室内外雾霾暴露人群血清IL-6、IL-10、TNF-α水平的变化

发布时间：2018-01-12 01:31

本文关键词：室内外雾霾暴露人群血清IL-6、IL-10、TNF-α水平的变化　出处：《河北医科大学》2017年硕士论文　论文类型：学位论文

更多相关文章： 雾霾暴露 IL-6 IL-10 TNF-α 危害

【摘要】：目的:测定室内外雾霾暴露人群血清白介素6、白介素10(interleukin,IL-6、IL-10)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)水平,观察雾霾高暴露、低暴露情况下对人体炎症因子的影响,为采取适当防护措施减少细颗粒物(particulate matter with an aerodynamic diameter of 2.5μm or less,PM2.5)对健康危害提供理论依据。方法:选取河北省人民医院2015年10月至2015年12月两批体检人群,分别为干部职员等办公室人员、公交司机,发放调查问卷,了解性别、年龄、吸烟状况、居住环境、室外暴露时间、防护措施采取情况,完成问卷调查后清晨空腹抽取静脉血,酶联免疫吸附法(enzyme-linked immunosorbent assay,Elisa)测定血清IL-6、IL-10、TNF-α水平。结果:1室内组与室外组人群一般情况的比较:室内组共88人,男性69人(78.4%),女性19人(21.6%),年龄分布为46.2±3.31岁,目前吸烟者为48人(54.5%);室外组共90人,男性73人(81.1%),女性17人(18.9%),年龄分布为46.6±3.33岁,目前吸烟者为52人(57.8%)。两组人群的性别构成(χ2=0.201,P=0.654)、年龄(t=㧟0.750,P=0.454)、吸烟率(χ2=0.189,P=0.664)差异无统计学意义。研究人群大部分居住在城区:室内组居住在城区者为81人(92%),远郊3人(3.4%),近郊4人(4.5%);室外组居住在城区者为76人(84.4%),远郊9人(10%),近郊5人(5.6%)。调查使用空气净化器、雾霾期间合理开门窗、佩戴口罩等防护措施的情况室内组40人(45.5%)、室外组32人(35.6%),两组人群的居住环境(χ2=3.204,P=0.192)、采取防护措施率(χ2=1.810,P=0.179)差异无统计学意义;2采集血样1年前石家庄市空气中PM2.5浓度数据以及室内组、室外组暴露时间的对比:2015年石家庄市PM2.5浓度日均水平波动在6~391μg/m3,平均浓度是71μg/m3。同一时期室内组人群每天的室外暴露时间在1~6小时之间,平均暴露时间为2小时,室外组人群每天的室外暴露时间在8~13小时之间,平均暴露时间10小时,室外组明显高于室内组(Z=㧟11.634,P=0.000);3室内组与室外组血清IL-6水平变化:室内组、室外组血清IL-6平均水平分别为3.94±0.48 pg/ml、17.05±2.30 pg/ml,室外组血清IL-6高于室内组(t=㧟52.925,P为0.000);4室内组与室外组血清IL-10水平变化:室内组、室外组血清IL-10平均水平分别为3.27±0.51 pg/ml、22.39±1.62 pg/ml,室外组血清IL-10高于室内组(t=㧟106.904,P为0.000);5室内组与室外组血清TNF-α水平变化:室内组、室外组血清TNF-α平均水平分别为25.24±2.15 pg/ml、106.13±9.27 pg/ml,室外组血清TNF-α高于室内组(t=㧟80.618,P为0.000)。结论:室外组雾霾暴露人群血清IL-6、IL-10、TNF-α高于室内组,PM2.5空气污染增强人体炎性反应,减少室外雾霾暴露可能取得健康获益。
[Abstract]:Objective: to determine serum interleukin 6 (IL 6) and interleukin 10 interleukin 6 (IL 6) in people exposed to haze in and out of the room. The level of tumor necrosis factor- 伪 (TNF- 伪) was measured to observe the effect of high exposure to haze and low exposure to TNF- 伪. To reduce fine particulate matter for appropriate protective measures (. Particulate matter with an aerodynamic diameter of 2.5 渭 m or less. Methods: two groups of physical examination people from October 2015 to December 2015 in Hebei Provincial people's Hospital were selected as cadres and clerks. Bus drivers, the distribution of questionnaires to understand gender, age, smoking status, living environment, outdoor exposure time, protective measures to take the situation, completed the questionnaire after the fasting blood sampling in the morning. Serum IL-6 IL-10 was determined by enzyme-linked immunosorbent assay (Elisa). Results the general situation of the indoor group and the outdoor group were compared. There were 88 people in the indoor group, 69 males 78.4%, and 19 females 21.60.Results: the average level of TNF- 伪 in the indoor group was 78.4% and that in the female group was 21.60.Results there were 88 cases in the indoor group. The age distribution was 46.2 卤3.31 years old. There were 90 persons in the outdoor group, 73 in males and 18.9 in females. The age distribution was 46.6 卤3.33 years old. The sex composition of the two groups of smokers was 57.8% (蠂 2 0.201P 0.654). The smoking rate and smoking rate were 0.750 and 0.454 respectively (蠂 2 + 0.189). There was no significant difference (P < 0. 664). Most of the people in the study lived in urban areas: 81 in the indoor group and 3 in the outer suburbs. 4 in the suburbs; In the outdoor group, 76 people lived in the urban area, 9 in the outer suburbs and 5 in the suburbs. The air purifier was used in the investigation, and the doors and windows were opened reasonably during the haze period. There were 40 persons in indoor group and 35. 6 in outdoor group. The living environment of both groups was 35.6% (蠂 2 3. 20 4 P < 0. 192). There was no significant difference in the rate of protective measures (蠂 ~ 2 ~ (1.810) P ~ (0.179)). 2Blood samples were collected one year ago in Shijiazhuang City air PM2.5 concentration data and indoor group. Comparison of exposure time in outdoor group: in 2015, the daily average level of PM2.5 concentration in Shijiazhuang city fluctuated from 6 ~ 391 渭 g / m ~ 3 to 6 渭 g / m ~ 3. The average concentration was 71 渭 g / m ~ (3). In the same period, the outdoor exposure time of the indoor group was between 1 and 6 hours, and the average exposure time was 2 hours. The outdoor exposure time of outdoor group was between 813 hours and the average exposure time was 10 hours. The outdoor group was significantly higher than the indoor group. 11.634 Pu 0.000; 3 the changes of serum IL-6 level in indoor group and outdoor group: the average level of serum IL-6 in indoor group and outdoor group was 3.94 卤0.48 pg/ml. The serum IL-6 in outdoor group was higher than that in indoor group (17.05 卤2.30 PG / ml). 52.925 (P = 0.000); 4 the changes of serum IL-10 level in indoor group and outdoor group: the average level of serum IL-10 in indoor group and outdoor group was 3.27 卤0.51 pg/ml. 22.39 卤1.62 PG / ml, serum IL-10 in outdoor group was higher than that in indoor group. 106.904 (P = 0.000); 5 changes of serum TNF- 伪 level in indoor group and outdoor group: the average level of TNF- 伪 in indoor group and outdoor group was 25.24 卤2.15 pg/ml. 106.13 卤9.27 PG / ml, serum TNF- 伪 in outdoor group was higher than that in indoor group. Conclusion: the level of serum IL-6 and IL-10 TNF- 伪 in outdoor haze exposed population is higher than that in indoor group. Reducing outdoor haze exposure may benefit health.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R122

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