过敏性休克死亡豚鼠脏器中IgE、IL-4的表达及其法医学意义

发布时间：2019-03-14 08:50

【摘要】：【目的】寻找法医学鉴定过敏性休克死亡的病理形态学诊断指标。【方法】利用组织芯片技术采用免疫组化方法(SP法)检测35只过敏性休克豚鼠(实验组28只,对照组7只)死后0、6、12、24h4个时间点的心、肝、肺、肾、脾、胃、肠、气管及扁桃体组织中的IgE及IL-4的表达情况。【结果】IgE的表达:实验组肺及气管组织呈阳性表达,并以0、6h表达最强,各时间点的表达强度有统计学意义,P0.01;脾组织呈弱阳性表达,各时间点的表达强度无统计学意义,P0.05;实验组其它脏器及对照组所有脏器均无表达。IL-4的表达:实验组肺、气管及胃组织呈阳性表达,并以0、6h表达最强,各时间点的表达强度有统计学意义,P
[Abstract]:[objective] to search for the pathomorphological diagnostic index of anaphylactic shock death in forensic medicine. [methods] 35 guinea pigs with anaphylactic shock were examined by immunohistochemical method (SP method) with tissue microarray technique (experiment group, 28 guinea pigs, experimental group, 28 guinea pigs). The expression of IgE and IL-4 in the tissues of heart, liver, lung, kidney, spleen, stomach, intestine, trachea and tonsil were detected at 0, 6, 12, 24 hours after death in the control group. [results] the expression of IgE was positive in the lung and trachea of the experimental group. The expression intensity was the strongest at 0 and 6 h, and the expression intensity was statistically significant at each time point (P0.01). There was weak positive expression in spleen tissue, and there was no significant difference in expression intensity at each time point (P0.05). The expression of IL-4 was positive in the lung, trachea and stomach of the experimental group, and the strongest expression was found at 0 and 6 h, the expression intensity of IL-4 was statistically significant at each time point, and the expression of IL-4 was positive in the lung, trachea and stomach of the experimental group.
【作者单位】：井冈山学院医学院井冈山学院医学院井冈山学院医学院井冈山学院医学院井冈山学院医学院井冈山学院医学院井冈山学院医学院
【基金】：江西省吉安市科技局科技计划基金资助项目(200409)
【分类号】：D919

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