焦化废水活性污泥PAH双加氧酶基因多样性分析

发布时间：2017-12-27 21:09

本文关键词：焦化废水活性污泥PAH双加氧酶基因多样性分析　出处：《中国环境科学》2017年01期 　论文类型：期刊论文

【摘要】：为分析焦化废水活性污泥中降解PAH双加氧酶的多样性,利用16Sr DNA-PCR-DGGE方法,以实际焦化废水好氧单元活性污泥总DNA为模板,通过引物对污泥中的双加氧酶基因进行了克隆表达和多样性分析.结果表明,以活性污泥总DNA为模板,利用引物RHD-GN-610F和RHD-GN-916R扩增后有明显的产物,产物大小约为300bp;DGGE指纹图谱显示PCR产物有8条分离条带,丰度分别为2.99%、8.16%、20.75%、28.50%、8.62%、7.26%、10.62%和13.10%;条带经切胶回收PCR扩增后出现明显的扩增产物,TA克隆后成功测试出4条序列,长度分别为305bp,298bp,334bp和294bp,表明焦化废水活性污泥中存在不同降解PAH的RHD酶.这些结果为焦化废水中PAHs的风险评估及其o[在生物降解提供理论基础.
[Abstract]:For the analysis of diversity of PAH degradation of activated sludge in the coking wastewater dioxygenase, using the 16Sr DNA-PCR-DGGE method in coking wastewater activated sludge aerobic unit total DNA as template, primers were cloned by the dioxygenase gene expression analysis in sludge and diversity. The results show that in activated sludge using DNA as template, primers RHD-GN-610F and RHD-GN-916R were amplified product, the product size is about 300bp; DGGE fingerprint showed that PCR products have 8 separate bands, abundance were 2.99%, 20.75%, 8.16%, 8.62%, 7.26%, 10.62%, 28.50% and 13.10%; strip by gel extraction after PCR amplification the amplification products obviously, after TA cloning successfully tested 4 sequences, the length were 305bp, 298bp, 334bp and 294bp, showed that the RHD enzyme has different PAH degradation of activated sludge in the coking wastewater. The results of PAHs in Coking Wastewater The risk assessment and its o[provide a theoretical basis for biodegradation.
【作者单位】：安康学院旅游与资源环境学院;中国科学院过程工程研究所绿色过程与工程重点实验室;
【基金】：安康学院高层次人才科研专项经费(2016AYQDZR09) 国家自然科学基金项目(31370281) 化学工业废水处理污泥污染特征与污染风险控制研究(201509053)
【分类号】：X784
【正文快照】： 程工程研究所绿色过程与工程重点实验室,北京100190)PAHs是一类由稠合芳环,不含取代基或杂原子组成的有毒有害化合物,这类物质常以混合的形式存在,广泛分布于不同性质的水环境介质中,如生活废水和工业废水中.在废水的处理过程中PAHs会大量积存于初沉池污泥、活性污泥、消化污

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