4个Y-STR基因座银染复合扩增
发布时间：2019-04-02 01:47
【摘要】：建立一个复合扩增体系,同时扩增DYS391、GATA-A4、GATA-A10和GATA-H44个Y染色体特异性的STR基因座,并用聚丙烯酰胺凝胶电泳和银染显色技术进行基因分型。采用该复合扩增系统调查广东汉族311名无关男性个体的单倍型频率,这4个基因座分别检出5、7、6和5个等位基因,其基因多样性分别为0.4623、0.6972、0.7173、0.6015。共检出98种单倍型,单倍型基因多样性为0.9755。该复合扩增体系在建立Y染色体STR数据库,研究群体遗传和进行法医学鉴定有重要意义。
[Abstract]:A complex amplification system was established to amplify DYS391,GATA-A4,GATA-A10 and GATA-H44 Y chromosome specific STR loci at the same time. Polyacrylamide gel electrophoresis (page) and silver staining were used for genotyping. The haplotype frequencies of 311 unrelated male individuals in Guangdong Han population were investigated by the multiplex amplification system. The four loci were 5, 7, 6 and 5 alleles, respectively. The genetic diversity was 0.4623,0.6972, 0.7173 and 0.6015, respectively, and the genetic diversity was 0.4623,0.6972, 0.7173 and 0.6015, respectively. A total of 98 haplotypes were detected, and the gene diversity of haplotypes was 0.9755. The complex amplification system is of great significance in establishing Y chromosome STR database, studying population heredity and forensic identification.
【作者单位】： 中山大学中山医学院法医物证教研室 中山大学中山医学院法医物证教研室
【分类号】：D919;Q987
[Abstract]:A complex amplification system was established to amplify DYS391,GATA-A4,GATA-A10 and GATA-H44 Y chromosome specific STR loci at the same time. Polyacrylamide gel electrophoresis (page) and silver staining were used for genotyping. The haplotype frequencies of 311 unrelated male individuals in Guangdong Han population were investigated by the multiplex amplification system. The four loci were 5, 7, 6 and 5 alleles, respectively. The genetic diversity was 0.4623,0.6972, 0.7173 and 0.6015, respectively, and the genetic diversity was 0.4623,0.6972, 0.7173 and 0.6015, respectively. A total of 98 haplotypes were detected, and the gene diversity of haplotypes was 0.9755. The complex amplification system is of great significance in establishing Y chromosome STR database, studying population heredity and forensic identification.
【作者单位】： 中山大学中山医学院法医物证教研室 中山大学中山医学院法医物证教研室
【分类号】：D919;Q987
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【共引文献】
相关期刊论文 前10条
1 王永在,张更谦,王艳,张艳霞,，
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