五种豆科药用植物种子超低温保存技术研究

发布时间：2018-02-08 08:03

本文关键词： 超低温冷冻豆科药用植物玻璃化含水量　出处：《生物资源》2017年01期 　论文类型：期刊论文

【摘要】：以豆科药用植物降香檀、决明、含羞草、灰毛豆和猪屎豆的成熟种子为材料,探讨含水量对其发芽率的影响,以及超低温冷冻方式对种子超低温保存的影响。结果表明,降香檀、决明、猪屎豆和灰毛豆种子发芽率均随含水量的下降而从80%左右降至20%以下,而含羞草种子含水量低于10%时,其发芽率仍在75%以上。经超低温冷冻后,五种豆科药用植物种子发芽率较对照组均有显著差异;适宜的含水量下,种子经过超低温冷冻后其发芽率与对照组差异不显著,甚至高于对照组。三种冷冻方法中,玻璃化冷冻法更适合降香檀种子的超低温保存,缓慢冷冻法更适合猪屎豆种子的超低温保存,快速冷冻法适合于决明种子、灰毛豆种子和含羞草种子的超低温保存。由此可知,液氮超低温冷冻法保存降香檀等五种豆科药用植物种子是可行的。
[Abstract]:The effects of water content on germination rate and the effect of cryopreservation on the seed germination rate were studied by using mature seeds of the medicinal plants of Leguminosae, Cassia, Mimosa, Soybean and Crotalaria. The results showed that: 1. The germination rate of seeds decreased from 80% to 20% with the decrease of water content, while the germination rate of Mimosa seeds was still above 75% when the water content of the seeds was lower than 10. After cryopreservation, the germination rate of seeds decreased from 80% to 20%. The germination rate of seeds of five legume medicinal plants was significantly different from that of the control group, and the germination rate of the seeds after cryopreservation was not significantly different from that of the control group under the suitable water content, even higher than that of the control group. The vitrification method is more suitable for the ultra-low temperature preservation of the seeds, the slow freezing method is more suitable for the cryopreservation of Crotalaria davidii seeds, and the rapid freezing method is suitable for the ultra-low temperature preservation of the seeds of Cassia sinensis, Soybean and Mimosa. It is feasible to preserve the seeds of five legume medicinal plants by cryopreservation with liquid nitrogen.
【作者单位】：中国医学科学院药用植物研究所海南分所;
【基金】：中央高校基本科研业务费专项(协和青年基金)(3332016071) 海南省自然科学基金(20153147) 公益性行业(农业)科研专项(201303117)
【分类号】：S567

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