针铁矿对模拟废水厌氧产甲烷过程的作用研究

发布时间：2018-01-23 03:38

本文关键词： 厌氧消化针铁矿水力负荷甲烷微生物群落　出处：《合肥工业大学》2017年硕士论文　论文类型：学位论文

【摘要】：厌氧产甲烷是有机废弃物资源化的有效途径之一,已经被广泛应用于处理工业废水、城市生活垃圾、农业废弃物、禽畜粪便等。但目前存在产甲烷效率低、转化速率较慢等缺点。本研究尝试利用自然界中广泛存在的针铁矿为添加剂强化废水产甲烷过程。以乙酸钠和葡萄糖和为碳源模拟有机废水,研究了针铁矿对有机废水厌氧产甲烷的作用,考察了不同水力负荷条件下对连续流厌氧产甲烷反应器消化体系甲烷产量及产甲烷速率的作用,并进一步分析了微生物群落演变过程,得到的主要结论如下:1)在序批式反应器中添加针铁矿或Fe~(3+)/Fe~(2+)能够促进模拟废水的产甲烷过程,而且针铁矿的强化作用更高。针铁矿在系统中的变化不显著,而添加Fe~(3+)/Fe~(2+)的系统中形成了蓝铁矿(FePO_4)或菱铁矿(FeCO_3)。2)在连续流反应器中,当水力负荷为0.91-1.80 g/L/d(葡萄糖)时,针铁矿的促进效果最为显著。当OLR为1.80 g/L/d时,系统产甲烷量以及产甲烷效率最高,添加针铁矿反应器和对照组反应器产甲烷量分别为247和209 mL/L/d。3)微生物群落分析结果显示,针铁矿促进了反应体系中Clostridium(梭菌属)和Methanothrix(产甲烷丝状菌属)的生长,对有机物的产酸发酵以及产甲烷过程具有促进作用。4)不同水力负荷条件下Methanothrix均为产甲烷古菌的优势菌属。而细菌群落分布随水力负荷变化波动较大,多数细菌为产酸发酵型菌。针铁矿反应器细菌的多样性指数较高,稳定的群落结构可能有利于甲烷的生成。
[Abstract]:Anaerobic methanogenesis is one of the effective ways to recycle organic wastes, which has been widely used in the treatment of industrial wastewater, municipal solid waste, agricultural waste, livestock manure, etc. But at present, methane production efficiency is low. This study attempted to use goethite as additive to enhance the process of waste aquatic methane. Sodium acetate and glucose were used as carbon source to simulate organic wastewater. The effect of goethite on anaerobic methanogenesis of organic wastewater was studied. The effects of different hydraulic loads on methane production and methanogenic rate in digested system of continuous flow anaerobic methanogenic reactor were investigated. The evolution process of microbial community was further analyzed. The main conclusions are as follows: (1) addition of goethite or Fe~(3 / Fean2 in sequencing batch reactor can promote the methanogenic process of simulated wastewater. Moreover, goethite has a higher strengthening effect. The change of goethite in the system is not obvious. However, in the system with the addition of Fe~(3 / Feo ~ (2), the bluestone / FePO4) or siderite / FeCO3 / 2) are formed in the continuous flow reactor. When the hydraulic load is 0.91-1.80 g / L / d (glucose), goethite is the most effective, and the OLR is 1.80 g / L / d. The results of microbial community analysis showed that the methanogenic capacity and methanogenic efficiency of the system were the highest, and the methanogenic capacity of the adding goethite reactor and the control reactor were 247 and 209 mL / L / 3, respectively. Goethite promoted the growth of Clostridium (Clostridium) and Methanothrix (methanothrix) in the reaction system. It can promote acidogenic fermentation and methanogenic process of organic compounds. 4). Under different hydraulic loading conditions, Methanothrix was the dominant genus of methanogenic palaeomycetes, and the distribution of bacteria community fluctuated greatly with hydraulic load. Most of the bacteria were acidogenic fermentative bacteria, and the diversity index of bacteria in goethite reactor was higher, and stable community structure might be beneficial to methane production.
【学位授予单位】：合肥工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TQ221.11;X703

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