潜流人工湿地基质结构与微生物群落特征的相关性

发布时间：2018-01-11 15:23

本文关键词：潜流人工湿地基质结构与微生物群落特征的相关性　出处：《环境科学》2017年09期 　论文类型：期刊论文

【摘要】：为查明造成不同基质结构人工湿地净化性能差异的微生物因素,利用高通量测序技术,研究水平潜流人工湿地基质结构与微生物群落结构的相关性.结果表明,3个对比人工湿地系统中,从床体表层到底层渗透系数逐渐升高的六层结构人工湿地(CW6)净化性能最高,其出水中COD、TN、NO-3-N及NH_4~+-N平均浓度分别为39、11、0.35及4 mg·L~(-1),其后为三层结构人工湿地(CW3),而单层结构人工湿地(CW1)的净化效果最差,平均出水浓度分别为95、21、0.60及12 mg·L~(-1).高通量测序结果显示,多层结构人工湿地中微生物OTUs略低于单层结构湿地,但其优势菌门变形菌门(Proteobacteria)和其属水平上的硝化与反硝化细菌的相对丰度却显著高于单层湿地.PCA和丰度热图分析结果显示,CW3和CW6床体中变形菌门(Proteobacteria)属水平上的微生物存在显著的空间分布差异,从而有利于污染物的分区降解,而CW1各样点间的微生物群落结构无显著性差异.
[Abstract]:In order to find out the microbiological factors that cause the difference of purification performance of constructed wetland with different substrate structure, the correlation between substrate structure and microbial community structure of horizontal subsurface flow constructed wetland was studied by high-throughput sequencing technique. Among the three comparative constructed wetland systems, the six-layer constructed wetland (CW6), whose permeability coefficient increased from bed surface layer to bottom layer, had the highest purification performance, and its COD _ (+) TN in effluent was the highest. The average concentrations of NO-3-N and NH4 ~ -N were 39 ~ 110.35 and 4 mg 路L ~ (-1), respectively, followed by a three-layer constructed wetland (CW3). The purification efficiency of CW1 was the worst, with the average effluent concentration of 95 ~ 21g 路L ~ (-1) and 12 mg 路L ~ (-1) 路L ~ (-1), respectively. The results of high-throughput sequencing showed that CW1 had the worst purification effect. The microbial OTUs in multi-layer constructed wetland was slightly lower than that in single-layer constructed wetland. But its dominant phylum, Proteobacteria). The relative abundance of nitrification and denitrifying bacteria was significantly higher than that of monolayer wetland. PCA and abundance heat map analysis showed that the relative abundance of nitrifying and denitrifying bacteria was higher than that of monolayer wetland. There were significant spatial distribution differences between Proteobacteria (Proteobacteria) of Proteobacteria in CW3 and CW6 bed, which was beneficial to the degradation of pollutants in different zones. However, there was no significant difference in the structure of microbial community between various points of CW1.
【作者单位】：桂林理工大学广西矿冶与环境科学实验中心;广西环境污染控制理论与技术重点实验室;
【基金】：国家自然科学基金项目(41404116,51638006,51408147) 广西科技三项(桂科合1599005-2-2) 广西高等学校高水平创新团队及卓越学者计划项目
【分类号】：X172;X37
【正文快照】： 桂林541004)水平潜流人工湿地(horizontal subsurface flowconstructed wetlands,HSSFCWs),已被广泛用于工业、农业、生活污水以及城市地表径流的处理中[1~3].前人利用数值模拟方法结合实验研究结果表明,基质结构对人工湿地(constructed wetlands,CWs)水力性能及净化效率影响

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