安徽某铁矿排土场废矿石中产酸微生物群落

发布时间：2018-01-11 13:38

本文关键词：安徽某铁矿排土场废矿石中产酸微生物群落　出处：《环境科学》2017年11期 　论文类型：期刊论文

【摘要】：安徽某铁矿排土场是该铁矿主要的废矿石堆放地.这些露天堆放的废矿石经过长期的氧化,于20世纪70年代在排土场中央形成了一个大型酸水库.此外,在该酸水库周边还存在一些近年形成的小范围酸性矿山废水(acid mine drainage,AMD).为研究该区域废矿石的产酸潜力及产酸微生物群落,采集了酸水库周边长期堆放的废矿石以及两处新形成AMD周边的废矿石样品共6个,分别为酸水库边1号位裸露1LL,新形成的AMD 5号位旁5J、5Y,6号位旁6-1、6-2和6-3.物化分析表明6个样品酸性均很强,样品1LL的pH最高,也仅为2.77,而其他5个样品的pH均在2.60以下.1LL的电导率为0.32m S·cm~(-1),5号和6号位样品的电导率则高达2.25~7.08 m S·cm~(-1),证明其中含有更高浓度的阴阳离子.1LL中Fe~(2+)的含量为0.80 mg·kg-1,而其它几个样品的含量则为2.91~33.40 mg·kg~(-1),证明1号的样品经过长期的风化,Fe~(2+)基本上已被氧化成Fe~(3+).16S rRNA基因高通量测序表明,5号位和6号位样品中的嗜酸菌主要属于γ-Proteobacteria、Firmicutes、Nitrospira.1号位样品中的嗜酸菌主要属于Actinobacteria、Acidobacteria、Chloroflexi.5号位和6号位样品含有大量的铁硫氧化菌,如Sulfobacillus、Leptospirillum、Acidithiobacillus,证明其中发生着旺盛的产酸过程;而1号位样品1LL微生物群落相对复杂,但基本不存在铁硫氧化菌,原因可能是酸水库周边长期堆放样品中的铁和硫都已经氧化殆尽,基本上不再产酸.统计学分析结果表明,1号位样品1LL与另外5个样品微生物组成存在明显差异,说明不同物化条件对微生物群落结构有决定性影响.
[Abstract]:An iron ore dump in Anhui is the main waste ore deposit of the iron ore . The waste ores stacked in the open pit form a large acid reservoir in the center of the dump in the 1970s . In addition , there are some small - scale acid mine drainage ( AMD ) formed in recent years at the periphery of the acid reservoir . In order to study the acid - producing potential and acid - producing microbial communities of the waste ores in the region , 6 samples were collected for the long - term stacking of waste ores at the periphery of the acid reservoir , and 6 - 1 , 6 - 2 and 6 - 3 respectively . The results showed that the acidophilic bacteria in the samples of No . 5 and No . 6 had been oxidized to Fe ~ ( 3 + ) . The results showed that the samples of No . 5 and 6 were mainly oxidized to Fe ~ ( 3 + ) . The results showed that the samples of No . 1 and No . 6 samples had been oxidized to Fe ~ ( 3 + ) . The results showed that there was a significant difference between the samples 1LL and the other five samples . The results showed that the different physicochemical conditions had a decisive influence on the structure of microbial communities .

【作者单位】：中国地质大学(北京)水资源与环境学院;北京市环境保护监测中心;
【基金】：中央高校基本科研业务费项目(2652015114)
【分类号】：X172;X753
【正文快照】： 酸性矿山废水(acid mine drainage,AMD)是硫化矿物暴露于地表,与水、大气、微生物相互作用,发生氧化性溶解形成的,p H通常小于3,且含有高浓度重金属如Fe、Al、Cu等和SO2-4,危害十分严重[1].在自然界,酸性矿山废水主要由黄铁矿(Fe S2)氧化形成[2].黄铁矿的氧化过程为:2Fe S2+7O

【相似文献】