、0.811及0.837，均符合Hardy-Weinberg平衡。此外将延边地区朝鲜族人群的等位基因分布频率与其他不同种族、民族人群等位基因分布频率之间进行了比较。结果不同种族、民族之间存在着不同程度的差异。最终阐述了建立延边地区朝鲜族人群STR基因座数据库的必要性。【结论】本文所述四个基因座均属于高鉴别力、高杂合度、高信息量的STR基因座，是在法医学个人识别和亲权鉴定中有较高应用价值的遗传标记系统。
[Abstract]:[objective] to study the genetic polymorphisms of autosomal STR loci D5S818, D8S1179, D18S51 and D21S11 by PCR amplification, PAGE and silver staining. The genetic data of Korean population in Yanbian area, Jilin Province, China were obtained, and the value of forensic medicine application was evaluated. [methods] Polymerase chain reaction (PCR),) polyacrylamide gel electrophoresis (PAGE) and silver staining technique were used to investigate the population of 109 unrelated Korean individuals in Yanbian area. Population genetic data of four loci were obtained. [results] there were 8, 8, 14 and 12 alleles and 21, 17, 42 and 32 genotypes in D5S818, D8S1179, D18S51 and D21S11 loci, respectively. The observed values of heterozygosity (Ho) were 0.835,0.780,0.884 and 0.716, and the expected values of (He) were 0.798,0.837, 0.862 and 0.767, respectively. The polymorphic information content (PIC) was 0.768,0.815,0.848 and 0.739 respectively, the individual identification ability (PD) was 0.916,0.949, 0.957 and 0.910, the cumulative PD was 99.9983%, and the cumulative matching probability was 1.658 脳 10 ~ (- 5). The p values of Hardy-Weinberg equilibrium test were 0.421, 0.640, 0.811 and 0.837, respectively, which were in agreement with the Hardy-Weinberg equilibrium. In addition, the allelic frequency of Korean population in Yanbian area was compared with that of other ethnic groups and ethnic groups. Results there are different degrees of differences among different races and nationalities. Finally, the necessity of establishing the database of STR loci in Korean population in Yanbian area was expounded. [conclusion] the four loci described in this paper all belong to STR loci with high discriminative ability, high heterozygosity and high information content. It is a genetic marker system with high application value in forensic personal identification and paternity identification.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919.1

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