荧光定量PCR解析酸性条件下丙酸氧化菌的演替

发布时间：2017-12-26 16:23

本文关键词：荧光定量PCR解析酸性条件下丙酸氧化菌的演替　出处：《中国环境科学》2017年10期 　论文类型：期刊论文

【摘要】：采用实时荧光定量PCR技术考察了UASB酸性条件下丙酸氧化菌的演替规律.结果表明,至少有3种已鉴定的丙酸氧化菌(Pelotomaculum schinkii,P.propionicum和Smithella propionica)存在于UASB反应器中.在p H值为7.5~7.1时,P.schinkii是主要的丙酸氧化菌群,其数量为(5.0~5.8)×10~3 16SrRNA基因拷贝数/ngDNA,约占检测到丙酸氧化菌总数的90.6%以上.pH从7.1降到6.8导致S.propionica数量显著增加,P.schinkii和S.propionica成为优势菌群,其数量占丙酸氧化菌总数的88.9%.在pH6.5条件下,S.propionica(74.8%)成为优势丙酸氧化菌群.当p H￡6.0时,S.propionica和P.propionicum演替为优势丙酸氧化菌,表明这两种丙酸氧化菌具有较强的耐酸性.当p H￡6.0时,丙酸氧化菌总数随pH降低而明显减少,这可能是厌氧反应器在酸性条件下发生丙酸积累的根本原因之一.
[Abstract]:On the succession of propionate oxidizing bacteria under acidic conditions UASB by real-time fluorescence quantitative PCR. The results show that there are at least 3 kinds of propionate oxidizing bacteria have been identified (Pelotomaculum schinkii, P.propionicum Smithella and propionica) in UASB reactor. The P H value is 7.5 ~7.1, P.schinkii is the main propionate oxidizing bacteria the number of groups, (5.0~5.8) /ngDNA * 10~3 16SrRNA gene copy number, accounting for about more than the total number of propionate oxidizing bacteria detection 90.6%.PH reduced from 7.1 to 6.8 resulted in a significant increase in the number of S.propionica, P.schinkii and S.propionica become the dominant bacteria, the total number of propionate oxidizing bacteria 88.9%. under the condition of pH6.5, S.propionica (74.8%) became the dominant propionate oxidizing bacteria community. When the P H below 6, S.propionica and P.propionicum were the dominant succession of propionate oxidizing bacteria, showed that the two kinds of propionate oxidizing bacteria with strong acid resistance when p H. Below 6, the total number of propionate oxidizing bacteria and decrease with the decrease of pH, which may be one of the root causes of occurrence of anaerobic reactor of propionic acid accumulation in acidic conditions.
【作者单位】：山西大学环境与资源学院;哈尔滨工业大学市政与环境工程学院;
【基金】：国家自然科学基金资助项目(51708341) 天津市水质科学与技术重点实验室开放研究基金资助项目(TJKLAST-ZD-2016-05) 山西省基础研究项目(2015021136,2015021134)
【分类号】：X172
【正文快照】： 厌氧生物处理技术广泛用于处理各种有机废弃物,同时回收甲烷作为清洁能源[1-3].有机物厌氧发酵可产生大量的中间代谢产物,包括丙酸[4].从热力学的角度看,丙酸降解是一个高度吸能的过程,很难自发进行.所以,当系统遭受温度、有机负荷率、毒性物质等冲击时,丙酸降解受到抑制,并在

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