西安枣园西汉早期墓葬出土古酒中菌害分析

发布时间：2018-05-24 03:07

  本文选题：西汉古酒 + 菌害分析　； 参考：《西北大学学报(自然科学版)》2017年06期

【摘要】：针对2003年西安市文物保护考古所发现的西安枣园西汉早期墓葬出土古酒的菌害,分析微生物群落,并对照其成份判断菌种来源,为制定稳妥的古酒灭菌方案提供科学依据。研究使用分子生物学方法,通过培养分离微生物群落,16S r DNA/18S r DNA序列分析,根据同源性比对及系统发育树分析,鉴定出4种优势菌种,其中真菌为淡紫拟青霉菌,3种细菌分别为微球菌、葡萄球菌和考克氏菌属细菌。鉴定出的4种优势菌种与空气微生物中的常见菌种一致;判定古酒菌害原因主要来源于外界空气的交流;提出通过菌落滤除,隔绝空气,以有效抑制古酒中微生物滋生的防治对策。该研究也是16S r DNA/18S r DNA序列分析首次成功在酒类液体文物菌害成份鉴定中应用。
[Abstract]:In view of the bacteria damage of ancient wine unearthed from the early Western Han Dynasty tomb in Xi'an Zaoyuan in 2003, the microbial community was analyzed, and the source of bacteria was judged in comparison with its composition, which provided a scientific basis for the formulation of a sound sterilization plan for ancient wine. By using molecular biology method, four dominant strains were identified by sequence analysis of 16s r DNA/18S r DNA in culture and isolation microbial community, according to homology comparison and phylogenetic tree analysis. Among them, the fungus is Penicillium lilacinus and three kinds of bacteria are micrococcus, Staphylococcus and Corkella respectively. The four dominant strains identified are consistent with the common strains of airborne microbes; the main reasons for the bacteria damage in ancient wine are derived from the exchange of external air; and the idea of isolating the air through colony filtration is put forward. In order to effectively inhibit the microbial breeding in ancient wine control measures. This study is also the first successful application of 16s r DNA/18S r DNA sequence analysis in the identification of the harmful components of alcoholic liquid cultural relics.
【作者单位】： 西安市文物保护考古研究院;西北大学生命科学学院;
【基金】：陕西省生物技术重点实验室基金资助项目(J16S108)
【分类号】：K875.2;TS261.7
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本文编号：1927445