锰胁迫对苍耳种子萌发及幼苗生理生化特性的影响

发布时间：2018-01-10 14:30

本文关键词：锰胁迫对苍耳种子萌发及幼苗生理生化特性的影响　出处：《草业学报》2017年11期 　论文类型：期刊论文

【摘要】：为探明苍耳对锰胁迫适应的生理生化机制,以苍耳种子为试验材料,采用培养皿滤纸法,研究了不同浓度锰(0,1000,5000,10000,15000和20000μmol/L)胁迫对苍耳种子萌发、幼苗生长及生理生化特性的影响。结果表明:1)1000~5000μmol/L锰胁迫显著提高了苍耳种子的萌发率,增加了幼苗的芽长和根系活力;当浓度5000μmol/L时,发芽势、发芽指数、活力指数以及根长、鲜重、干重、根冠比均显著降低;2)随着锰浓度的升高,苍耳幼苗叶片中可溶性糖、可溶性蛋白和脯氨酸含量以及超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、愈创木酚过氧化物酶(GPX)、过氧化氢酶(CAT)活性均呈现先增加后下降的变化趋势;3)苍耳幼苗体内活性氧和膜脂过氧化物含量随着锰浓度的升高显著增加,超氧阴离子自由基(O2·-)产生速率、羟自由基(OH·)浓度、H2O2和MDA含量分别增加了154.69%、47.29%、100.09%和200.96%。研究结果显示,苍耳对锰胁迫具有较强的耐性,可作为锰矿废弃地修复的备选植物。
[Abstract]:For the physiological and biochemical mechanism of proven Xanthium to manganese stress adaptation, with cocklebur seeds as experimental materials, using Petri dish filter paper method, the effects of different concentrations of manganese (010005000100001500 and 20000 mol/L) stress on Germination of Xanthium seeds, affect the growth and physiological and biochemical characteristics of the seedlings. The results showed that: 1) 1000~5000 mol/L manganese stress significantly increased the germination rate of seeds of Xanthium, increased seedling shoot length and root vigor; when the concentration is 5000 mol/L, the germination potential, germination index, vigor index, root length, fresh weight, dry weight, root shoot decreased; 2) with increasing manganese concentration, soluble sugar in seedling leaves of Xanthium sibiricum, soluble protein and proline content and superoxide dismutase (SOD), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), catalase (CAT) activity showed a trend of first increasing and then decreasing; 3) of Xanthium sibiricum Seedlings of active oxygen and lipid peroxide content with increasing manganese concentration significantly increased, superoxide anion radical (O2 -) production rate, hydroxyl radical (OH) concentration, H2O2 and MDA content increased by 154.69%, 47.29%, 100.09% and the 200.96%. study showed that tPA had a strong tolerance to manganese stress that can be used as alternative plant manganese mine wasteland restoration.

【作者单位】：中南林业科技大学生命科学与技术学院;南方林业生态应用技术国家工程实验室;
【基金】：湖南省教育厅重点项目(14A156) 湖南省研究生科研创新项目(CX2017B400) 中南林业科技大学研究生科技创新基金项目(CX2017A06)资助
【分类号】：Q945.78;X503.233
【正文快照】： 锰(Mn)是维持植物正常生命活动所必需的微量营养元素,适量的锰能活化植物体内的酶系统,促进植物的生长;而过量的锰则会诱导活性氧合成积聚、破坏细胞结构,进而对植物产生毒性效应,甚至通过食物链危害人体健康[1-2]。当前,由于锰矿开采、冶炼等工业活动的增加,锰污染程度加深、

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