细菌对城市污水中小球藻生长和油脂积累的影响

发布时间：2018-01-11 14:32

本文关键词：细菌对城市污水中小球藻生长和油脂积累的影响　出处：《环境科学》2017年10期 　论文类型：期刊论文

【摘要】：利用城市污水培养微藻,可在实现污水无害化处理的同时,培养微藻回收生物质能源.污水为微藻的培养提供氮、磷等营养组分和所需水源,同时污水中的细菌可分解污水中的有机物产生CO_2,为微藻提供生长所需碳源.菌藻混合培养既可以收获藻类,又可以净化污水,由于城市污水含有大量的原生菌类,且微藻与细菌之间存在着互生、拮抗等复杂的相互关系,因此,需要筛选出既能够适应于城市污水又能促进微藻生长和油脂积累的优势菌种.本文从不同来源的13种细菌中筛选出2种能够显著促进蛋白核小球藻(Chlorella pyrenoidosa)生长和油脂积累细菌,并分析了微藻培养结束后城市污水的菌群结构.结果表明:污水中光合细菌初始吸光度D600为0.01,W4菌初始吸光度D_(600)为0.02时,对小球藻的干重和油脂产量促进作用最显著,油脂产量分别可达0.114 g·L~(-1)、0.113 g·L~(-1),油脂产量比空白对照组分别提高了22.58%、21.50%.通过对生成的脂肪酸甲酯进行气相色谱分析,结果显示光合细菌和W4菌的添加并未改变小球藻脂肪酸成分,但提升了单不饱和脂肪酸的含量,有利于提升所得生物柴油的品位.培养结束后污水的菌群结构分析显示投加细菌会降低污水中菌群的丰富度和多样性,初步判断是投加的菌在藻液中能够成为优势菌群,且实验组中丛毛单胞菌属(Comamonas)和假单胞菌属(Pseudomonas)的丰度大于对照组.
[Abstract]:Cultivation of microalgae using city sewage can be achieved at the same time, in the sewage harmless treatment, recycling of microalgae biomass energy. Sewage nitrogen for microalgae culture, phosphorus and other nutrient components and required water to produce CO_2 organic matter and bacteria in the sewage can be decomposed in sewage, providing carbon source is required for the growth of microalgae. The mixed culture of bacteria and algae can harvest the algae, and can purify sewage, the city sewage contains a large number of native fungi, and between microalgae and bacteria are alternate, relationship, antagonistic complex therefore needs to be selected not only to be able to adapt to the city sewage and can promote the dominant species of microalgae growth and lipid accumulation in this paper. Different sources of 13 kinds of bacteria screened 2 could significantly promote the Chlorella pyrenoidosa (Chlorella pyrenoidosa) growth and lipid accumulation of bacteria, and analyzed the city sewage after cultivation of microalgae The bacterial community structure. The results showed that the photosynthetic bacteria in wastewater initial absorbance of D600 was 0.01, W4 was the initial absorbance of D_ (600) is 0.02, the dry weight of Chlorella and oil yield promoting effects, oil yield of up to 0.114 G - L~ (-1), 0.113 G - L~ (-1), oil the lipid yield than the blank control group were increased by 22.58%, 21.50%. by fatty acid methyl ester generated by gas chromatography. The results showed that the addition of photosynthetic bacteria and W4 bacteria did not change the fatty acid composition of Chlorella, but to enhance the content of monounsaturated fatty acids, conducive to enhancing the biodiesel culture taste. After the end of the bacterial community structure analysis showed that adding sewage bacteria can reduce the sewage in the richness and diversity of flora, the preliminary judgment is that adding bacteria in algae solution can become the dominant bacteria in the experimental group, and Comamonas (Comamonas) and Pseudomonas (Pseudo The abundance of Monas is greater than that of the control group.

【作者单位】：哈尔滨工业大学深圳研究生院土木与环境工程学院;
【基金】：深圳市科技计划资助项目(JCYJ20150529114024234)
【分类号】：X703.1
【正文快照】： 随着能源危机与环境污染的加剧,微藻生物柴油越来越受到各国研究者的关注[1~3].就整体工艺而言,微藻生物柴油的生产包括藻种选育、微藻培养、微藻收获、油脂提取与转脂.微藻培养是其中一个重要环节,在微藻培养过程中,利用淡水培养微藻需消耗大量淡水资源,有悖于当前淡水资源紧

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